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Author: Serghei Mangul
Publisher: Springer
ISBN: 3030139735
Category : Computers
Languages : en
Pages : 99
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Book Description
Advances in RNA-sequencing (RNA-seq) technologies have provided an unprecedented opportunity to explore the gene expression landscape across individuals, tissues, and environments by efficiently profiling the RNA sequences present in the samples. When a reference genome sequence or a transcriptome of the sample is available, mapping-based RNA-seq analysis protocols align the RNA-seq reads to the reference sequences, identify novel transcripts, and quantify the abundance of expressed transcripts.The reads that fail to map to the human reference, known as unmapped reads, are a large and often overlooked output of standard RNA-seq analyses. Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled. Reads can also remain unmapped due to unknown transcripts, recombined B and T cell receptor sequences, A-to-G mismatches from A-to-I RNA editing, trans-splicing, gene fusion, circular RNAs, and the presence of non-host RNA sequences (e.g. bacterial, fungal, and viral organisms). Unmapped reads represent a rich resource for the study of B and T cell receptor repertoires and the human microbiome system—without incurring the expense of additional targeted sequencing.This book introduces and describes the Read Origin Protocol (ROP), a tool that identifies the origin of both mapped and unmapped reads. The protocol first identifies human reads using a standard high-throughput algorithm to map them onto a reference genome and transcriptome. After alignment, reads are grouped into genomic (e.g. CDS, UTRs, introns) and repetitive (e.g. SINEs, LINEs, LTRs) categories. The rest of the ROP protocol characterizes the remaining unmapped reads, which failed to map to the human reference sequences.
Author: Serghei Mangul
Publisher: Springer
ISBN: 3030139735
Category : Computers
Languages : en
Pages : 99
Get Book Here
Book Description
Advances in RNA-sequencing (RNA-seq) technologies have provided an unprecedented opportunity to explore the gene expression landscape across individuals, tissues, and environments by efficiently profiling the RNA sequences present in the samples. When a reference genome sequence or a transcriptome of the sample is available, mapping-based RNA-seq analysis protocols align the RNA-seq reads to the reference sequences, identify novel transcripts, and quantify the abundance of expressed transcripts.The reads that fail to map to the human reference, known as unmapped reads, are a large and often overlooked output of standard RNA-seq analyses. Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled. Reads can also remain unmapped due to unknown transcripts, recombined B and T cell receptor sequences, A-to-G mismatches from A-to-I RNA editing, trans-splicing, gene fusion, circular RNAs, and the presence of non-host RNA sequences (e.g. bacterial, fungal, and viral organisms). Unmapped reads represent a rich resource for the study of B and T cell receptor repertoires and the human microbiome system—without incurring the expense of additional targeted sequencing.This book introduces and describes the Read Origin Protocol (ROP), a tool that identifies the origin of both mapped and unmapped reads. The protocol first identifies human reads using a standard high-throughput algorithm to map them onto a reference genome and transcriptome. After alignment, reads are grouped into genomic (e.g. CDS, UTRs, introns) and repetitive (e.g. SINEs, LINEs, LTRs) categories. The rest of the ROP protocol characterizes the remaining unmapped reads, which failed to map to the human reference sequences.
Author: Serghei Mangul
Publisher:
ISBN: 9783030139742
Category : Nucleotide sequence
Languages : en
Pages : 93
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Book Description
Advances in RNA-sequencing (RNA-seq) technologies have provided an unprecedented opportunity to explore the gene expression landscape across individuals, tissues, and environments by efficiently profiling the RNA sequences present in the samples. When a reference genome sequence or a transcriptome of the sample is available, mapping-based RNA-seq analysis protocols align the RNA-seq reads to the reference sequences, identify novel transcripts, and quantify the abundance of expressed transcripts. The reads that fail to map to the human reference, known as unmapped reads, are a large and often overlooked output of standard RNA-seq analyses. Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled. Reads can also remain unmapped due to unknown transcripts, recombined B and T cell receptor sequences, A-to-G mismatches from A-to-I RNA editing, trans-splicing, gene fusion, circular RNAs, and the presence of non-host RNA sequences (e.g. bacterial, fungal, and viral organisms). Unmapped reads represent a rich resource for the study of B and T cell receptor repertoires and the human microbiome system--without incurring the expense of additional targeted sequencing. This book introduces and describes the Read Origin Protocol (ROP), a tool that identifies the origin of both mapped and unmapped reads. The protocol first identifies human reads using a standard high-throughput algorithm to map them onto a reference genome and transcriptome. After alignment, reads are grouped into genomic (e.g. CDS, UTRs, introns) and repetitive (e.g. SINEs, LINEs, LTRs) categories. The rest of the ROP protocol characterizes the remaining unmapped reads, which failed to map to the human reference sequences.
Author: Fabio Marchi
Publisher: BoD – Books on Demand
ISBN: 9535135031
Category : Medical
Languages : en
Pages : 330
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Book Description
The large potential of RNA sequencing and other "omics" techniques has contributed to the production of a huge amount of data pursuing to answer many different questions that surround the science's great unknowns. This book presents an overview about powerful and cost-efficient methods for a comprehensive analysis of RNA-Seq data, introducing and revising advanced concepts in data analysis using the most current algorithms. A holistic view about the entire context where transcriptome is inserted is also discussed here encompassing biological areas with remarkable technological advances in the study of systems biology, from microorganisms to precision medicine.
Author: Dóra Szakonyi
Publisher: Frontiers Media SA
ISBN: 2889630250
Category :
Languages : en
Pages : 167
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Book Description
Discoveries from the past decades revealed that RNA molecules are much more than inert intermediates between the coding DNA sequences and their functional products, proteins. Today, RNAs are recognized as active regulatory molecules influencing gene expression, chromatin organization and genome stability, thus impacting all aspects of plant life including development, growth, reproduction and stress tolerance. Innovations in methodologies, the expanding application of next-generation sequencing technologies, and the creation of public datasets and databases have exposed a new universe of RNA-based mechanisms and led to the discovery of new families of non-coding RNAs, uncovered the large extent of alternative splicing events, and highlighted the potential roles of RNA modifications and RNA secondary structures. Furthermore, considerable advances have been made in identifying RNA-binding and processing factors involved in the synthesis and maturation of different forms of RNA molecules as well as in RNA processing, biochemical modifications or degradation. This Research Topic showcases the broad biological significance of RNAs in plant systems and contains eight original research articles, one review and four mini-reviews, covering various RNA-based mechanisms in higher plants. Emerging new technologies and novel multidisciplinary approaches are empowering the scientific community and will expectedly bring novel insights into our understanding of the mechanisms through which RNA is regulated and regulates biological processes in plant cells.
Author: Jose Victor Lopez
Publisher: Elsevier
ISBN: 0128241136
Category : Science
Languages : en
Pages : 264
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Book Description
Assessments and Conservation of Biological Diversity from Coral Reefs to the Deep Sea: Uncovering Buried Treasures and the Value of the Benthos examines marine benthic habitats around the world that are linked by their physical location at the bottom of the oceans. The book approaches deep sea marine biodiversity with perspectives on genetics, microbiology and evolution, weaving a narrative of vital expert linkages with the goal of protecting something that most people cannot witness or experience. It provides a full assessment of biological diversity within benthic habitats, from coral reefs to plankton and fish species, and offers global case studies. It is the ideal resource for marine conservationists and biologists aiming to expand their knowledge and efforts to the rarely seen, yet equally important, realms of the ocean and respective benthic species. As these deep-sea ecosystems and their species face unprecedented threats of destruction and extinction due to factors including climate change, this book provides the most current knowledge of this undersea world along with solutions for its conservation. - Compares and contrasts between shallow and marine habitats to reveal revolutionary connections and continuity - Analyzes modern threats and gaps in biological knowledge regarding benthic communities - Examines benthic biodiversity through vertical vs. horizontal gradients - Poses possible solutions for the conservation of benthic habitats and organisms
Author: DR. AURORA MARTIN
Publisher: INTERDISCIPLINARY INSTITUTE OF HUMAN SECURITY & GOVERNANCE
ISBN: 9354575676
Category : Social Science
Languages : en
Pages : 402
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Book Description
The world turned topsy-turvy after Covid‑19 pandemic. The whole equation and thrust of global politics and economics is now at a new verge. The main question is now – how we can secure human race and humanity. This is the main thrust of the book, Changing Dimensions of Human Security in Contemporary World. This book is an outcome of collective research work done by erudite scholars from different parts of world, like USA, Russia, Japan, Australia, Romania, Nigeria, Nepal, Bangladesh and India, who has come together in search of new trends of human security. They touched upon different milieu as well as dimensions of human security and pandemic in contemporary world scenario. In thirty-two essays, forty-seven authors collectively explored situation of human security in several domains including international politics, law, economy, labour force, sustainable development, education, gender and may more. I believe that this collection of essays can become a benchmark for the future as well as spur new research agendas and projects that will put the region into a much-needed conversation on the recent trends of human security in contemporary world. This book will try to reimagining this changing dimension of Human Security in the Pandemic situation. The goal of this book is to improve the standards of the international community of academicians, researchers, scholars, and scientists by exposing them to the latest trends, developments, and challenges in the field. The volume is essential reading for social scientists, bureaucrats and non-governmental political activists interested in human security. It will also appeal to public policy analysts and scholars who have yet to adopt the contribution of critical security and development studies in the analysis of different dimensions of human security.
Author: K. Ramesh Reddy
Publisher: CRC Press
ISBN: 1498764568
Category : Science
Languages : en
Pages : 734
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Book Description
The globally important nature of wetland ecosystems has led to their increased protection and restoration as well as their use in engineered systems. Underpinning the beneficial functions of wetlands are a unique suite of physical, chemical, and biological processes that regulate elemental cycling in soils and the water column. This book provides an in-depth coverage of these wetland biogeochemical processes related to the cycling of macroelements including carbon, nitrogen, phosphorus, and sulfur, secondary and trace elements, and toxic organic compounds. In this synthesis, the authors combine more than 100 years of experience studying wetlands and biogeochemistry to look inside the black box of elemental transformations in wetland ecosystems. This new edition is updated throughout to include more topics and provide an integrated view of the coupled nature of biogeochemical cycles in wetland systems. The influence of the elemental cycles is discussed at a range of scales in the context of environmental change including climate, sea level rise, and water quality. Frequent examples of key methods and major case studies are also included to help the reader extend the basic theories for application in their own system. Some of the major topics discussed are: Flooded soil and sediment characteristics Aerobic-anaerobic interfaces Redox chemistry in flooded soil and sediment systems Anaerobic microbial metabolism Plant adaptations to reducing conditions Regulators of organic matter decomposition and accretion Major nutrient sources and sinks Greenhouse gas production and emission Elemental flux processes Remediation of contaminated soils and sediments Coupled C-N-P-S processes Consequences of environmental change in wetlands# The book provides the foundation for a basic understanding of key biogeochemical processes and its applications to solve real world problems. It is detailed, but also assists the reader with box inserts, artfully designed diagrams, and summary tables all supported by numerous current references. This book is an excellent resource for senior undergraduates and graduate students studying ecosystem biogeochemistry with a focus in wetlands and aquatic systems.
Author: Essam Halim Houssein
Publisher: Springer Nature
ISBN: 3030990796
Category : Technology & Engineering
Languages : en
Pages : 501
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Book Description
This book collects different methodologies that permit metaheuristics and machine learning to solve real-world problems. This book has exciting chapters that employ evolutionary and swarm optimization tools combined with machine learning techniques. The fields of applications are from distribution systems until medical diagnosis, and they are also included different surveys and literature reviews that will enrich the reader. Besides, cutting-edge methods such as neuroevolutionary and IoT implementations are presented in some chapters. In this sense, the book provides theory and practical content with novel machine learning and metaheuristic algorithms. The chapters were compiled using a scientific perspective. Accordingly, the book is primarily intended for undergraduate and postgraduate students of Science, Engineering, and Computational Mathematics and can be used in courses on Artificial Intelligence, Advanced Machine Learning, among others. Likewise, the material can be helpful for research from the evolutionary computation, artificial intelligence communities.
Author: Huanming Yang
Publisher: World Scientific
ISBN: 9814487171
Category : Science
Languages : en
Pages : 167
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Book Description
The aim of this book series is to familiarize scientists, students and the general public with exciting new discoveries and developments in rice genomics. Leading scientists in rice genomics and related fields are invited to write articles in a cohesive format that appeals to both researchers and laypersons with an interest in genomics and biotechnology. The first volume provides the background information and highlights the major achievements in rice genomics; ongoing developments in this exciting field are also discussed. This volume promotes genomics as a holistic approach to rice research and genetic engineering. The chief editor of the series is Prof Huanming Yang of the Beijing Genomics Institute. As one of the most distinguished scientists in rice research today, Prof Yang led a team of researchers who successfully sequenced and analyzed the draft sequence of the indica rice subspecies. In Vol 1 of the series, he co-authors an article entitled “An International Campaign for Agricultural and Livestock Genomics”.
Author: Yasha Hasija
Publisher: Academic Press
ISBN: 0128219734
Category : Medical
Languages : en
Pages : 453
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Book Description
Translational Biotechnology: A Journey from Laboratory to Clinics presents an integrative and multidisciplinary approach to biotechnology to help readers bridge the gaps between fundamental and functional research. The book provides state-of-the-art and integrative views of translational biotechnology by covering topics from basic concepts to novel methodologies. Topics discussed include biotechnology-based therapeutics, pathway and target discovery, biological therapeutic modalities, translational bioinformatics, and system and synthetic biology. Additional sections cover drug discovery, precision medicine and the socioeconomic impact of translational biotechnology. This book is valuable for bioinformaticians, biotechnologists, and members of the biomedical field who are interested in learning more about this promising field. - Explains biotechnology in a different light by using an application-oriented approach - Discusses practical approaches in the development of precision medicine tools, systems and dynamical medicine approaches - Promotes research in the field of biotechnology that is translational in nature, cost-effective and readily available to the community
