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Author: Tina Thi My Tien Tran
Publisher:
ISBN:
Category : Ethanol
Languages : en
Pages :
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Book Description
S. cerevisiae has evolved the ability to tolerate high concentrations of ethanol, a trait that has contributed to this yeast being a cornerstone of beverage and biofuel industries. The key genes involved in conferring ethanol tolerance in S. cerevisiae are unknown. One strategy used to identify genes conferring ethanol tolerance, was to create ethanol-tolerant (ET) mutants from a laboratory yeast strain and use these to identify ethanol-tolerance conferring loci. Dr. Dragana Stanley (Victoria University) created two ET mutants, a spontaneous (SM) and a chemical (CM) mutant from W303, via adaptive evolution (Stanley 2008). Transcriptome analysis of the resultant ET mutants found that expression levels of hundreds of genes were altered, relative to the parent under ethanol-stress conditions. The primary objective of this thesis was to identify genes that confer ethanol tolerance in SM and CM.
Author: Tina Thi My Tien Tran
Publisher:
ISBN:
Category : Ethanol
Languages : en
Pages :
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Book Description
S. cerevisiae has evolved the ability to tolerate high concentrations of ethanol, a trait that has contributed to this yeast being a cornerstone of beverage and biofuel industries. The key genes involved in conferring ethanol tolerance in S. cerevisiae are unknown. One strategy used to identify genes conferring ethanol tolerance, was to create ethanol-tolerant (ET) mutants from a laboratory yeast strain and use these to identify ethanol-tolerance conferring loci. Dr. Dragana Stanley (Victoria University) created two ET mutants, a spontaneous (SM) and a chemical (CM) mutant from W303, via adaptive evolution (Stanley 2008). Transcriptome analysis of the resultant ET mutants found that expression levels of hundreds of genes were altered, relative to the parent under ethanol-stress conditions. The primary objective of this thesis was to identify genes that confer ethanol tolerance in SM and CM.
Author: Sylvie Callegari
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 152
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Book Description
Saccharomyces cerevisiae express a high degree of ethanol tolerance compared to other microorganisms. The complete mechanism involved in this tolerance has not yet been fully identified, but is likely to have evolved during naturally occurring fermentations in the wild. Microarray data has revealed a total of 24 genes up-related greater than three-fold in cells that have adapted to 5-6% ethanol. These genes may potentially have a role in the ethanol stress mechanisms of S. cerevisiae; however, previous studies examining genome/phenome relationship under stress conditions have failed to establish a correlation between gene up-regulation and function. To analyse the genome/phenome relationship for ethanol stress, deletion mutants were obtained and confirmed by PCR for several of the genes identified in the microarray data. They were then assayed using a series of ethanol assays differing in range and sensitivity. Nine of the ten deletion mutants assayed revealed an ethanol susceptible phenotype and encode proteins with a range of functions including wall synthesis, protein stabilisation, maintenance of ionic homeostasis and response to nutrient starvation. These are believed to have a role in combating ethanol stress. Results have also given an insight into the complexity of the S. cerevisiae mechanisms of ethanol tolerance, revealing that despite being commonly up-regulated, the genes have very diverse roles in which phenotypes are condition dependant. Contrary to previous studies a genome/phenome relationship has been detected for a great majority of the ethanol induced genes assayed, signifying the value of a comprehensive and sensitive system for the functional analysis of genes.
Author: Renko Goyashiki
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
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Book Description
Author: Michael James Anderson
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Amparo Querol
Publisher: Springer Science & Business Media
ISBN: 3540283986
Category : Science
Languages : en
Pages : 457
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Book Description
As a group of microorganisms, yeasts have an enormous impact on food and bev- age production. Scientific and technological understanding of their roles in this p- duction began to emerge in the mid-1800s, starting with the pioneering studies of Pasteur in France and Hansen in Denmark on the microbiology of beer and wine fermentations. Since that time, researchers throughout the world have been engaged in a fascinating journey of discovery and development – learning about the great diversity of food and beverage commodities that are produced or impacted by yeast activity, about the diversity of yeast species associated with these activities, and about the diversity of biochemical, physiological and molecular mechanisms that underpin the many roles of yeasts in food and beverage production. Many excellent books have now been published on yeasts in food and beverage production, and it is reasonable to ask the question – why another book? There are two different approaches to describe and understand the role of yeasts in food and beverage production. One approach is to focus on the commodity and the technology of its processing (e. g. wine fermentation, fermentation of bakery products), and this is the direction that most books on food and beverage yeasts have taken, to date. A second approach is to focus on the yeasts, themselves, and their bi- ogy in the context of food and beverage habitats.
Author: Friedrich K. Zimmermann
Publisher: CRC Press
ISBN: 9781566764667
Category : Technology & Engineering
Languages : en
Pages : 590
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Book Description
Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.
Author: Anthony John Heinrich
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 292
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Book Description
"The yeast Saccharomyces cerevisiae is used in many industrial applications including beer brewing, bread making, and winemaking. Winemaking yeast strains have the ability to convert grape sugars into alcohol and other metabolites consistent with good wine. An exploratory comparative approach was undertaken to identify the genes and corresponding proteins that give wine yeast strains of S. cerevisiae their distinctive phenotype, with a focus on studying genes that provide tolerance to ethanol." --p. ii.
Author: Andriy Sibirny
Publisher: Springer
ISBN: 303021110X
Category : Science
Languages : en
Pages : 568
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Book Description
This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Author: Stefan Hohmann
Publisher: Springer Science & Business Media
ISBN: 3540456112
Category : Science
Languages : en
Pages : 398
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Book Description
Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.
Author: Antonio Morata
Publisher: BoD – Books on Demand
ISBN: 9535135996
Category : Technology & Engineering
Languages : en
Pages : 302
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Book Description
Yeast - Industrial Applications is a book that covers applications and utilities of yeasts in food, chemical, energy, and environmental industries collected in 12 chapters. The use of yeasts in the production of metabolites, enzymatic applications, fermented foods, microorganism controls, bioethanol production, and bioremediation of contaminated environments is covered showing results, methodologies, and processes and describing the specific role of yeasts in them. The traditional yeast Saccharomyces cerevisiae is complemented in many applications with the use of less known non-Saccharomyces yeasts that now are being used extensively in industry. This book compiles the experience and know-how of researchers and professors from international universities and research centers.
