Engineering Periodontal Tissue Regeneration with the Use of a Novel Periostin Electrospun Scaffold PDF Download
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Author: Kendal I Creber
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Clinical therapies for the treatment of periodontitis are unable to reproducibly stimulate regeneration of the periodontium. We assessed the use of a novel electrospun type I collagen scaffold containing recombinant periostin to stimulate regeneration of the periodontal ligament (PDL) and bone. Human PDL cells demonstrated the ability to form a mineralized matrix in vitro and reduced osteogenic potential with increased donor age. In vitro analysis indicated scaffolds were biocompatible, however, periostin did not significantly influence adhesion or growth. In the healing of fenestration defects in rats, type I collagen scaffolds (with and without periostin) initially delayed cell infiltration and increased M2 macrophage recruitment, indicating either an elevated healing response or foreign body reaction. The incorporation of periostin into scaffolds had a limited effect on inflammation, collagen synthesis, and bone formation in vivo. Further optimization of periostin concentration and/or scaffold design is required to determine whether recombinant periostin influences periodontal healing.
Author: Kendal I Creber
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Clinical therapies for the treatment of periodontitis are unable to reproducibly stimulate regeneration of the periodontium. We assessed the use of a novel electrospun type I collagen scaffold containing recombinant periostin to stimulate regeneration of the periodontal ligament (PDL) and bone. Human PDL cells demonstrated the ability to form a mineralized matrix in vitro and reduced osteogenic potential with increased donor age. In vitro analysis indicated scaffolds were biocompatible, however, periostin did not significantly influence adhesion or growth. In the healing of fenestration defects in rats, type I collagen scaffolds (with and without periostin) initially delayed cell infiltration and increased M2 macrophage recruitment, indicating either an elevated healing response or foreign body reaction. The incorporation of periostin into scaffolds had a limited effect on inflammation, collagen synthesis, and bone formation in vivo. Further optimization of periostin concentration and/or scaffold design is required to determine whether recombinant periostin influences periodontal healing.
Author: Gayathri.K ,Sunanda Rao.K, P.L.Ravishankar
Publisher: BFC Publications
ISBN: 9363706281
Category : Medical
Languages : en
Pages : 87
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Book Description
The concept of scaffolds in tissue engineering has revolutionized the approach to periodontal therapy. This book delves into the various types of scaffolds, their biological and mechanical properties, and the innovative materials being utilized to enhance their effectiveness. This book addresses the integration of scaffold technology with other regenerative modalities such as growth factors, stem cells, and gene therapy. The synergistic effects of these combined approaches hold immense promise for the future of periodontal regeneration. This book covers topics related to the biological and mechanical aspects of scaffold use in regeneration .
Author: Rachel J. Waddington
Publisher: John Wiley & Sons
ISBN: 1118741072
Category : Medical
Languages : en
Pages : 418
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Book Description
Tissue Engineering and Regeneration in Dentistry: Current Strategies presents a thorough update on the current advances, methods and understanding in tissue engineering in dentistry. It offers invaluable tools, case studies, and methodologies for undertaking research, including important biological and practical considerations to facilitate successful migration of research from the bench to the clinic. Offers detailed coverage of the basic underlying principles and scientific evidence, and includes protocols to highlight practical applications Written by an internationally renowned team of expert contributors A must-have read for researchers and specialist clinicians in tissue engineering, oral biology, dental materials science, periodontology and oral surgery
Author: Elashri Chatterjee
Publisher: GRIN Verlag
ISBN: 3668711380
Category : Science
Languages : en
Pages : 131
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Book Description
Literature Review from the year 2016 in the subject Biology - Miscellaneous, grade: 1, , course: MASTERS OF DENTAL SURGERY, language: English, abstract: Tissue engineering applies the principles of Biology, Chemistry, Physics, and Engineering for the development of substitutes that replace, repair or enhance the biological function of diseased and damaged human body parts, by manipulating cells via their extracellular microenvironment. This three dimensional extracellular architecture ("scaffold") can be fabricated in the shape of the tissue we want to restore, with the help of either polymer hydrogel, self-assembly, non- woven matrix, nano-fibrous electrospun matrices, 3D weaving, or any other textile technology-based techniques, depending upon their structural and functional requirements. This concept in periodontics began with guided tissue regeneration, a mechanical approach utilizing non-resorbable membranes to regenerate periodontal defects. In dental implantology, guided bone regeneration membranes, with or without mechanical support, are used for bone augmentation.
Author: Giovanna Orsini
Publisher: Frontiers Media SA
ISBN: 2889199843
Category : Physiology
Languages : en
Pages : 248
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Book Description
Sequential and reciprocal interactions between oral epithelial and cranial neural crest-derived mesenchymal cells give rise to the teeth and periodontium. Teeth are vital organs containing a rich number of blood vessels and nerve fibers within the dental pulp and periodontium. Teeth are composed by unique and specific collagenous (dentin, fibrillar cementum) and non-collagenous (enamel) highly mineralized extracellular matrices. Alveolar bone is another collagenous hard tissue that supports tooth stability and function through its close interaction with the periodontal ligament. Dental hard tissues are often damaged after infection or traumatic injuries that lead to the partial or complete destruction of the functional dental and supportive tissues. Well-established protocols are routinely used in dental clinics for the restoration or replacement of the damaged tooth and alveolar bone areas. Recent progress in the fields of cell biology, tissue engineering, and nanotechnology offers promising opportunities to repair damaged or missing dental tissues. Indeed, pulp and periodontal tissue regeneration is progressing rapidly with the application of stem cells, biodegradable scaffolds, and growth factors. Furthermore, methods that enable partial dental hard tissue repair and regeneration are being evaluated with variable degrees of success. However, these cell-based therapies are still incipient and many issues need to be addressed before any clinical application. The understanding of tooth and periodontal tissues formation would be beneficial for improving regenerative attempts in dental clinics. In the present e-book we have covered the various aspects dealing with dental and periodontal tissues physiology and regeneration in 6 chapters: 1. General principles on the use of stem cells for regenerating craniofacial and dental tissues 2. The roles of nerves, vessels and stem cell niches in tissue regeneration 3. Dental pulp regeneration and mechanisms of various odontoblast functions 4. Dental root and periodontal physiology, pathology and regeneration 5. Physiology and regeneration of the bone using various scaffolds and stem cell populations 6. Physiology, pathology and regeneration of enamel using dental epithelial stem cells
Author: Parisa Pourattar
Publisher:
ISBN: 9781369060201
Category : Chitosan
Languages : en
Pages : 37
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Book Description
Periodontitis is a major chronic inflammatory disorder that can lead to the destruction of the periodontal tissues and, ultimately, tooth loss. It is a major cause of tooth loss in adults and a substantial public-health burden worldwide. There is thus a significant need for periodontal ligament (PDL) regeneration to enable functional mechanical support of tooth prostheses and prevent occlusal overloading. The goal of stem cell-based dental tissue engineering, is to create tooth-like structures using scaffold materials to guide the dental stem cells. Current resorbable membranes act as an epithelial tissue down-growth into the defect, favoring the regeneration of periodontal tissues. In order to develop synthetic grafts for these applications, different biocompatible materials have been used to fabricate fibers with different structures and morphologies. This study demonstrated the feasibility of using a composite material that combines the advantage of multiple materials to synthesize polyvinyl alcohol/ chitosan blend fiber scaffolds to promote PDL regeneration and to achieve a synthetic composite that match the native PDL modulus. Morphology, dispersibility, and mechanical properties of blend nanofibrous mats were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and tensile test.
Author: Elham Saberian
Publisher: BoD – Books on Demand
ISBN: 0850140218
Category :
Languages : en
Pages : 68
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Book Description
Author: Mona Marei
Publisher: Morgan & Claypool Publishers
ISBN: 160845214X
Category : Technology & Engineering
Languages : en
Pages : 178
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Book Description
Dental caries, periodontitis, tooth loss, and bone resorption are considered prevalent health problems that have direct affect on the quality of life. While, advances in stem cell biology and biotechnology have sparked hope for devastating maladies, such as diabetes, cardiovascular diseases, etc., it also provides a strategy of regenerative therapy for dental tissues. From the prospective of tissue engineering, it is of utmost importance to understand and emulate the complex cell interactions that make up a tissue or organ. Unlike other tissues in the body, dental tissues are unique in their development, function, and even in their maintenance throughout life. The harmonized stimulations of biology and mechanical regulators to promote cellular activities have matured our understanding of the value of regenerative therapy of dental tissue versus the reparative treatment. In this book, we review the current knowledge available to regenerate alveolar bone, periodontal structure, and pulp/dentin complex. The book provides researchers with detailed information about development and functional characteristics of the dental unit with detailed protocols covering a comprehensive range of various approaches to engineer dental tissues: to use isolated cells or cell substitutes as cellular replacement, to use acellular biomaterials capable of inducing tissue regeneration, and/or to use a combination of cells, biomaterial and growth factors. We are well aware, with the concept changes in the field toward in-vitro biomimetics of in-vivo tissue development. The theoretical frame work integrating these concepts of developmental biology and developmental engineering is yet to be emphasized and implemented. Until this happens, we consider this book of regenerative dentistry as a call for scientists to achieve, researchers to innovate, practitioners to apply, and students to learn the art and science of regenerative therapy in dentistry. Table of Contents: Introduction to Regenerative Dentistry / Tissue Engineering Alveolar Bone / Tissue Engineering of the Periodontal Tissues / Dynamics for Pulp-Dentin Tissue Engineering in Operative Dentistry
Author: Chamila Prage Panduwawala Kankanamalage
Publisher: Open Dissertation Press
ISBN: 9781361040126
Category : Medical
Languages : en
Pages : 228
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Book Description
This dissertation, "Assessment of Cell Sheet Constructs With Periodontal Ligament Stem Cells and Endothelial Cells for Periodontal Regeneration" by Chamila Prageeth Pandula, Panduwawala Kankanamalage, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Periodontitis is an infectious and inflammatory oral disease, which damages the tooth supporting structures and is one of the major causes of tooth loss in adults. Thus, periodontal diseases hinder the patient's quality of life in terms of oral health and functions leading to significant socio-economic outbreak worldwide. Since the periodontium has extremely limited and restricted capability for self-renewal, conventional treatments have not been influential in creating significant outcomes. These limitations have opened pathways for searching new approaches for periodontal regeneration. In recent years, periodontal ligament stem cells (PDLSCs) have been considered to be the promising cell source for periodontal regeneration in cell-based tissue engineering approaches. PDLSCs have been experimentally used for the regeneration of periodontal tissues and eventually demonstrated significant results in general. Cell sheet technology steps into tissue engineering and regenerative medicine as a novel approach by overcoming many drawbacks coupled with traditional tissue engineering methods. Using this approach, cells could be harvested as an intact cell sheet by adjusting the dish surface temperature. In this study, PDLSCs and human umbilical vein endothelial cells (HUVECs) were used to create three-dimensional (3-D) cell sheet constructs to assess the periodontal regeneration potential in vitro and in vivo. PDLSCs were characterized for their "stemness" and multilineage differentiation potentials. PDLSCs and HUVECs were used to create 3-D cell sheet constructs and unraveled the synergistic effects in different ratios of these cells. The present study suggested that co-culture groups exhibited higher level of osteo/odontogenic gene expressions compared to monoculture cell sheet groups. Moreover, vessel-like structures were observed in a cell sheet assembly in vitro. Further studies would be necessary to evaluate the exact parameters required for the development of such vessel-like structures and angiogenesis in 3-D cell sheet constructs in vitro. In vivo assessment of tooth-root coated with three different combinations of triple-layered cell sheet groups (PDLSCs-PDLSCs-PDLSCs, PDLSCs-HUVECs-PDLSCs and Co-culture group) showed periodontal ligament like tissue regeneration around implanted roots compared with control groups. Positive immunohistochemical staining for periostin and bone sialoprotein further confirmed periodontal regeneration, cementogenesis, and osteogenesis in cell sheet groups. Immunohistochemistry for human mitochondrial antibody was used to verify the cells of human origin. Vessel-like structures were observed in periodontal compartment of transplanted cell sheet groups (PDLSCs-HUVECs-PDLSCs and co-culture) facilitating the successful integration of host vasculature with transplanted cell sheet constructs. In summary, the results suggested that HUVECs enhanced the osteo/odontogenic potentials of PDLSCs in 3-D cell sheet model with formation of rudimentary vessel-like structures in vitro. Furthermore, following the transplantation of these cell sheet constructs with tooth roots into the subcutaneous space of immunodeficient mice, periodontal ligament like tissues were observed around transplanted constructs with the positive sign of cementogenesis and osteogenesis. In translational terms
Author: Elena Irina Paşcu
Publisher:
ISBN:
Category :
Languages : en
Pages : 218
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Book Description
