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Author: Milton T. W. Hearn
Publisher: Wiley-VCH
ISBN: 9780895732958
Category : ADN - Séparation
Languages : en
Pages : 776
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Book Description
Author: Milton T. W. Hearn
Publisher: Wiley-VCH
ISBN: 9780895732958
Category : ADN - Séparation
Languages : en
Pages : 776
Get Book Here
Book Description
Author: M. T. W. Hearn
Publisher: Wiley-VCH
ISBN: 9780471187165
Category : Science
Languages : en
Pages : 776
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Book Description
A detailed examination of contemporary topics and applications of HPLC and related techniques in the analysis and purification of biomolecules. Major topics treated include: * sample and column preparation * column materials * protein interactions * preparative and purification chromatography * multimode and microscale chromatography * affinity chromatography * high performance electrophoresis This book is the second volume in the continuing series 'Analytical Techniques in Clinical Chemistry and Laboratory Medicine'.
Author: International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides (6, 1986, Baden-Baden)
Publisher:
ISBN:
Category :
Languages : en
Pages : 448
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Book Description
Author: Karen M. Gooding
Publisher: CRC Press
ISBN: 0203911598
Category : Science
Languages : en
Pages : 791
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Book Description
Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
Author: F.E. Regnier
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: K. Unger
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Milton Hearn
Publisher: Elsevier
ISBN: 032315770X
Category : Science
Languages : en
Pages : 284
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Book Description
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.
Author:
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Category :
Languages : en
Pages :
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Author:
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ISBN:
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Languages : en
Pages :
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Author: Marie-Isabel Aguilar
Publisher: Springer Science & Business Media
ISBN: 1592597424
Category : Science
Languages : en
Pages : 411
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Book Description
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
