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Author: Yingfu Li
Publisher: Springer Science & Business Media
ISBN: 0387737111
Category : Science
Languages : en
Pages : 396
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Book Description
Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.
Author: Yingfu Li
Publisher: Springer Science & Business Media
ISBN: 0387737111
Category : Science
Languages : en
Pages : 396
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Book Description
Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.
Author: Kazuhiko Nakatani
Publisher: Springer
ISBN: 3319271113
Category : Science
Languages : en
Pages : 280
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Book Description
This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.
Author: Yunbo Luo
Publisher: Springer
ISBN: 9811082197
Category : Science
Languages : en
Pages : 360
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Book Description
This book highlights the development of a functional nucleic acid based biosensor detection method in the context of food safety. Although there have been major advances in food processing technology in both developed and developing countries, food safety assurance systems are generally becoming more stringent, in response to growing (both real and perceived) food safety problems. These problems are due in part to foodborne microorganisms, heavy metals, and small chemical molecules (biological toxins, pesticide residues, and veterinary drug residues), etc. In addition, the nucleic acid biomarkers (DNA methylation, microRNA, and circRNA) induced by these risk factors are also closely related to food safety. Accordingly, this book offers a brief guide to targets and strategies in functional nucleic acid based biosensors for food safety detection. Divided into several chapters that focus on various respective targets, it will be a valuable resource for students and researchers in the fields of biosensor detection, food science etc.
Author: Sven Klussmann
Publisher: John Wiley & Sons
ISBN: 3527607919
Category : Science
Languages : en
Pages : 518
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Book Description
In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.
Author: G Michael Blackburn
Publisher: Royal Society of Chemistry
ISBN: 1782625771
Category : Science
Languages : en
Pages : 503
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Book Description
The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.
Author: Marco Mascini
Publisher: Royal Society of Chemistry
ISBN: 1849732698
Category : Science
Languages : en
Pages : 187
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Book Description
Nucleic acids are the fundamental building blocks of life and are found in all living things. In recent years, their functions have been shown to extend beyond the Watson-Crick base pair recognition of complementary strands. Molecules (known as aptamers) consisting of 40-50 nucleotides have been isolated that are able to bind a broad range of molecules with high affinity and specificity. The molecules recognized by aptamers range from small organic molecules to proteins, cells and even intact viral particles. Catalytic DNA molecules called NAzymes (RNAzyme or DNAzyme) have also been shown to exist and, when combined with aptamers, are known as aptazymes. These biomolecules can be used to develop smart and innovative biosensors for environmental analysis. Monitoring of contaminants in the air, water and soil is a key component in understanding and managing risks to human health and ecosystems. This, in conjunction with the time and cost involved in traditional chemical analysis, means there is a growing need for simple, rapid, cost-effective and portable screening methods. Biosensors are compact devices which complement current field screening and monitoring methods. This book demonstrates the incredible opportunities that nucleic acids can offer to environmental analytical chemistry. The chapters: show how nucleic acids have a pivotal role in the development of smart biosensors for environmental monitoring; describe the development of biosensors based on aptamers and NAzymes for the detection of organic and inorganic pollutants; deal with the use of nucleic acid based biosensors for environmental toxicity screening, and detail the use of nanomaterials, as well as miniaturization and lab-on-a-chip technologies, for nucleic acid based biosensing systems.
Author: Richard Durbin
Publisher: Cambridge University Press
ISBN: 113945739X
Category : Science
Languages : en
Pages : 372
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Book Description
Probabilistic models are becoming increasingly important in analysing the huge amount of data being produced by large-scale DNA-sequencing efforts such as the Human Genome Project. For example, hidden Markov models are used for analysing biological sequences, linguistic-grammar-based probabilistic models for identifying RNA secondary structure, and probabilistic evolutionary models for inferring phylogenies of sequences from different organisms. This book gives a unified, up-to-date and self-contained account, with a Bayesian slant, of such methods, and more generally to probabilistic methods of sequence analysis. Written by an interdisciplinary team of authors, it aims to be accessible to molecular biologists, computer scientists, and mathematicians with no formal knowledge of the other fields, and at the same time present the state-of-the-art in this new and highly important field.
Author: Orkun S. Soyer
Publisher: Springer Science & Business Media
ISBN: 1461435676
Category : Science
Languages : en
Pages : 459
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Book Description
The book aims to introduce the reader to the emerging field of Evolutionary Systems Biology, which approaches classical systems biology questions within an evolutionary framework. An evolutionary approach might allow understanding the significance of observed diversity, uncover “evolutionary design principles” and extend predictions made in model organisms to others. In addition, evolutionary systems biology can generate new insights into the adaptive landscape by combining molecular systems biology models and evolutionary simulations. This insight can enable the development of more detailed mechanistic evolutionary hypotheses.
Author: Chad A. Mirkin
Publisher: CRC Press
ISBN: 0429578067
Category : Medical
Languages : en
Pages : 1866
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Book Description
Spherical nucleic acids (SNAs) comprise a nanoparticle core and a densely packed and highly oriented nucleic acid shell, typically DNA or RNA. They have novel architecture-dependent properties that distinguish them from all other forms of nucleic acids and make them useful in materials synthesis, catalysis, diagnostics, therapeutics, and optics/plasmonics. This book covers over two decades of Dr. Mirkin’s research on SNAs and their anisotropic analogues, including synthesis and fundamental properties, and applications in colloidal crystallization, adaptive matter, and nanomedicine, spanning extra- and intracellular diagnostics, gene regulation, and immunomodulation. It is a reprint volume that compiles 101 key papers from high-impact journals in this research area published by the Mirkin Group at Northwestern University, Illinois, USA, within the International Institute for Nanotechnology, and collaborators. Volume 1 provides an overview and a historical framework of engineering matter from DNA-modified constructs and discusses the enabling features of nucleic acid–functionalized nanomaterials. Volume 2 covers design rules for colloidal crystallization, building blocks for crystal engineering, and DNA and RNA as programmable bonds. Volume 3 discusses colloidal crystallization processes and routes to hierarchical assembly, dynamic nanoparticle superlattices, surface-based and template-confined colloidal crystallization, optics and plasmonics with nanoparticle superlattices, and postsynthetic modification and catalysis with nanoparticle superlattices. Volume 4 covers diagnostic modalities, and intracellular therapeutic and diagnostic schemes based upon nucleic acid–functionalized nanomaterials.
Author: Peter E. Nielsen
Publisher: Springer Science & Business Media
ISBN: 1592592902
Category : Science
Languages : en
Pages : 270
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Book Description
Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .
