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Author: Martin Chalfie
Publisher: Wiley-Liss
ISBN:
Category : Science
Languages : en
Pages : 496
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Book Description
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Author: Martin Chalfie
Publisher: Wiley-Liss
ISBN:
Category : Science
Languages : en
Pages : 496
Get Book Here
Book Description
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Author: Martin Chalfie
Publisher: John Wiley & Sons
ISBN: 0471739480
Category : Science
Languages : en
Pages : 486
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Book Description
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Author: Osamu Shimomura
Publisher: World Scientific
ISBN: 9813202181
Category : Science
Languages : en
Pages : 229
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Book Description
This book narrates the uniquely intertwined life and scientific career of Nobel laureate Osamu Shimomura, with particular attention to his discovery of aequorin and Green Fluorescent Protein (GFP). It includes his early memories of Manchuria and wartime Japan featuring an eyewitness account of the atomic bombing of Nagasaki, his postwar studies and his travels to collect and research more than fifteen bioluminescent species, in locations ranging from Japan to San Juan Island, Bermuda, New Zealand and Norway. Dr Shimomura describes the unique combination of serendipity and perseverance that led ultimately to his 2008 Nobel Prize in chemistry. The book provides an engaging account of the life of a dedicated scientist, emphasizing the value of determination in the pursuit of pure scientific knowledge, and showing how a general understanding of science helped him open up new areas of research that have led to unforeseen applications in cell biology and medicine.
Author: Frank Delvigne
Publisher: Momentum Press
ISBN: 1606504177
Category : Technology & Engineering
Languages : en
Pages : 61
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Book Description
Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters. Recent developments in bioreactor technologies follow the scaling-out principle, i.e. carrying out several cultures in parallel with controlled conditions for screening purposes. Several mini-bioreactor concepts, i.e. bioreactor with working volume of 1 to 100 mL with controlling devices, have been developed following this principle. In general, chemical engineering similarities between conventional stirred bioreactors and their miniature equivalent are well characterized. However, the actual scaling-up rules are not able to cope with the complexity of the microbial stress response. Indeed, microbial stress response still remains not completely understood considering the process perturbations and the environmental fluctuations accompanying the scaling-up to industrial bioreactors. At this time, this kind of response can only be experimentally predicted by using scale-down bioreactors, i.e. lab-scale bioreactors designed in order to reproduce mixing imperfections that have to be expected at large-scale. However, the use of such an approach is time consuming and requires an experimented staff to elaborate the scaling-down protocols. Indeed, bioprocess development involves several steps which cannot be necessarily linked with each other considering the different cultivation equipment used.
Author: Gerald R. Fink
Publisher: Gulf Professional Publishing
ISBN: 9780121822545
Category : Science
Languages : en
Pages : 786
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Book Description
This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.
Author: Corinne A. Michels
Publisher: John Wiley & Sons
ISBN: 9780471899198
Category : Science
Languages : en
Pages : 264
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Book Description
Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.
Author: American Society for Cell Biology
Publisher: Gulf Professional Publishing
ISBN: 9780126760750
Category : Medical
Languages : en
Pages : 430
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Book Description
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features * Only volume on Green Fluorescent Protein research * Covers all aspects of GFP * Provides specific microscopic and data analysis methods * Discusses the design and construction of GFP fusion proteins * Covers GFP expression in animals, insects, plants, and microbes * Details procedures for time lapse imaging of living cells * Explains how to implement single molecule fluorescence detection with GFP * Discusses dual label GFP strategies for multicolor fluorescence * Presents fluorescence resonance energy transfer methods with GFPs * Details quantitative fluorescence imaging techniques * Extensively illustrated with color photographs
Author: Vincent Pieribone
Publisher: Harvard University Press
ISBN: 9780674019218
Category : Nature
Languages : en
Pages : 306
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Book Description
The discovery of green fluorescent protein revolutionized molecular biology, transforming our study of everything from the AIDS virus to the workings of the brain.
Author: Ron Milo
Publisher: Garland Science
ISBN: 1317230698
Category : Science
Languages : en
Pages : 400
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Book Description
A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Author: Krishnarao Appasani
Publisher: Cambridge University Press
ISBN: 9781139442657
Category : Science
Languages : en
Pages : 564
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Book Description
RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
