Genetic and Genomic Approaches to Dissect Nodulation in the Model Legume Medicago Truncatula PDF Download
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Author: Kavitha Tharmia Kuppusamy
Publisher:
ISBN:
Category :
Languages : en
Pages : 278
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Author: Kavitha Tharmia Kuppusamy
Publisher:
ISBN:
Category :
Languages : en
Pages : 278
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Author: Nandini Krishnamurthy
Publisher:
ISBN:
Category :
Languages : en
Pages : 198
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Author: Senjuti Sinharoy
Publisher: Springer Nature
ISBN: 3030907570
Category : Science
Languages : en
Pages : 177
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Book Description
This book focuses on the discoveries in M. truncatula genomic research which has been undertaken in the last two decades. Legumes are important for their economic values as food, feed, and fodder and also serve as the pillar of sustainable agriculture because of its biological nitrogen fixation capacity. Medicago truncatula was established as a model legume in the 1990s and has been well adopted as a model internationally since then. M. truncatula is an autogamous, diploid (2n = 16) species with a short generation time, and relatively small genome size (~375 Mbp). The M. truncatula genome was initially sequenced by the International Medicago Genome Annotation Group (IMGAG) in 2011 and has been well-annotated. M. truncatula research benefits from the availability of several genetic and genomic tools, such as gene expression atlas (MtGEA), insertion and neutron bombardment mutant populations, and a HapMap panel containing 384 sequenced inbred lines for genome-wide association studies. This book covers the current status and latest advancements of the M. truncatula genomics and transcriptomics resources along with a glimpse of newly developed tools that makes M. truncatula a front runner model in functional genomic studies.
Author: Luis A. Cañas
Publisher:
ISBN: 9781493986330
Category :
Languages : en
Pages : 343
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Book Description
Author: K. Nicole Holland
Publisher:
ISBN:
Category :
Languages : en
Pages : 80
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Book Description
Nitrogen is an essential nutrient required by all plants. Although the earth's atmosphere consists of approximately 78% nitrogen, it exists in an unusable form for plants. Current agricultural practices rely on industrial nitrogen fixation to produce fertilizers that improve crop productivity. However, this method requires the use of precious natural resources and can cause a variety of negative impacts on the environment and human health. Biologically fixed nitrogen is a favorable alternative accessible to leguminous plants through their symbiotic relationship with soil bacteria called rhizobia. Genetic studies aimed at identifying plant genes related to successful nodulation and nitrogen fixation are underway to help better understand this infection process and improve the performance of legumes in agriculture. To date, over 21,000 Tnt1 mutant lines of Medicago truncatula, a model legume used for genetic research, have been created. Several lines displaying defects in symbiotic nitrogen fixation (SNF) have been identified and are referred to as Fix- mutants. In this research, segregation analysis of the Fix- mutant NF18598 indicated loss of SNF function could be governed by a dominant mutation. A total of 114 Tnt1 insertions were identified in the NF18598 genome using three different sequencing methods. Tnt1-capture sequencing (SC) found the largest number of insertions (70) while the other two methods, thermal asymmetric interlaced PCR (TAIL-PCR) and whole genome sequencing (WGS), found a similar number of insertions (40 and 41, respectively). It was found that 50% of the insertions were located in coding regions. One of the Tnt1 insertions located on chromosome 5 is found within the exon of a phosphatase 2C family protein gene (Medtr5g009370) and is upregulated in nodules. While there was a significant SNF association with Medtr5g009370 in the R1 generation, this association was not detected in the R2 generation. Light microscopy analysis of nodules displayed clear differences between mutant samples identified as either Fix+ or Fix-. The Fix- mutant nodules displayed some cellular organization but distinct developmental zones could not be identified. Infection threads were observed in Fix- nodules that appeared to contain bacteria that had not been released; however, further microscopy analysis must be conducted to verify the presence of rhizobia. In addition, further research involving a larger sample population would help verify if the insertion located in Medtr5g009370 is responsible for the defective nodule phenotype observed and if the phosphatase gene is involved in symbiotic nitrogen fixation.
Author: Rajeev K. Varshney
Publisher: Springer
ISBN: 3319661175
Category : Science
Languages : en
Pages : 152
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Book Description
This book sheds new light on the chickpea genome sequencing and resequencing of chickpea germplasm lines and provides insights into classical genetics, cytogenetics, and trait mapping. It also offers an overview of the latest advances in genome sequencing and analysis. The growing human population, rapid climate changes and limited amounts of arable land are creating substantial challenges in connection with the availability and affordability of nutritious food for smallholder farmers in developing countries. In this context, climate smart crops are essential to alleviating the hunger of the millions of poor and undernourished people living in developing countries. In addition to cereals, grain legumes are an integral part of the human diet and provide sustainable income for smallholder farmers in the arid and semi-arid regions of the world. Among grain legumes, the chickpea (Cicer arietinum) is the second most important in terms of production and productivity. Besides being a rich source of proteins, it can fix atmospheric nitrogen through symbiosis with rhizobia and increase the input of combined nitrogen. Several abiotic stresses like drought, heat, salinity, together with biotic stresses like Fusarium wilt, Ascochyta blight, and Botrytis grey mould have led to production losses, as the chickpeas is typically grown in the harsh climates of our planet’s semi-arid regions.
Author: K.A. Lesins
Publisher: Springer Science & Business Media
ISBN: 9400996349
Category : Science
Languages : en
Pages : 235
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Book Description
In introducing ourselves it should be told that in our native Latvian language our name is written LesiJ;l. s. In most English publications, as in this work, the writing has been simplified to Lesins, and often only the first initial has been used. Our interest in Medicago was first aroused during 1936-38, while em ployed as teachers in the agricultural and home economics school at Be brene, Upper Zemgale, Latvia. Some plants of alfalfa (M. varia, M. media), locally called 'lucema', were found growing wild along roadsides in that area, though no alfalfa fields had been seen in the vicinity within the me mory of local farmers. Some roadside plants were dug out and transplanted to the garden, but their seedset was poor. During the next few years we paid only slight attention to alfalfa, the reason being that Latvia is a country with Atlantic climatic features (annual precipitation 600-700 mm; mild win ters for its 56°-58° N. Lat. , with January isotherms between -3° and -7° C; moderately warm summers, with July isotherms between 16° and 18° C), which together with its soils, mostly of acidic, podzolic type, is not well suited for alfalfa production. It was not until 1945 in Sweden that work on alfalfa came to the foreground, when the senbor author was assigned investi gations on alfalfa seed setting by Dr. Erik Akerberg, then director of the Swedish Seed Association branch station at ffituna.
Author: Claudine Elmerich
Publisher: Springer Science & Business Media
ISBN: 9780792348344
Category : Science
Languages : en
Pages : 732
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Book Description
Nitrogen availability is one of the most critical factors that limits plant productivity. The largest reservoir of nitrogen is the atmosphere, but this gaseous molecular nitrogen only becomes available to plants through the biological nitrogen fixation process, which only prokaryotic cells have developed. The discovery that microbes were providing fixed nitrogen to legumes and the isolation of the first nitrogen-fixing bacteria occured at the end the 19th Century, in Louis Pasteur's time. We are now building on more than 100 years of research in this field and looking towards the 21st Century. The International Nitrogen Fixation Congress series Started more than 20 years ago. The format of this Congress is designed to gather scientists from very diverse origins, backgrounds, interests and scientific approaches and is a forum where fundamental knowledge is discussed alongside applied research. This confluence of perspectives is, we believe, extremely beneficial in raising new ideas, questions and concepts.
Author: Ray Rose
Publisher: Humana Press
ISBN: 9781627036122
Category : Science
Languages : en
Pages : 0
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Book Description
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
Author: S. M. Paul Khurana
Publisher: Springer Nature
ISBN: 9811384991
Category : Science
Languages : en
Pages : 670
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Book Description
Refinement in sequencing technologies and potential of genomic research resulted in meteoric growth of biological information such as sequences of DNA, RNA and protein requiring databases for efficient storage, management and retrieval of the biological information. Also, computational algorithms for analysis of these colossal data became a vital aspect of biological sciences. The work aims to show the process of turning bioscience innovation into companies and products, covering the basic science, the translation of science into technology. Due to rapid developments, there seems to be no basic difference between the pharmaceutical industry and the biotechnological industry. However, approved products in the pipeline and renewed public confidence make it one of the most promising areas of economic growth in the near future. India offers a huge market for the products as well as cheap manufacturing base for export. The book is a sincere work of compilation of new and recent advances in the topic of concern through various innovative researches and scientific opinion therefrom. The book is dedicated to the readers who will definitely find it interesting and knowledgeable in carrying out their respective researches in different aspects of applied microbiology and biotechnology.
