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Author: Mathieu Vinken
Publisher:
ISBN: 9781493936649
Category : Gap junctions (Cell biology)
Languages : en
Pages : 235
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Book Description
Author: Mathieu Vinken
Publisher:
ISBN: 9781493936649
Category : Gap junctions (Cell biology)
Languages : en
Pages : 235
Get Book Here
Book Description
Author: Taylor & Francis Group
Publisher: CRC Press
ISBN: 9780367658441
Category :
Languages : en
Pages : 289
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Book Description
Gap junction channels are a group of intercellular channels expressed in tissues and organs to synchronize many physiological processes. A gap junction channel is formed by the docking of two hemichannels, and each hemichannel is a hexamer of connexins. The field of gap junction channel and hemichannel research has recently exploded and became one of the most active areas of cell biology. Numerous novel approaches and techniques have been developed, but there is no single book dedicated to the unique techniques and protocols employed for the research on these large pore channels. This book fills the gap and focuses on protocols, approaches and reviews of gap junction channels and connexin hemichannels. It will be a useful reference for graduate students, postdoctoral fellows and researchers. Anyone with an interest in gap junction channels and hemichannels will need this summary of state-of-the-art techniques and protocols.
Author: Roberto Bruzzone
Publisher: Springer Science & Business Media
ISBN: 1592590438
Category : Science
Languages : en
Pages : 495
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Book Description
Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.
Author: Rudolf Werner
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 412
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Book Description
Author: Donglin Bai
Publisher:
ISBN: 9781315369396
Category : Connexins
Languages : en
Pages : 289
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Book Description
Author: Michael Vander Laan Bennett
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 440
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Book Description
Author: Elliot L. Hertzberg
Publisher: Alan R. Liss
ISBN:
Category : Science
Languages : en
Pages : 578
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Book Description
Author: Jianghui Hou
Publisher: Academic Press
ISBN: 0128226439
Category : Science
Languages : en
Pages : 422
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Book Description
A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels
Author: Lilianna Solnica-Krezel
Publisher: Springer
ISBN: 9783642536670
Category : Science
Languages : en
Pages : 438
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Book Description
Authored by leading experts in the field, this book provides the first comprehensive overview of the mechanisms of early patterning and morphogenesis in zebrafish. It summarizes the current knowledge and the key questions for the next decade of research.
Author: M.D. Peracchia
Publisher: CRC Press
ISBN: 1351087193
Category : Medical
Languages : en
Pages : 606
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Book Description
This book provides a state of the art account of present knowledge of the biophysics of cell-to-cell channels. It is divided into two sections, one dealing with two-cell systems and the other with reconstitution systems.
