Genetic Variability of Maize (Zea Mays L.) Under Different Tissue Culture Conditions PDF Download
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Author: Manfred Beckert
Publisher:
ISBN:
Category :
Languages : en
Pages : 38
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Author: Manfred Beckert
Publisher:
ISBN:
Category :
Languages : en
Pages : 38
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Author: Y. P. S. Bajaj
Publisher: Springer Science & Business Media
ISBN: 364257968X
Category : Technology & Engineering
Languages : en
Pages : 644
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Book Description
Forty chapters deal with various aspects of tissue culture, in vitro manipulation, and other biotechnological approaches to the improvement of maize. They are arranged in eight sections: - In Vitro Technology, Callus Cultures and Regeneration of Plants, Somatic Embryogenesis. - Wide Hybridization, Embryo, Ovule, and Inflorescence Culture, in Vitro Fertilization. - Production of Haploids and Double Haploids, Anther and Pollen Culture. - Protoplast Culture, Genetic Transformation. - Somaclonal Variation and Mutations. - Molecular Biology and Physiological Studies. - Proteins and Nutritional Improvement. Pollen Storage, Cryopreservation of Germplasm.
Author: Robin K. Duffy Cowen
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
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Author: Frank Lloyd McFarland
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Maize is one of the most economically important crops in the US and globally for food, feed, and biofuel production. Efficient production of regenerable, embryogenic tissue cultures of maize is necessary for both basic biological research and applied biotechnological approaches to maize genetic improvement. Efficient generation of regenerable cultures in maize is highly genotype-dependent and, historically, researchers in academia and industry have relied extensively upon a restrictively small number of genotypes amenable to conventional tissue culture. To identify the genetic basis for genotypic differences in culture response, a mapping-based approach was utilized. The tissue culture response trait from the regenerable maize inbred A188 was backcrossed into the recalcitrant inbred B73. An A188 genomic introgression spanning 3 Mb on chromosome 3 was strongly associated with regenerable tissue culture response. Three candidate genes were selected in the region based on their expression in A188 immature embryos and functional annotations of genes in the region. A screen of each candidate gene expressed under constitutive promoters identified a single candidate, Wox2a, which was found to induce somatic embryogenesis and embryogenic callus in B73.A complementary approach to mapping the A188 culture response trait was undertaken to map the genotype-dependent response of diverse maize lines to Wuschel2/Baby boom (Wus/Bbm)-mediated genetic transformation. Though Wus/Bbm-mediated transformation reduced recalcitrance of many lines through co-transformation with developmental regulators, strong trends of genotype-dependence were still observed. Ten diverse maize lines were screened to identify lines highly amenable to Wus/Bbm-mediated transformation. Transgenic plants/events were recovered from eight of the ten lines. One line, Gaspé Flint, generated transgenic events efficiently and at a wide range of embryo maturities. A Gaspé Flint x B73 introgression library was screened through Wus/Bbm-mediated transformation and Gaspe̹ Flint introgressions associated with transformability were identified on chromosomes 1, 4, and 7. The scale of the transformation experiments necessitated isolation of thousands of immature embryo explants, for which a high-throughput method of embryo isolation (SIMPLE extraction) was developed. Methods for SIMPLE extraction, as well as optimized methods for Wus/Bbm-mediated and Wox2a-mediated maize transformation were developed to enable easy adoption these developing technologies.
Author: Ramakrishna Wusirika
Publisher: CRC Press
ISBN: 1482228130
Category : Science
Languages : en
Pages : 310
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Book Description
Sequencing of the maize genome has opened up new opportunities in maize breeding, genetics and genomics research. This book highlights modern trends in development of hybrids, analysis of genetic diversity, molecular breeding, comparative and functional genomics, epigenomicsand proteomics in maize. The use of maize in biofuels, phytoremediation and
Author: Kwanchai A. Gomez
Publisher: John Wiley & Sons
ISBN: 9780471870920
Category : Technology & Engineering
Languages : en
Pages : 698
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Book Description
Here in one easy-to-understand volume are the statistical procedures and techniques the agricultural researcher needs to know in order to design, implement, analyze, and interpret the results of most experiments with crops. Designed specifically for the non-statistician, this valuable guide focuses on the practical problems of the field researcher. Throughout, it emphasizes the use of statistics as a tool of research—one that will help pinpoint research problems and select remedial measures. Whenever possible, mathematical formulations and statistical jargon are avoided. Originally published by the International Rice Research Institute, this widely respected guide has been totally updated and much expanded in this Second Edition. It now features new chapters on the analysis of multi-observation data and experiments conducted over time and space. Also included is a chapter on experiments in farmers' fields, a subject of major concern in developing countries where agricultural research is commonly conducted outside experiment stations. Statistical Procedures for Agricultural Research, Second Edition will prove equally useful to students and professional researchers in all agricultural and biological disciplines. A wealth of examples of actual experiments help readers to choose the statistical method best suited for their needs, and enable even the most complicated procedures to be easily understood and directly applied. An International Rice Research Institute Book
Author: Frank Lloyd McFarland
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Maize is one of the most economically important crops in the US and globally for food, feed, and biofuel production. Efficient production of regenerable, embryogenic tissue cultures of maize is necessary for both basic biological research and applied biotechnological approaches to maize genetic improvement. Efficient generation of regenerable cultures in maize is highly genotype-dependent and, historically, researchers in academia and industry have relied extensively upon a restrictively small number of genotypes amenable to conventional tissue culture. To identify the genetic basis for genotypic differences in culture response, a mapping-based approach was utilized. The tissue culture response trait from the regenerable maize inbred A188 was backcrossed into the recalcitrant inbred B73. An A188 genomic introgression spanning 3 Mb on chromosome 3 was strongly associated with regenerable tissue culture response. Three candidate genes were selected in the region based on their expression in A188 immature embryos and functional annotations of genes in the region. A screen of each candidate gene expressed under constitutive promoters identified a single candidate, Wox2a, which was found to induce somatic embryogenesis and embryogenic callus in B73.A complementary approach to mapping the A188 culture response trait was undertaken to map the genotype-dependent response of diverse maize lines to Wuschel2/Baby boom (Wus/Bbm)-mediated genetic transformation. Though Wus/Bbm-mediated transformation reduced recalcitrance of many lines through co-transformation with developmental regulators, strong trends of genotype-dependence were still observed. Ten diverse maize lines were screened to identify lines highly amenable to Wus/Bbm-mediated transformation. Transgenic plants/events were recovered from eight of the ten lines. One line, Gaspé Flint, generated transgenic events efficiently and at a wide range of embryo maturities. A Gaspé Flint x B73 introgression library was screened through Wus/Bbm-mediated transformation and Gaspé Flint introgressions associated with transformability were identified on chromosomes 1, 4, and 7. The scale of the transformation experiments necessitated isolation of thousands of immature embryo explants, for which a high-throughput method of embryo isolation (SIMPLE extraction) was developed. Methods for SIMPLE extraction, as well as optimized methods for Wus/Bbm-mediated and Wox2a-mediated maize transformation were developed to enable easy adoption these developing technologies.
Author: V. Hemalatha
Publisher:
ISBN:
Category :
Languages : en
Pages : 114
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Author: Paul Frederick Umbeck
Publisher:
ISBN:
Category :
Languages : en
Pages : 220
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Author: Virginia Marie Peschke
Publisher:
ISBN:
Category :
Languages : en
Pages : 246
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