Fluorescence In-Situ Hybridization (FISH) for Microbial Cells PDF Download
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Author: Nuno F. Azevedo
Publisher: Humana
ISBN: 9781071611142
Category : Science
Languages : en
Pages : 335
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Book Description
This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.
Author: Nuno F. Azevedo
Publisher: Humana
ISBN: 9781071611142
Category : Science
Languages : en
Pages : 335
Get Book Here
Book Description
This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.
Author: Thomas Liehr
Publisher: Springer Science & Business Media
ISBN: 3540705813
Category : Science
Languages : en
Pages : 447
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Book Description
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Author: Gal Haimovich
Publisher: Springer Nature
ISBN: 1071637665
Category :
Languages : en
Pages : 303
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Book Description
Author: Thomas Liehr
Publisher: Springer
ISBN: 9783540705802
Category : Science
Languages : en
Pages : 451
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Book Description
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Author: Joanna M. Bridger
Publisher: Humana
ISBN: 9781493957248
Category : Science
Languages : en
Pages : 451
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Book Description
Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.
Author: Jan David Brüwer
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Microorganisms dominate the surface ocean and significantly shape biogeochemical cycles, particularly the carbon cycle. Through photosynthesis, primary producers fix inorganic carbon and produce organic matter. The phytoplankton-derived organic matter fuels differently specialized heterotrophic bacteria that remineralize large proportions of it. At the same time, phages, which are viruses that infect bacteria, shape the bacterial community composition through top-down controls. Environmental microbial ecology often uses microscopically derived changes in cell abundance or incubation experiments with labeled substrates to derive bacterial growth or activity. However, the former does not account for mortality, while the latter is prone to biases by bottle effects and substrate preferences. Nevertheless, marine microbial ecology requires a thorough understanding of bacterial growth and mortality to understand the effects on, e.g., the carbon cycle. Additionally, microbial ecologists have little understanding of the effect of phages on the heterotrophic community. For example, no studies have quantified the amount of phage-infected heterotrophs in complex marine samples to date. For this thesis, I studied the bacterial life cycle, from cell division to cell death, in environmental samples. To do so, I used fluorescence in situ hybridization (FISH) techniques and high-throughput image cytometry, complemented with metagenomic analyses. A significant focus was on bacteria of the SAR11 clade. SAR11 are specialized to grow in oligotrophic habitats that are nutrient and substrate-depleted. They are assumed to be outcompeted by specialized bacteria during high-substrate conditions, such as phytoplankton blooms. Additionally, there is an ongoing debate on whether phage infection has a considerable effect on SAR11 communities. In this thesis, I demonstrate rapidly dividing SAR11 communities during phytoplankton blooms with a concomitantly high phage infection rate. In Chapter 2, I used FISH in combination with a fluorescent DNA stain to study the frequency of dividing cells by visualizing the intracellular DNA distribution. During a cell replication cycle, the dublicated genomes need to be separated into the future daughter cells. The correlation with experimentally derived in situ cell division rates allowed the calculation of cell division across an entire phytoplankton bloom. Measurements revealed faster SAR11 cell division rates than anticipated by cell counts. Hence, calculated mortality rates were high during these times. As the mortality was taxon-specific, I hypothesized phage-induced lysis as a cause. In Chapter 3, I designed direct-geneFISH probes to visualize phage-infected SAR11 cells in the environment. I could thereby provide the missing link between cell division and mortality rates, as revealed in Chapter 2. The highest amounts of phage-infected SAR11 cells (up to 19 % of the SAR11 cells) were detected when taxon-specific mortality and cell division rates were highest. Additionally, I found a phenomenon of phage-infected, ribosome-depleted cells, which I dubbed 'zombie cells'. I thoroughly discuss possible explanations for their emergence and propose that nucleotides from ribosomal RNA are used as substrates for phage genome synthesis. Additionally, I show that both phage-infected and zombie cells occur globally. In Chapter 4, I assessed the influences of future ocean scenarios and a marine heatwave on the microbial community, including cell division and grazing rates. Anthropogenic influences shape the ocean as a habitat with unknown consequences for the microbial community. I found no significant influences of the mild marine heatwave, while the future ocean scenarios caused differences in bacterial abundances. Overall, the results indicate a stable and adaptable marine microbial community in the face of a changing ocean. In the general discussion (Chapter 5), I discuss the methodological approaches used in this thesis to study bacterial cell division rate and the viral community. I further summarize and discuss the insights into SAR11 ecology gained through this thesis, as well as the importance and emergence of zombie cells. The discussion is rounded off with an outlook, proposing directions for future research projects to understand further the interplay of bacterial hosts and their phages, as well as zombie cells.
Author: Bernd W. Rautenstrauß
Publisher: Springer Science & Business Media
ISBN: 3642564046
Category : Science
Languages : en
Pages : 493
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Book Description
Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Author: M. H. F. Wilkinson
Publisher: John Wiley & Sons
ISBN: 9780471974406
Category : Science
Languages : en
Pages : 588
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Book Description
Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.
Author:
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages :
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Book Description
Author: Michael Andreeff
Publisher: Wiley-Liss
ISBN: 9780471013457
Category : Medical
Languages : en
Pages : 0
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Book Description
Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. The final section of the book is devoted to the applications of FISH techniques to genetic analysis. This incorporates radiation biodosimetry in chromosomal aberrations, genetic toxicology, and multiprobe methods for detecting chromosomally defective sperm in humans and mice, plus rapid prenatal diagnosis of chromosomal aneuploidies and detection of fetal trisomy. Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry.
