Fundamentals of Light Microscopy and Electronic Imaging PDF Download
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Author: Douglas B. Murphy
Publisher: John Wiley & Sons
ISBN: 047169214X
Category : Medical
Languages : en
Pages : 564
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Book Description
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Author: Douglas B. Murphy
Publisher: John Wiley & Sons
ISBN: 047169214X
Category : Medical
Languages : en
Pages : 564
Get Book Here
Book Description
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Author: Douglas B. Murphy
Publisher:
ISBN:
Category :
Languages : en
Pages : 538
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Book Description
Author: Shinya Inoue
Publisher: Springer Science & Business Media
ISBN: 1475769253
Category : Medical
Languages : en
Pages : 600
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Book Description
Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.
Author: Jerome Mertz
Publisher: Cambridge University Press
ISBN: 1108428304
Category : Medical
Languages : en
Pages : 475
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Book Description
Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.
Author: Partha Pratim Mondal
Publisher: Springer Science & Business Media
ISBN: 9400775458
Category : Science
Languages : en
Pages : 219
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Book Description
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Author: Yang Leng
Publisher: John Wiley & Sons
ISBN: 0470822996
Category : Technology & Engineering
Languages : en
Pages : 384
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Book Description
This book covers state-of-the-art techniques commonly used in modern materials characterization. Two important aspects of characterization, materials structures and chemical analysis, are included. Widely used techniques, such as metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are described. In addition, the book introduces advanced techniques, including scanning probe microscopy. The second half of the book accordingly presents techniques such as X-ray energy dispersive spectroscopy (commonly equipped in the scanning electron microscope), fluorescence X-ray spectroscopy, and popular surface analysis techniques (XPS and SIMS). Finally, vibrational spectroscopy (FTIR and Raman) and thermal analysis are also covered.
Author: Gabriel Cristobal
Publisher: John Wiley & Sons
ISBN: 3527635254
Category : Technology & Engineering
Languages : en
Pages : 949
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Book Description
In recent years, Moore's law has fostered the steady growth of the field of digital image processing, though the computational complexity remains a problem for most of the digital image processing applications. In parallel, the research domain of optical image processing has matured, potentially bypassing the problems digital approaches were suffering and bringing new applications. The advancement of technology calls for applications and knowledge at the intersection of both areas but there is a clear knowledge gap between the digital signal processing and the optical processing communities. This book covers the fundamental basis of the optical and image processing techniques by integrating contributions from both optical and digital research communities to solve current application bottlenecks, and give rise to new applications and solutions. Besides focusing on joint research, it also aims at disseminating the knowledge existing in both domains. Applications covered include image restoration, medical imaging, surveillance, holography, etc... "a very good book that deserves to be on the bookshelf of a serious student or scientist working in these areas." Source: Optics and Photonics News
Author: Suzanne Bell
Publisher: CRC Press
ISBN: 1439882436
Category : Law
Languages : en
Pages : 182
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Book Description
Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines. Designed to serve as a primary reso
Author: Douglas E. Chandler
Publisher: Jones & Bartlett Publishers
ISBN: 0763738743
Category : Medical
Languages : en
Pages : 468
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Book Description
The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Author: Alexei A. Maradudin
Publisher: Cambridge University Press
ISBN: 1139497871
Category : Science
Languages : en
Pages : 459
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Book Description
Optical metamaterials are an exciting new field in optical science. A rapidly developing class of these metamaterials are those that allow the manipulation of volume and surface electromagnetic waves in desirable ways by suitably structuring the surfaces they interact with. They have applications in a variety of fields, such as materials science, photovoltaic technology, imaging and lensing, beam shaping and lasing. Describing techniques and applications, this book is ideal for researchers and professionals working in metamaterials and plasmonics, as well as those just entering this exciting new field. It surveys different types of structured surfaces, their design and fabrication, their unusual optical properties, recent experimental observations and their applications. Each chapter is written by an expert in that area, giving the reader an up-to-date overview of the subject. Both the experimental and theoretical aspects of each topic are presented.
