Growing and Handling of Bacterial Cultures Within a Shared Core Facility for Integrated Structural Biology Program PDF Download
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Author: Julia Oxford
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 0
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Book Description
We have established and optimized standard operating procedures for growing and handling bacterial cultures in a shared core laboratory to support Integrative Structural Biology. The Integrative Structural Biology effort within the Biomolecular Research Center allows researchers to generate new knowledge about protein and RNA structure and function. We aim to understand how biomolecules assemble into stable structures and how structural dynamics impacts their function. Here we describe specific procedures for growing and handling bacterial cultures for overexpression and isolation of recombinant proteins, 15N/13C uniform labeling of recombinant proteins, protein isolation and purification, and analysis of protein solubility that are ideal for implementation in a shared research core laboratory that serves a multitude of diverse customers and research laboratories.
Author: Julia Oxford
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 0
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Book Description
We have established and optimized standard operating procedures for growing and handling bacterial cultures in a shared core laboratory to support Integrative Structural Biology. The Integrative Structural Biology effort within the Biomolecular Research Center allows researchers to generate new knowledge about protein and RNA structure and function. We aim to understand how biomolecules assemble into stable structures and how structural dynamics impacts their function. Here we describe specific procedures for growing and handling bacterial cultures for overexpression and isolation of recombinant proteins, 15N/13C uniform labeling of recombinant proteins, protein isolation and purification, and analysis of protein solubility that are ideal for implementation in a shared research core laboratory that serves a multitude of diverse customers and research laboratories.
Author: Madhusmita Mishra
Publisher:
ISBN: 1789858461
Category :
Languages : en
Pages : 154
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Book Description
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166802
Category : Science
Languages : en
Pages : 228
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Book Description
In the summer of 1803, Thomas Jefferson sent Meriwether Lewis and William Clark on a journey to establish an American presence in a land of unqualified natural resources and riches. Is it fitting that, on the 200th anniversary of that expedition, the United States, together with international partners, should embark on another journey of exploration in a vastly more extensive region of remarkable potential for discovery. Although the oceans cover more than 70 percent of our planet's surface, much of the ocean has been investigated in only a cursory sense, and many areas have not been investigated at all. Exploration of the Seas assesses the feasibility and potential value of implementing a major, coordinated, international program of ocean exploration and discovery. The study committee surveys national and international ocean programs and strategies for cooperation between governments, institutions, and ocean scientists and explorers, identifying strengths, weaknesses, and gaps in these activities. Based primarily on existing documents, the committee summarizes priority areas for ocean research and exploration and examines existing plans for advancing ocean exploration and knowledge.
Author: Christopher Somerville
Publisher: Fao
ISBN: 9789251085325
Category : Aquaponics
Languages : en
Pages : 0
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Book Description
This technical paper begins by introducing the concept of aquaponics, including a brief history of its development and its place within the larger category of soil-less culture and modern agriculture. It discusses the main theoretical concepts of aquaponics, including the nitrogen cycle and the nitrification process, the role of bacteria, and the concept of balancing an aquaponic unit. It then moves on to cover important considerations of water quality parameters, water testing, and water sourcing for aquaponics, as well as methods and theories of unit design, including the three main methods of aquaponic systems: media beds, nutrient film technique, and deep water culture. The publication discusses in detail the three groups of living organisms (bacteria, plants and fish) that make up the aquaponic ecosystem. It also presents management strategies and troubleshooting practices, as well as related topics, specifically highlighting local and sustainable sources of aquaponic inputs. The publication also includes nine appendixes that present other key topics: ideal conditions for common plants grown in aquaponics; chemical and biological controls of common pests and diseases including a compatible planting guide; common fish diseases and related symptoms, causes and remedies; tools to calculate the ammonia produced and biofiltration media required for a certain fish stocking density and amount of fish feed added; production of homemade fish feed; guidelines and considerations for establishing aquaponic units; a cost-benefit analysis of a small-scale, media bed aquaponic unit; a comprehensive guide to building small-scale versions of each of the three aquaponic methods; and a brief summary of this publication designed as a supplemental handout for outreach, extension and education.
Author: Franklin White
Publisher: Oxford University Press
ISBN: 0199876991
Category : Medical
Languages : en
Pages : 378
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Book Description
Amid ongoing shifts in the world economic and political order, the promise for future public health is tenuous. Will today's economic systems sustain tomorrow's health? Will future generations inherit fair access to health and health care? An important hope for the health of future generations is the establishment of a well-grounded, global public health system. Global Public Health: Ecological Foundations addresses both the challenges and cooperative solutions of contemporary public health, within a framework of social justice, environmental sustainability, and global cooperation. With an emphasis on ecological foundations, this book approaches public health principles-history, foundations, topics, and applications-with a community-oriented perspective. By achieving global reach through cooperative, community-based interventions, this text illustrates that the practical application of public health principles can help maintain the health of the world's people. Blending established wisdom with new perspectives, Global Public Health will stimulate better understanding of how the different streams of public health can work more synergistically to promote global health equity. It is a foundation for future public health measures to be built and to succeed.
Author: Nigel H. Banks
Publisher: Academic Press
ISBN: 0080920780
Category : Technology & Engineering
Languages : en
Pages : 637
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Book Description
Consideration of the interactions between decisions made at one point in the supply chain and its effects on the subsequent stages is the core concept of a systems approach. Postharvest Handling is unique in its application of this systems approach to the handling of fruits and vegetables, exploring multiple aspects of this important process through chapters written by experts from a variety of backgrounds. Newly updated and revised, this second edition includes coverage of the logistics of fresh produce from multiple perspectives, postharvest handing under varying weather conditions, quality control, changes in consumer eating habits and other factors key to successful postharvest handling. The ideal book for understanding the economic as well as physical impacts of postharvest handling decisions. Key Features: *Features contributions from leading experts providing a variety of perspectives *Updated with 12 new chapters *Focuses on application-based information for practical implementation *System approach is unique in the handling of fruits and vegetables
Author:
Publisher:
ISBN:
Category : Dental public health
Languages : en
Pages : 32
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Book Description
Author: Jancy C. McPhee
Publisher: U. S. National Aeronautics & Space Administration
ISBN:
Category : Biography & Autobiography
Languages : en
Pages : 396
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Book Description
Author: Leslie Neal-Boylan
Publisher: John Wiley & Sons
ISBN: 1118277856
Category : Medical
Languages : en
Pages : 432
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Book Description
Clinical Case Studies for the Family Nurse Practitioner is a key resource for advanced practice nurses and graduate students seeking to test their skills in assessing, diagnosing, and managing cases in family and primary care. Composed of more than 70 cases ranging from common to unique, the book compiles years of experience from experts in the field. It is organized chronologically, presenting cases from neonatal to geriatric care in a standard approach built on the SOAP format. This includes differential diagnosis and a series of critical thinking questions ideal for self-assessment or classroom use.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309154006
Category : Science
Languages : en
Pages : 246
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Book Description
A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.
