Insights Into the Molecular Genetics of Hexose Transporter Gene Regulation in Saccharomyces Cerevisiae PDF Download
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Author: Kevin L. Dietzel
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Book Description
Author: Kevin L. Dietzel
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Book Description
Author: Xiexiong Deng
Publisher:
ISBN: 9780438023802
Category : Electronic dissertations
Languages : en
Pages : 151
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Book Description
Author: William Jeremy Blake
Publisher:
ISBN:
Category :
Languages : en
Pages : 244
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Book Description
Abstract: Dissecting the complex circuitry of the cell is a principal challenge in the post-genomic era. Advances in the fields of molecular biology and bioinformatics have made this problem more amenable to an engineering approach, where components are characterized and assembled to elicit predefined behaviors. In this work we implement an approach which combines computational and experimental techniques to develop artificial gene networks that are used to explore the molecular and biochemical interactions which coordinate cell function. We present the design, construction, and analysis of robust genetic switches in the single-celled eukaryote Saccharomyces cerevisiae , including a bistable switch analogous to an electrical toggle switch. The S. cerevisiae genetic toggle switch allows reversible control of target gene expression in response to an externally applied chemical signal, with up to a 100-fold difference between stable ON and OFF target gene expression states. This functional bistable switch has potential biotechnological and biomedical applications. In addition, we present an experimental and theoretical investigation of the origins and consequences of stochasticity in gene expression in eukaryootc cells. By utilizing well-characterized artificial gene networks, we modulate the expression of a single gene in S. cerevisiae and combine our results with in silico stochastic models of specific eukaryotic transcription mechanisms. A key result of our combined approach addresses the sensitivity of noise in eukaryotic gene expression to the level of transcription and proposes a possible role for the transcription apparatus assembly in modulating such noise. Further, we demonstrate a plausible mechanism for heritable changes in target gene expression whereby increased levels of noise in the expression of a regulatory protein causes a population of isogenic cells to exhibit bistable expression states. Together, our findings address the origins, propagation, and consequences of noise in the expression of eukaryotic genes, and provide insight into the biological design principles involved in noise modulation.
Author: Justin Michael Keener
Publisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 72
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Book Description
Author: Friedrich K. Zimmermann
Publisher: CRC Press
ISBN: 9781566764667
Category : Technology & Engineering
Languages : en
Pages : 590
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Book Description
Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.
Author: Helmut König
Publisher: Springer
ISBN: 3319600214
Category : Technology & Engineering
Languages : en
Pages : 711
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Book Description
The second edition of the book begins with the description of the diversity of wine-related microorganisms, followed by an outline of their primary and energy metabolism. Subsequently, important aspects of the secondary metabolism are dealt with, since these activities have an impact on wine quality and off-flavour formation. Then chapters about stimulating and inhibitory growth factors follow. This knowledge is helpful for the growth management of different microbial species. The next chapters focus on the application of the consolidated findings of molecular biology and regulation the functioning of regulatory cellular networks, leading to a better understanding of the phenotypic behaviour of the microbes in general and especially of the starter cultures as well as of stimulatory and inhibitory cell-cell interactions during wine making. In the last part of the book, a compilation of modern methods complete the understanding of microbial processes during the conversion of must to wine.This broad range of topics about the biology of the microbes involved in the vinification process could be provided in one book only because of the input of many experts from different wine-growing countries.
Author: Jure Piškur
Publisher: Springer
ISBN: 3642550134
Category : Science
Languages : en
Pages : 328
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Book Description
Yeast is one of the most studied laboratory organisms and represents one of the most central models to understand how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A central biochemical activity in the yeast cell is the metabolism of carbon compounds, providing energy for the whole cell, and precursors for any of the final fermentation products. A complex set of genes and regulatory pathways controls the metabolism of carbon compounds, from nutrient sensing, signal transduction, transcription regulation and post-transcriptional events. Recent advances in comparative genomics and development of post-genomic tools have provided further insights into the network of genes and enzymes, and molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products. This book provides a dozen of most comprehensive reviews on the recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology relevant topics.
Author: Christine Guthrie
Publisher:
ISBN: 9780123106704
Category : Molecular biology
Languages : en
Pages : 933
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Book Description
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein
Author: Stefan Hohmann
Publisher: Springer Science & Business Media
ISBN: 3540456112
Category : Science
Languages : en
Pages : 398
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Book Description
Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.
Author: Ayman Amer Eissa
Publisher: BoD – Books on Demand
ISBN: 9535106953
Category : Technology & Engineering
Languages : en
Pages : 418
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Book Description
This book conveys many significant messages for the food engineering and allied professions: the importance of working in multidisciplinary teams, the relevance of developing food engineering based on well-established principles, the benefits of developing the field by bringing together experts from industry, academia and government, and the unparalleled advantage of working as globally as possible in the understanding, development, and applications of food engineering principles. I am delighted to welcome this book to the Series and I am convinced colleagues from all parts of the world will gain great value from it.
