High-performance Liquid Chromatography: Bonded silica phases for the separations of biopolymers by means of column liquid chromatography PDF Download
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Author: Csaba Horváth
Publisher:
ISBN: 9780123122056
Category : Liquid chromatography
Languages : en
Pages :
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Book Description
Author: Csaba Horváth
Publisher:
ISBN: 9780123122056
Category : Liquid chromatography
Languages : en
Pages :
Get Book Here
Book Description
Author: Csaba Horváth
Publisher: Elsevier
ISBN: 1483261840
Category : Science
Languages : en
Pages : 342
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Book Description
High-Performance Liquid Chromatography: Advances and Perspectives, Volume 5 presents the applications of high-performance liquid chromatography to the analysis and purification of biopolymers. The book, composed of three chapters, provides a detailed description of silica gel-supported stationary phases; tackles biospecific interaction chromatography, a tool in the study of complex carbohydrates and as an industrial separation process in biotechnology; and discusses the potential advantages of displacement chromatography for multicomponent separations. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good source of information.
Author: Elena Katz
Publisher: John Wiley & Sons
ISBN:
Category : Science
Languages : en
Pages : 544
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Book Description
This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Author: K.K. Unger
Publisher: Elsevier Inc. Chapters
ISBN: 0128071303
Category : Science
Languages : en
Pages : 69
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Book Description
This chapter deals with the most important part of the LC system: the column enabling the efficient and fast resolution of complex mixtures. It is divided into seven sections under the overall aspects of manufacture, operation, and evaluation of analytical columns for the user. The first three sections highlight the column design and hardware, followed by a thorough treatment of the properties of microparticulate silica adsorbents as packing material, stationary phases performed by surface functionalization, and the column filling process. Then, the implementation of the column into the LC system is discussed, leading into chromatographic column testing as a C18 bonded reversed silica column and its maintenance. Section 3.7 is devoted to today’s column market, focusing on the three main types of silica columns: fully porous microparticulate silica columns , columns with core–shell silica particles, and monolithic silica columns. Within a period of 40 years, analytical HPLC has generated high-resolution, robust, and rugged reversed-phase silica columns, which enable efficient, highly sensitive, and fast separations of low-molecular-weight substances based on validated methods.
Author: Danilo Corradini
Publisher: CRC Press
ISBN: 1420016946
Category : Science
Languages : en
Pages : 715
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Book Description
High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
Author: Ed: Elena D. Katz
Publisher: John Wiley & Sons
ISBN: 9788126519019
Category : Biotechnology
Languages : en
Pages : 542
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Book Description
This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Author: Ronald William Alfred Oliver
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 256
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Book Description
Author: William S. Hancock
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 580
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Book Description
High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock This important book details the broad range of analytical techniques that have made HPLC so successful in the separation and quantitation of product variants-a procedure essential to quality assurance of protein pharmaceuticals. Outlined are the major chromatographic techniques-reversed phase, ion exchange, affinity and hydrophobic interaction chromatography-as well as their role in specific separations. Also described are the methods required for a quality control program. Reflecting the heightened concern for product integrity since the advent of new protein pharmaceuticals by recombinant-DNA techniques, this critical reference will enhance the libraries of analytical chemists, biochemists, and chemical engineers working with HPLC. 1990 (0-471-82584-0) 624 pp.
Author: M.V. Novotny
Publisher: Elsevier
ISBN: 0080858333
Category : Science
Languages : en
Pages : 349
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Book Description
Edited by two of the pioneers of microcolumn chromatography and written by recognized experts in the field, this book summarizes advances in microcolumn liquid chromatography, capillary supercritical fluid chromatography and microelectrophoresis. Its unique combination of expert knowledge from leading laboratories in the USA, Japan and Switzerland, results in a particularly in-depth and comprehensive coverage of the various aspects of microcolumn separation methods.
Author: Karen M. Gooding
Publisher: CRC Press
ISBN: 0824755057
Category : Science
Languages : en
Pages : 763
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Book Description
Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
