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Author: Yinghui Dan
Publisher: Bentham Science Publishers
ISBN: 1608052486
Category : Science
Languages : en
Pages : 154
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Book Description
"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "
Author: Yinghui Dan
Publisher: Bentham Science Publishers
ISBN: 1608052486
Category : Science
Languages : en
Pages : 154
Get Book Here
Book Description
"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "
Author: Hau-Hsuan Hwang
Publisher: Frontiers Media SA
ISBN: 2889195740
Category : Botany
Languages : en
Pages : 167
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Book Description
The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.
Author: Phetole Mangena
Publisher: CRC Press
ISBN: 1000638154
Category : Science
Languages : en
Pages : 244
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Book Description
Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.
Author: John Flex Jackson
Publisher: Springer Science & Business Media
ISBN: 9783540002925
Category : Medical
Languages : en
Pages : 238
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Book Description
Whilst genetic transformation of plants is commonly viewed as a means of bringing about plant improvement, it has not so readily been recognised as a tool for analysing the function of plant genes. This book is unusual in that it focuses on the genetic transformation of a range of plants using a number of different methods. Many plants have been found to be quite difficult to transform, and so various techniques were developed. These techniques include: Agrobacterium suspension drops, electroporation, PEG, "whiskers", and various biolistic methods. A chapter on intellectual and property rights is included.
Author: Charles Neal Stewart
Publisher: John Wiley & Sons
ISBN: 0470958944
Category : Science
Languages : en
Pages : 314
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Book Description
Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.
Author: Tzvi Tzfira
Publisher: Springer Science & Business Media
ISBN: 0387722904
Category : Science
Languages : en
Pages : 768
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Book Description
Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Author: Ingo Potrykus
Publisher: Springer Science & Business Media
ISBN: 3642792472
Category : Science
Languages : en
Pages : 370
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Book Description
Author: Adrian Slater
Publisher: OUP Oxford
ISBN: 0199282617
Category : Science
Languages : en
Pages : 401
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Book Description
Plant Biotechnology presents a balanced, objective exploration of the technology behind genetic manipulation, and its application to the growth and cultivation of plants. The book describes the techniques underpinning genetic manipulation and makes extensive use of case studies to illustrate how this influential tool is used in practice.
Author: Frank Kempken
Publisher: Springer Science & Business Media
ISBN: 3642023916
Category : Science
Languages : en
Pages : 683
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Book Description
Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book’s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.
Author: K. Lindsey
Publisher: Springer Science & Business Media
ISBN: 940090181X
Category : Science
Languages : en
Pages : 138
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Book Description
This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.
