Improved Methods for Characterization of Protein Dynamics by NMR SpectroscopyÃÂ and Studies of the EphB2 Kinase Domain PDF Download
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Author: Alexandra Ahlner
Publisher:
ISBN:
Category : Electronic book
Languages : en
Pages :
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Author: Alexandra Ahlner
Publisher:
ISBN:
Category : Electronic book
Languages : en
Pages :
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Author: Oleg Jardetzky
Publisher: Springer Science & Business Media
ISBN: 1461548950
Category : Science
Languages : en
Pages : 227
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This volume is a collection of articles from the proceedings of the International School of Structural Biology and Magnetic Resonance 3rd Course: Protein Dynamics, Function, and Design. This NATO Advance Study Institute was held in Erice at the Ettore Majorana Centre for Scientific Culture on April 16-28, 1997. The aim of the Institute was to bring together experts applyipg different physical methods to problems of macro molecular dynamics-notably x-ray diffraction, NMR and other forms of spectroscopy, and molecular dynamics simulations. Emphasis was placed on those systems and types of problems-such as mechanisms of allosteric control, signal transmission, induced fit to different ligands with its implications for drug design, and the effects of dynamics on structure determination-where a correlation of findings obtained by different methods could shed the most light on the mechanisms involved and stimulate the search for new approaches. The individual articles represent the state of the art in each of the areas cov ered and provide a guide to the original literature in this rapidly developing field. v CONTENTS 1. Determining Structures of ProteinlDN A Complexes by NMR Angela M. Gronenbom and G. Marius Clore 2. Fitting Protein Structures to Experimental Data: Lessons from before Your Mother Was Born . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Jeffrey C. Hoch, Alan S. Stem, and Peter J. Connolly 3. Multisubunit Allosteric Proteins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 William N. Lipscomb 4. Studying Protein Structure and Function by Directed Evolution: Examples with Engineered Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Andreas Pliickthun 5. High Pressure Effects on Protein Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Author: Vladimir N. Uversky
Publisher: Nova Publishers
ISBN: 9781600217036
Category : Science
Languages : en
Pages : 326
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Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
Author:
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Category :
Languages : en
Pages :
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Author: Dennis R. Livesay
Publisher: Humana
ISBN: 9781493963072
Category : Science
Languages : en
Pages : 0
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In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Dynamics: Methods and Protocols describes the most common and powerful methods used to characterize protein dynamics.
Author: Youqin Li
Publisher:
ISBN:
Category :
Languages : en
Pages : 494
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Author: Tai Te Wu
Publisher: Van Nostrand Reinhold Company
ISBN:
Category : Science
Languages : en
Pages : 216
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Author: Megan C. Thielges
Publisher: ProQuest
ISBN: 9781109124897
Category : Cytochrome c
Languages : en
Pages : 464
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Book Description
Fluctuations in protein structure on a broad range of time scales contribute to protein function. Infrared (IR) and visible spectroscopy are well suited for the study of fast processes, and accordingly, extensive effort has been aimed toward the development of these techniques for studying protein dynamics. Unfortunately, the use of IR spectroscopy for protein studies is limited by the availability of probes for characterizing specific regions of proteins. We have been developing the use of carbon deuterium (C-D) bonds as IR probes of protein electrostatics and dynamics. The isolated frequency of C-D bonds enables their observation on the conjested background of a protein IR spectra, and introduction of C-D results in a virtually native protein. This thesis aims to develop C-D bonds as probes of proteins by incorporation and characterization of C-D bonds in ligand complexes of the enzyme dihydrofolate reductase. In addition, C-D bonds were applied toward understanding the folding of evolutionarily related cytochromes c (cyt c). In effort toward extending the technique to time-resolved studies, we initiated experiments to monitor the CO rebinding to cyt c after photolysis with step scan FTIR spectroscopy. Finally, the second aim of this thesis is the application of current nonlinear spectroscopic experiments toward novel biological questions. Specifically, we report the application of 3PEPS and transient grating spectroscopies to study the impact of sequence diversity on the dynamics of set of thermodynamically diverse antibody-fluorescein complexes. We find that the dynamics of the Ab are diverse, and observe rough correlations between conformational heterogeneity and both binding entropy and the number of somatic mutations introduced during affinity maturation.
Author: Isao Suetake
Publisher: Wiley-Blackwell
ISBN: 9781119886358
Category : Science
Languages : en
Pages : 0
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"Proteins are the building blocks of living organisms, and they play an enormous range of fundamental roles in sustaining and shaping life. The critical determinant of a protein's function is its structure, and the analysis of protein structures has therefore become a significant component of biological research. In recent years, longstanding analytical techniques such as X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy have been supplemented by a number of new methods which promise to revolutionize the study of proteins and their functions. Analytical Techniques for the Elucidation of Protein Function serves as an introduction to these techniques, which are especially crucial for analyzing intrinsically disordered regions and post-translational modifications. These have revolutionized the study of proteins in recent years, and conventional methods for analyzing protein structures are no longer sufficient to work through their ramifications. This book therefore brings greater awareness of techniques which promise to produce the very cutting edge of protein research"--
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ISBN:
Category :
Languages : en
Pages : 200
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