Identification and Characterization of Factors Involved in the Nonsense-mediated MRNA Decay Pathway PDF Download
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Author: Ying Cui
Publisher:
ISBN:
Category :
Languages : en
Pages : 422
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Book Description
Author: Ying Cui
Publisher:
ISBN:
Category :
Languages : en
Pages : 422
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Book Description
Author: Jonathan Philip Belk
Publisher:
ISBN:
Category :
Languages : en
Pages : 370
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Book Description
Author: Lynne E. Maquat
Publisher: CRC Press
ISBN: 1498713394
Category : Science
Languages : en
Pages : 277
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Book Description
Nonsense-Mediated mRNA Decay is the first book devoted to nonsense-mediated mRNA decay (NMD). The rationale for such a book is the enormous information that studies of NMD have provided on the intricacies of post-transcriptional gene expression. The first five sections of the book are divided according to organism and begin with chapters on S. cere
Author: Lynne E. Maquat
Publisher: Academic Press
ISBN: 0080922074
Category : Science
Languages : en
Pages : 661
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Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
MRNAs containing premature translation termination codons (PTCs) are usually degraded by the nonsense-mediated mRNA decay (NMD) pathway. The mechanism of NMD in mammals is largely unknown. The cytoplasmic protein Hupf1 was the only trans-acting factor of NMD in mammals that was known at the beginning of this study. In order to identify and characterize proteins that are required for NMD in mammals, Hupf1-containing protein complexes were affinity purified from HeLa cells that stably express recombinant, double affinity tagged Hupf1-2tag at physiological levels. The analysis of these purified complexes by mass spectrometry revealed the presence of the proteins Hupf2 and Poly(A)-binding protein 1 (Pabp1). Using size exclusion chromatography it was shown that the majority of Hupf1 occurs as a monomer, but two complexes of distinct composition could be isolated. Further analysis of affinity purified Hupf1-containing complexes by Western Blot revealed that the presence of intact RNA stabilizes the interaction of Hupf1 with Hupf2 as well as the interaction of Hupf1 with the recently identified NMD factors Hupf3a and Hupf3b. The interaction between Pabp1 and Hupf1 was demonstrated to be strictly RNA-dependent. Even though the components of the exon-exon-junction complex (EJC) or the translational release factor eRF3 have been implicated in NMD in mammals, these proteins could not be found in the purified Hupf1-containing complexes, indicating that these proteins interact with Hupf1 transiently or not at all. The results described in this thesis indicate that Hupf1 occurs in different stable protein complexes and that Hupf2 and Pabp1 are novel interaction partners of Hupf1 and might be involved in NMD in mammals. RNA was found to play an important role for the stability of the interactions of Hupf1 with other proteins. The dynamic interactions of Hupf1 are likely to play an important role for the recognition of PTC-containing mRNAs and their subsequent destabilization.
Author: Lynne Maquat
Publisher: Academic Press
ISBN: 9780123745842
Category : Medical
Languages : en
Pages : 417
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Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. * Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 222
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Book Description
Author: Manuel Selg
Publisher:
ISBN:
Category :
Languages : en
Pages : 450
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Book Description
Author: Peter L. Stern
Publisher: Cambridge University Press
ISBN: 9780521622639
Category : Medical
Languages : en
Pages : 304
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Book Description
Rapid progress in the definition of tumor antigens, and improved immunization methods, bring effective cancer vaccines within reach. In this wide-ranging survey, leading clinicians and scientists review therapeutic cancer vaccine strategies against a variety of diseases and molecular targets. Intended for an interdisciplinary readership, their contributions cover the rationale, development, and implementation of vaccines in human cancer treatment, with specific reference to cancer of the cervix, breast, colon, bladder, and prostate, and to melanoma and lymphoma. They review target identification, delivery vectors and clinical trial design. The book begins and ends with lucid overviews from the editors, that discuss the most recent developments.
Author: Nahum Sonenberg
Publisher: CSHL Press
ISBN: 9780879696184
Category : Gene expression
Languages : en
Pages : 1034
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Book Description
Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.
