Author: Paul Wassarman
Publisher: Academic Press
ISBN: 0123848814
Category : Science
Languages : en
Pages : 637
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Book Description
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Author: Wassarman,Paul M
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Paul M. Wassarman
Publisher: Academic Press
ISBN: 9780123848833
Category : Medical
Languages : en
Pages : 482
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Book Description
17 years have passed since the first edition of the "Guide to Techniques in Mouse Development" (volume 225) was puolished. During this time development of technology used to investigate mouse development has not stood still, with enormous advances occurring in genomics, transgenic and ES cell methodology, and reprogramming, culminating with development of IPS cells. At both the cellular and molecular levels, a great many technological advances have been made that permit investigators to probe ever more deeply into all aspects of mouse development.
Author:
Publisher: Academic Press
ISBN: 0080887783
Category : Science
Languages : en
Pages : 539
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Book Description
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Author:
Publisher: Academic Press
ISBN: 9780123747754
Category : Science
Languages : en
Pages : 0
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Book Description
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.
Author:
Publisher: Academic Press
ISBN: 9780123747754
Category : Science
Languages : en
Pages : 536
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Book Description
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 Covers new technologies and methodologies, including: new techniques for the cryopreservation of gametes and embryos production of transgenic and null (knockout) animals (use of ES cells) generation of conditional/inducible mutant animals use of gene-trap mutagenesis analysis of allele-specific expresion use of new reporter constructs humanizing of transgenic animals transcript profiling of mouse development imaging of mouse development rederivation of animals and use of mouse genomics
Author: B. Anne Croy
Publisher: Academic Press
ISBN: 0123947944
Category : Science
Languages : en
Pages : 829
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Book Description
The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. - Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications - Describes the angiogenic tree the mother's uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes - Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation - Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success
Author: Paul M. Wassarman
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Amy Rosenzweig
Publisher: Academic Press
ISBN: 0123869064
Category : Science
Languages : en
Pages : 361
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Book Description
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. - Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements - The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
Author: P. Michael Conn
Publisher: Academic Press
ISBN: 012385928X
Category : Cell physiology
Languages : en
Pages : 466
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Book Description
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights
