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Author: Marion G. Macey
Publisher: Springer Science & Business Media
ISBN: 1597454516
Category : Medical
Languages : en
Pages : 289
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Book Description
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Author: Marion G. Macey
Publisher: Springer Science & Business Media
ISBN: 1597454516
Category : Medical
Languages : en
Pages : 289
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Book Description
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Author:
Publisher: Elsevier
ISBN: 0080859399
Category : Science
Languages : en
Pages : 633
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Book Description
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.Key Features* Completely revised and greatly expanded since the publication of the First Edition in 1990* Methods cover cell death and cell cycle analysesPractical, handbook-style presentation works in lab or classroom* Unique comprehensive methodological coverage* Color plates illustrate techniques* In-depth treatment of procedures, including a description of each procedure:* Theoretical foundations* Critical aspects* Possible pitfalls* Written by authors with extensive experience who:* Developed or modified the techniques* Describe their experience with different instruments and applications to different cell systems* Are the Who's Who in Flow Cytometry
Author: Howard M. Shapiro
Publisher: John Wiley & Sons
ISBN: 0471434035
Category : Science
Languages : en
Pages : 736
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Book Description
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Author: Christine Goetz
Publisher: Springer
ISBN: 3319980718
Category : Medical
Languages : en
Pages : 229
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Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Author: Alice Longobardi Givan
Publisher: John Wiley & Sons
ISBN: 1118688392
Category : Science
Languages : en
Pages : 309
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Book Description
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Author: Zbigniew Darzynkiewicz
Publisher: Elsevier
ISBN: 9780125441674
Category : Cytogenetics
Languages : en
Pages : 670
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Book Description
Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.
Author: Teresa S. Hawley
Publisher: Springer Nature
ISBN: 107163738X
Category :
Languages : en
Pages : 460
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Book Description
Author: Awtar Krishan
Publisher: John Wiley & Sons
ISBN: 1118148061
Category : Science
Languages : en
Pages : 317
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Book Description
A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
Author: Jaroslav Dolezel
Publisher: John Wiley & Sons
ISBN: 3527610936
Category : Science
Languages : en
Pages : 479
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Book Description
Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.
Author: M. G. Ormerod
Publisher: Damaris Publishing
ISBN: 9780955981203
Category : Flow cytometry
Languages : en
Pages : 116
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Book Description
Flow cytometry is a technique used to study cells, such as blood cells or cancer cells. It is used in medical and research laboratories.
