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Author: R. Edwards
Publisher: OUP Oxford
ISBN: 0191565563
Category : Science
Languages : en
Pages : 302
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Book Description
Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostic tests are also used in other fields such as forensic science and environmental and food analysis. The different types of test range from simple manual methods to fully automated systems with sophisticated integrated detection. Immunodiagnostics: A Practical Approach starts off by explaining the principles and development of immunodiagnostic tests, specifically the use of radioisotopes as tracers. Chapter 2 explains the use of solid-phase supports to bind immunoreagents. Enzymes are widely used as labels in immunoassays and their use with colourimetric, fluorimetric, and chemiluminescent detection systems is described. The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non- radioisotpoc prodcedures is the time-resolved fluorescence assay. Enzymes can also be used as a simple method of obtaining high performance from immunodiagnostics and this application is covered later in the book. The next set of techniques to be described are light scattering techniques, which can be used in either simple manual assays or in sophisticated automated procedures. The penultimate chapter describes the principles of automation of immunodiagnostic tests. The last topic to be discussed is that of quality assurance.
Author: R. Edwards
Publisher: OUP Oxford
ISBN: 0191565563
Category : Science
Languages : en
Pages : 302
Get Book Here
Book Description
Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostic tests are also used in other fields such as forensic science and environmental and food analysis. The different types of test range from simple manual methods to fully automated systems with sophisticated integrated detection. Immunodiagnostics: A Practical Approach starts off by explaining the principles and development of immunodiagnostic tests, specifically the use of radioisotopes as tracers. Chapter 2 explains the use of solid-phase supports to bind immunoreagents. Enzymes are widely used as labels in immunoassays and their use with colourimetric, fluorimetric, and chemiluminescent detection systems is described. The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non- radioisotpoc prodcedures is the time-resolved fluorescence assay. Enzymes can also be used as a simple method of obtaining high performance from immunodiagnostics and this application is covered later in the book. The next set of techniques to be described are light scattering techniques, which can be used in either simple manual assays or in sophisticated automated procedures. The penultimate chapter describes the principles of automation of immunodiagnostic tests. The last topic to be discussed is that of quality assurance.
Author: Ian C. Clift
Publisher: Jones & Bartlett Learning
ISBN: 1284219259
Category : Medical
Languages : en
Pages : 770
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Book Description
A contemporary guide to the diagnostic principles and practices of immunology and serology in the clinical laboratory.
Author: Ralph Michael Aloisi
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 312
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Book Description
Author: David Wild
Publisher: Newnes
ISBN: 0080970389
Category : Science
Languages : en
Pages : 1037
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Book Description
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Author: Sandeep K. Vashist
Publisher: Academic Press
ISBN: 012811794X
Category : Medical
Languages : en
Pages : 498
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Book Description
Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays
Author: Ronald A. Rader
Publisher:
ISBN: 9780963957306
Category : Science
Languages : en
Pages : 682
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Book Description
"...designed to provide the U.S. biotechnology and pharmaceutical industries and the biomedical and life sciences research communities with access to federal technology transfer opportunities and activities."--Pref.
Author: Krystyna Sztefko
Publisher: Walter de Gruyter
ISBN: 3110249472
Category : Medical
Languages : en
Pages : 177
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Book Description
Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Proper validation of methods and quality assurance have little effect on frequency of occurrence of false positive or false negative results, which, if unrecognized, may lead to patient's misdiagnosis, unnecessary treatment or even unnecessary surgery. Deep knowledge of basic principles of immunochemical methods (antigen-antibody reaction, standardization, matrix effect, limit of detection, cross-reactivity, etc.), sources of analyte-independent interferences (preanalitycal errors, the presence of binding proteins, the presence of autoantibodies) and analyte-dependent interferences (presence of heterophilic antibodies, high-dose effect) are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results and, at the same time, improve patient's care and patient's safety.
Author: Carol A. Clausen
Publisher:
ISBN:
Category : Engineered wood
Languages : en
Pages : 12
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Book Description
Author: Pankaj Suman
Publisher: Springer Nature
ISBN: 981155823X
Category : Medical
Languages : en
Pages : 247
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Book Description
This book presents the timeline of immunodiagnostics evolution, including advancements in immunological/nucleic acid probes, assay design, labelling techniques, and devices for signal transduction and acquisition. In the past few years, enzyme and nanocatalyst-based immune assays have undergone numerous modifications to enhance their sensitivity and potential for automation. Further, to reduce production costs and the use of laboratory animals, engineering small antibodies and nucleic acid probes (aptamers) has become increasingly popular in the development of novel and powerful bioassays. In light of the notable advancements in immunodiagnostics, this book highlights the combined efforts of clinicians, biotechnologists, material scientists, nanotechnologists and basic scientists in a coherent and highly structured way. The book takes readers on the journey of immunodiagnostic technologies, from their introduction to the present.
Author: S.C. Rastogi
Publisher: Taylor & Francis
ISBN: 9788122409086
Category : Clinical immunology
Languages : en
Pages : 160
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Book Description
S.C. Rastogi, Formerly Professor Of Biological Sciences, B.I.T.S., Pilani, Has Vast Experience Of Teaching And Research Spanning More Than 40 Years. After His Training In Molecular Biology At Tata Institute Of Fundamental Research (Tifr), Mumbai, He Worked Constantly To Modernise Biology Courses At Bits. He Has Taught And Supervised Doctoral Research In Computer Applications In Molecular Biology, Enzyme Biotechnology And Physiology, And Has Successfully Completed Several Research Projects. He Has Edited Many Proceedings Of Scientific Symposia And Authored Research Papers And Books In The Field Of Bioinformatics, Biochemistry, Physiology, Molecular Biology And Biotechnology. As A Biologist Of Repute, And Accredited With Many Educational Innovations, He Has Been A Constant Participant In Course Development Work At Bits And Otheruniversities.
