Genetic Diversity of Proprietary Inbred Lines of Sunflower, Determined by Mapped SSR Markers and Total Protein Analysis PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Genetic Diversity of Proprietary Inbred Lines of Sunflower, Determined by Mapped SSR Markers and Total Protein Analysis PDF full book. Access full book title Genetic Diversity of Proprietary Inbred Lines of Sunflower, Determined by Mapped SSR Markers and Total Protein Analysis by Tertia Elizabeth Erasmus. Download full books in PDF and EPUB format.
Author: Tertia Elizabeth Erasmus
Publisher:
ISBN:
Category : Crops
Languages : en
Pages : 131
Get Book Here
Book Description
Author: Tertia Elizabeth Erasmus
Publisher:
ISBN:
Category : Crops
Languages : en
Pages : 131
Get Book Here
Book Description
Author: Jinguo Hu
Publisher: CRC Press
ISBN: 1439840490
Category : Science
Languages : en
Pages : 374
Get Book Here
Book Description
The sunflower has fascinated mankind for centuries. The oilseed sunflower contributes approximately ten percent of the world's plant-derived edible oil and the confection type sunflower holds a considerable share of the directly consumed snacks market. In addition, sunflower is also grown as an ornamental for cut flowers, as well as in home ga
Author: Melaku Ayele Gedil
Publisher:
ISBN:
Category : Sunflowers
Languages : en
Pages : 238
Get Book Here
Book Description
The level of polymorphisms of many biochemical and DNA markers are low in cultivated sunflower (Helianthus annuus L.). The number of mapped public DNA markers is limited. Molecular markers have not been developed for the most important diseases of sunflower, such as downy mildew. The objectives of this study were (i) to help alleviate the problem of low DNA marker polymorphisms by developing simple sequence repeat (SSR) markers, (ii) to build an integrated AFLP-RFLP linkage map by using previously described probes and newly developed AFLPs, and (iii) to clone and characterize candidate disease resistance genes. Forty-four polymorphic SSR markers were developed from a genomic DNA library. Diversity analysis of these SSRs for variability among 10 public inbred lines produced an average of 1.86 alleles per locus and mean heterozygosity of 0.21. The number of alleles ranged from 1 to 5. Trinucleotide SSRs were less polymorphic than dinucleotide and mononucleotide SSRs. Cluster analysis and multidimensional scaling separated elite inbred lines from wild species. There was more polymorphism in wild species than in elite lines. Three hundred and six AFLP markers were developed using 18 primer combinations. Two sets of previously mapped RFLP markers were tested for segregation in an F2 mapping population. A total of 401 markers were assigned to 17 linkage groups covering 1326 cM with a mean spacing of 3.3 cM between adjacent markers. The RFLP markers were well spaced and well distributed throughout the genome. Some linkage groups are sparsely populated with common markers. There were two gaps of 30 or more cM in two linkage groups. We cloned candidate disease resistance genes for downy mildew resistance based on sequence homology among resistance genes in other species. Eleven unique nucleotide binding sequence (NBS) containing clones were isolated and showed similarity to the corresponding domains of cloned disease resistance genes in other plant species. Seven clones mapped to four linkage groups and identified nine loci. A cleaved amplified polymorphic sequence (CAPS) marker that was 3.7 cM from the Pl1 resistance gene was developed by analysis of NILs. This CAPS marker should facilitate marker-assisted selection in sunflower.
Author: Carrie Freeman
Publisher:
ISBN:
Category : Genetic polymorphisms
Languages : en
Pages : 110
Get Book Here
Book Description
Single nucleotide polymorphisms (SNPs) are the most common DNA polymorphisms in plant and animal genomes. SNPs were identified in the allele sequences of up to 12 sunflower (Helianthus annuus L.) genotypes for a genome-wide sample of 81 loci originally mapped using restriction fragment length polymorphism (RFLP) markers. The RFLP loci anchor a dense RFLP linkage map and second-generation linkage maps constructed using simple sequence repeats and other high-throughput DNA markers. The goal of this study was to develop high-throughput SNP markers for the cDNA-RFLP loci for linkage mapping, diversity analysis, and molecular breeding and genomics research. The specific objectives were to: (i) develop FP-TDI or fluorescent primer-extension SNP assays for the cDNA-RFLP loci; (ii) validate the SNP markers by screening 12 sequenced (reference) genotypes; and (iii) assess the utility and polymorphism rate of the SNP markers across a genetically diverse panel of unsequenced wild and domesticated sunflower genotypes. SNP markers were successfully developed for 44 of the targeted 49 cDNA-RFLP loci. Thirty-one of the SNP markers were further selected for a diversity analysis across a genetically diverse panel of wild and domesticated sunflower genotypes. The mean heterozygosity (H) score for the loci was 0.41, which nears the maximum H score of 0.5 for biallelic markers. SNPs in this study exhibited polymorphism rates close to those of RFLP and SSR markers in inbred sunflower lines.
Author: Ju-Kyung Yu
Publisher:
ISBN:
Category : Sunflowers
Languages : en
Pages : 180
Get Book Here
Book Description
The cultivated sunflower (Helianthus annuus L., x=17) is one of the most important annual oilseed crops in the world. There are very few publicly shared sequence-based DNA markers and genetic maps in sunflower, even though molecular DNA markers and genetic maps have become widely used in all areas of genetic research and breeding in plant species. The objectives of this study were to develop sequence-based molecular markers and utilize the markers for genetic analyses and constructing maps in the cultivated sunflower. A total of 131 functional simple sequence repeat (SSR) markers were developed for 16 elite inbred lines using a small insert genomic library enriched for short simple sequence repeats. The polymorphism information content (PIC) estimated from 74 polymorphic SSR markers ranged from 0.0 to 0.93 with mean value of 0.55. Tetranucleotide repeats were significantly more polymorphic than dinucleotide and trinucleotide repeats, and no obvious correlation was found between repeat numbers and PIC scores. Genetic distance among 16 inbred lines, estimated from 74 polymorphic SSR markers ranged 0.175 to 0.543. Principal coordinate and cluster analyses of the genetic distance matrix well explained the difference between oilseed lines and confectionery lines, and sterility maintainer lines and fertility restorer lines. A total of 1,090 SSR markers were screened for polymorphism between the parents of two mapping populations. The two genetic maps were constructed by genotyping 94 recombinant inbred lines from a cross between PHA and PHB (276 SSR loci covering 1377.4 cM with mean distance of 4.99 cM), and 94 F2 progeny from a cross between HA370 and HA372 (122 SSR loci integrated into the existing RFLP framework map covering 1348.0 cM with mean distance of 6.77 cM). Ninety-three percent of the SSR markers were mapped to single loci and 56.5% of the loci were co-dominant. Clustering of SSR loci was observed near centromeric regions and most of the distorted loci were mapped to centromeric or distal regions. A concerted effort to develop SSR markers and generate highresolution SSR maps will enhance future fingerprinting analyses, fine-scale genome analyses and molecular breeding in the cultivated sunflower.
Author: K. Kamuraiah
Publisher:
ISBN:
Category :
Languages : en
Pages : 108
Get Book Here
Book Description
Author: Michael Lynn Kirsch
Publisher:
ISBN:
Category : Heterosis
Languages : en
Pages : 218
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Genetic diversity among 48 genotypes of confectionery sunflower (Helianthus annuus L.) collected from USDA, USA and India were evaluated by using microsatellite SSR markers. Twenty five sunflower specific SSR primers were used. Of the 25 SSR primers used, 10 primer pairs ( ORS331, ORS694, ORS728, ORS785, ORS807, ORS878, ORS 378, ORS1265,ORS1265,ORS1242) showed polymorphism. The high level of polymorphism (66.66%) was reported in this finding and the number of alleles in SSR loci ranged from 2 to 4 with an average of 2.5. The present study identifies the promising lines EC 734807, EC 734808,EC 734810,EC734860 and EC 734817 for protein and yield, these can be used as potential donors in future hybridization programme.
Author: K. V. Satyanarayana
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
Get Book Here
Book Description
Author: Suzanne Christine Savin
Publisher:
ISBN:
Category : Genetic polymorphisms
Languages : en
Pages : 112
Get Book Here
Book Description
Helianthus schweinitzii T. & G. (Asteraceae) is a federally endangered sunflower endemic to the Piedmont region of North and South Carolina. Anthropogenic habitat alteration has reduced this species into isolated population groups. Previous genetic surveys revealed these populations were genetically similar and the species, as a whole, exhibited moderate levels of genetic variation. We used a genetic marker more sensitive to genetic polymorphisms to corroborate these findings. Analysis of four polymorphic microsatellite loci also indicated that populations of Schweinitz's sunflower are genetically similar and genetic distances are not correlated with geographic distances. Moderate levels of variability and heterozygosities higher than those of other rare endemic species were observed. We conclude that mitigation programs that translocate populations from geographically distant areas into a common preserve remains an acceptable conservation strategy that is unlikely to result in outbreeding depression.
