Genetic and Functional Analysis of Synaptic CA2+ Dynamics in Drosophila PDF Download
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Author: Xiaomin Xing
Publisher:
ISBN:
Category : Calcium
Languages : en
Pages : 132
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Book Description
Ca2+ influx is one of the critical events that trigger synaptic vesicular release, and the accumulation of residual free Ca2+ in synapses is also important for activity-dependent synaptic plasticity. Ca2+ imaging with fluorescence indicators (synthetic or genetically encoded) is a powerful approach to monitor Ca2+ levels in neurons and synapses. Although accumulating studies in vertebrate systems have been carried out to demonstrate the role of Ca2+ in synaptic transmission and plasticity, most of these studies rely on pharmacological methods to infer the molecular mechanism, with less emphasis on forward genetic analysis. The Drosophila neuromuscular junction (NMJ) is a powerful neurogenetic platform for studying synaptic transmission, because of the availability of many mutations. However, not many mutations have been analyzed with Ca2+ imaging. Besides, although Genetically Encoded Ca2+ Indicators (GECIs) including GCaMPs are increasingly popular as the tool to identify neuronal circuits activated by certain stimuli or mediating particular behaviors, the physiological and functional interpretation of neuronal Ca2+ transients reported by GECIs remain obscure. By expressing GCaMPs in NMJ synapses, I characterized a spectrum of genetic mutations including sodium channel alleles parats1, parabss1, potassium channel mutations Shaker (ShM, Sh120), Shab3, ether-a-go-go (eag1, eag4pm), and double mutant eag1 Sh120. Drosophila NMJs contain at least three different types of synapses, which include glutamatergic tonic motor synapse type Ib, phasic motor synapse type Is, and modulatory octopaminergic synapse type II. In this study, I found that the ion channel mutations did not uniformly alter the Ca2+ dynamics in type Ib, Is and II synapses.
Author: Xiaomin Xing
Publisher:
ISBN:
Category : Calcium
Languages : en
Pages : 132
Get Book Here
Book Description
Ca2+ influx is one of the critical events that trigger synaptic vesicular release, and the accumulation of residual free Ca2+ in synapses is also important for activity-dependent synaptic plasticity. Ca2+ imaging with fluorescence indicators (synthetic or genetically encoded) is a powerful approach to monitor Ca2+ levels in neurons and synapses. Although accumulating studies in vertebrate systems have been carried out to demonstrate the role of Ca2+ in synaptic transmission and plasticity, most of these studies rely on pharmacological methods to infer the molecular mechanism, with less emphasis on forward genetic analysis. The Drosophila neuromuscular junction (NMJ) is a powerful neurogenetic platform for studying synaptic transmission, because of the availability of many mutations. However, not many mutations have been analyzed with Ca2+ imaging. Besides, although Genetically Encoded Ca2+ Indicators (GECIs) including GCaMPs are increasingly popular as the tool to identify neuronal circuits activated by certain stimuli or mediating particular behaviors, the physiological and functional interpretation of neuronal Ca2+ transients reported by GECIs remain obscure. By expressing GCaMPs in NMJ synapses, I characterized a spectrum of genetic mutations including sodium channel alleles parats1, parabss1, potassium channel mutations Shaker (ShM, Sh120), Shab3, ether-a-go-go (eag1, eag4pm), and double mutant eag1 Sh120. Drosophila NMJs contain at least three different types of synapses, which include glutamatergic tonic motor synapse type Ib, phasic motor synapse type Is, and modulatory octopaminergic synapse type II. In this study, I found that the ion channel mutations did not uniformly alter the Ca2+ dynamics in type Ib, Is and II synapses.
Author:
Publisher: Academic Press
ISBN: 0080857779
Category : Science
Languages : en
Pages : 317
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Book Description
Neuromuscular Junctions in Drosophila gathers the main contributions that research using the fruit fly Drosophila melanogaster has made in the area of synapse development, synapse physiology, and excitability of muscles and nerve cells. The chapters in this book represent a synthesis of major advances in our understanding of neuronal development and synaptic physiology, which have been obtained using the above approach.This book is directed to the general neuroscience audience: researchers, instructors, graduate students, and advanced undergraduates who are interested in the mechanisms of synapse development and physiology. However, the book will also be a valuable resource for those that use the fruit fly as a model system in their laboratories. Key Features* Synthesizes the genetic approaches used to study synaptic development and function at the neuromuscular junction, using flies as a model system* Covers major recent advances in muscle development, pathfinding, synapse maturation and plasticity, exo- and endocytosis, and ion channel function* Written in clear language that is easily understandable to readers not already familiar with fruit fly research* Includes numerous diagrams and extensive reference lists
Author: Hugo J. Bellen
Publisher: Oxford University Press, USA
ISBN:
Category : Medical
Languages : en
Pages : 466
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Book Description
This book provides the reader with background information on neurotransmitter release. Emphasis is placed on the rationale by which proteins are assigned specific functions rather than just providing facts about function.
Author: Rodolfo Riascos Llinás
Publisher: Oxford University Press, USA
ISBN: 9780195116526
Category : Neural transmission
Languages : en
Pages : 234
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Book Description
Ideal for graduate and undergraduate courses, the book includes PC and Macintosh versions of two programs for simulating and manipulating any aspect of synaptic transmission."--BOOK JACKET.
Author: Federico Bermudez-Rattoni
Publisher: CRC Press
ISBN: 1420008412
Category : Psychology
Languages : en
Pages : 368
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Book Description
A comprehensive, multidisciplinary review, Neural Plasticity and Memory: From Genes to Brain Imaging provides an in-depth, up-to-date analysis of the study of the neurobiology of memory. Leading specialists share their scientific experience in the field, covering a wide range of topics where molecular, genetic, behavioral, and brain imaging techniq
Author: Josh Dubnau
Publisher: Cambridge University Press
ISBN: 1107009030
Category : Medical
Languages : en
Pages : 309
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Book Description
A comprehensive portrayal of the behaviour genetics of the fruit fly (Drosophila melanogaster) and the methods used in these studies.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author:
Publisher:
ISBN:
Category : Neurology
Languages : en
Pages : 442
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Book Description
Author:
Publisher: Academic Press
ISBN: 0080951155
Category : Science
Languages : en
Pages : 218
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Book Description
Genes interact with the environment, experience, and biology of the brain to shape an animal’s behavior. This latest volume in Advances in Genetics, organized according to the most widely used model organisms, describes the latest genetic discoveries in relation to neural circuit development and activity. Explores the latest topics in neural circuits and behavior research in zebrafish, drosophila, C.elegans, and mouse models Includes methods for testing with ethical, legal, and social implications Critically analyzes future prospects
Author: William Sullivan
Publisher: CSHL Press
ISBN: 9780879695866
Category : Medical
Languages : en
Pages : 798
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Book Description
This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.
