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Author: Davide Vigetti
Publisher: Humana Press
ISBN: 9781493991327
Category : Science
Languages : en
Pages : 278
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Book Description
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.
Author: Davide Vigetti
Publisher: Humana Press
ISBN: 9781493991327
Category : Science
Languages : en
Pages : 278
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Book Description
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.
Author: Francesco Travascio
Publisher: BoD – Books on Demand
ISBN: 9535124153
Category : Science
Languages : en
Pages : 376
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Book Description
The extracellular matrix (ECM) is an ensemble of non-cellular components present within all tissues and organs of the human body. The ECM provides structural support for scaffolding cellular constituents and biochemical and biomechanical support for those events leading to tissue morphogenesis, differentiation and homeostasis. Essential components of all ECMs are water, proteins and polysaccharides. However, their composition, architecture and bioactivity greatly vary from tissue to tissue in relation to the specific role the ECM is required to assume. This book overviews the role of the ECM in different tissues and organs of the human body.
Author: Robert Mecham
Publisher: Springer Science & Business Media
ISBN: 3642165559
Category : Science
Languages : en
Pages : 429
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Book Description
Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.
Author: Jaroslava Halper
Publisher: Springer Science & Business Media
ISBN: 9400778937
Category : Medical
Languages : en
Pages : 246
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Book Description
This volume is a reference handbook focusing on diseases like Marfan syndrome, Ehlers-Danlos syndrome, Loeys-Dietz syndrome and other heritable soft connective tissue diseases. The book presents detailed information for both basic scientists and for clinicians seeing patients. It is also a stepping stone for new investigations and studies that goes beyond the facts about the composition and biochemistry of the connective tissue and extracellular matrix, as the authors connect individual components to specific aspects of various soft tissue disorders and to the actual or potential treatment of them. Progress in Heritable Soft Connective Tissue Diseases features very prominent physicians and scientists as contributors who bring their most recent discoveries to the benefit of readers. Their expertise will help clinicians with proper diagnosis of sometimes elusive and uncommon heritable diseases of soft connective tissues. This book also offers an update on the pathophysiology of these diseases, including an emphasis on unifying aspects such as connections between embryonic development of the different types of connective tissues and systems, and the role of TGF-beta in development and physiology of soft tissues. This new set of data explains, at least in part, why many of these disorders are interconnected, though the primary pathophysiological events, such as gene mutations, may be different for each disorder.
Author: Irit Sagi
Publisher: Humana Press
ISBN: 9781493990948
Category : Science
Languages : en
Pages : 254
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Book Description
This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.
Author: David A.D. Parry
Publisher: Springer
ISBN: 3319496743
Category : Science
Languages : en
Pages : 630
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Book Description
This book provides the readers with an up-to-date review of the design, structure and function of a representative selection of fibrous proteins in both health and disease. The importance of the α-helical coiled coil, a conformational motif based on the heptad repeat in the amino acid sequence of all α-fibrous proteins (and parts of some globular proteins) is underlined by three Chapters devoted to its design, structure, function and topology. Specific proteins covered in the text and which depend on the coiled coil for their structure and function, include the intermediate filament proteins, tropomyosin, myosin, paramyosin, fibrin and members of the spectrin superfamily. Also described are fibrous proteins based on the β-pleated sheet and collagen conformations. Recombinant structural proteins, especially of silk and collagen, are discussed in the context of developing new biomaterials with varied applications. Established researchers and postgraduate students in the fields of protein chemistry, biochemistry and structural biophysics will find Fibrous Proteins: Structures and Mechanisms to be an invaluable collection of topical reviews that describe the basic advances made in the field of fibrous proteins over the past decade. This book, written by recognized authorities in the field, provides a clear account of the current status of fibrous protein research and, in addition, establishes the basis for deciding the most appropriate directions for future activity, including the applications of protein engineering and the commercial exploitation of new biomaterials.
Author: Peter D. Yurchenco
Publisher: Academic Press
ISBN: 1483289427
Category : Science
Languages : en
Pages : 483
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Book Description
The complex and critical process of extracellular matrix (ECM) assembly is described in this book. Assembly may involve molecules interacting with molecules of the same matrix class, such as in collagen, or interactions between different ECM molecules, such as in basement membranes. The text shows how this is driven by structural information within the matrix monomer. This information will be of interest to cell, developmental, and molecular biologists, biochemists, biophysicists, and biomedical researchers involved in macromolecular assembly, biological macromolecules, and extracellular matrix. - Addresses assembly of most of the known classes of extracellular matrix macromolecules - Discusses higher order structures produced by ECM - Gives important concepts in ECM and cell-matrix interactions, Protein structure and protein-protein interactions, Development and tissue remodeling
Author: Kursad Turksen
Publisher: Humana Press
ISBN: 9781493976553
Category : Science
Languages : en
Pages :
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Book Description
This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.
Author: Virginie Brun
Publisher: Humana Press
ISBN: 9781493991631
Category : Science
Languages : en
Pages : 293
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Book Description
This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Richard O. Hynes
Publisher:
ISBN: 9781936113385
Category : Cell interaction
Languages : en
Pages : 0
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Book Description
In most tissues, cells are surrounded by an extracellular matrix (ECM) containing proteins such as collagen, laminin, and fibronectin. The ECM plays an important role in regulating cell function. ECM proteins bind to integrins and other cell surface receptors, activating signaling pathways that regulate cellular morphology, adhesion, cell migration, cell proliferation, and apoptosis. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers all aspects of ECM composition and function, as well as alterations in the ECM that occur during development, tumorigenesis, and other disease states. The contributors examine the various ECM proteins and proteoglycans, ECM receptors such as integrins, and the signaling pathways that mediate the effects of the ECM on cells. They also describe ECM functions in specific biological contexts, including angiogenesis, hemostasis, and thrombosis. Covering not only the biochemistry and cell biology of the ECM but also its roles in development, physiology, and pathology, this volume is an indispensable reference for cell biologists and all those interested in exploring the myriad functions of the ECM.
