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Author: Judith Klinman
Publisher: Springer
ISBN: 3642389627
Category : Science
Languages : en
Pages : 217
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Book Description
Christopher M. Cheatum and Amnon Kohen, Relationship of Femtosecond–Picosecond Dynamics to Enzyme-Catalyzed H-Transfer. Cindy Schulenburg and Donald Hilvert, Protein Conformational Disorder and Enzyme Catalysis. A. Joshua Wand, Veronica R. Moorman and Kyle W. Harpole, A Surprising Role for Conformational Entropy in Protein Function. Travis P. Schrank, James O. Wrabl and Vincent J. Hilser, Conformational Heterogeneity Within the LID Domain Mediates Substrate Binding to Escherichia coli Adenylate Kinase: Function Follows Fluctuations. Buyong Ma and Ruth Nussinov, Structured Crowding and Its Effects on Enzyme Catalysis. Michael D. Daily, Haibo Yu, George N. Phillips Jr and Qiang Cui, Allosteric Activation Transitions in Enzymes and Biomolecular Motors: Insights from Atomistic and Coarse-Grained Simulations. Karunesh Arora and Charles L. Brooks III, Multiple Intermediates, Diverse Conformations, and Cooperative Conformational Changes Underlie the Catalytic Hydride Transfer Reaction of Dihydrofolate Reductase. Steven D. Schwartz, Protein Dynamics and the Enzymatic Reaction Coordinate.
Author: Judith Klinman
Publisher: Springer
ISBN: 3642389627
Category : Science
Languages : en
Pages : 217
Get Book Here
Book Description
Christopher M. Cheatum and Amnon Kohen, Relationship of Femtosecond–Picosecond Dynamics to Enzyme-Catalyzed H-Transfer. Cindy Schulenburg and Donald Hilvert, Protein Conformational Disorder and Enzyme Catalysis. A. Joshua Wand, Veronica R. Moorman and Kyle W. Harpole, A Surprising Role for Conformational Entropy in Protein Function. Travis P. Schrank, James O. Wrabl and Vincent J. Hilser, Conformational Heterogeneity Within the LID Domain Mediates Substrate Binding to Escherichia coli Adenylate Kinase: Function Follows Fluctuations. Buyong Ma and Ruth Nussinov, Structured Crowding and Its Effects on Enzyme Catalysis. Michael D. Daily, Haibo Yu, George N. Phillips Jr and Qiang Cui, Allosteric Activation Transitions in Enzymes and Biomolecular Motors: Insights from Atomistic and Coarse-Grained Simulations. Karunesh Arora and Charles L. Brooks III, Multiple Intermediates, Diverse Conformations, and Cooperative Conformational Changes Underlie the Catalytic Hydride Transfer Reaction of Dihydrofolate Reductase. Steven D. Schwartz, Protein Dynamics and the Enzymatic Reaction Coordinate.
Author: A. Cooper
Publisher: Springer Science & Business Media
ISBN: 1475716079
Category : Science
Languages : en
Pages : 479
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Book Description
This volume represents the proceedings of a NATO Advanced Studies Instituteheld near Barga (Italy), July 11-23, 1988, involving over 90 participants from more than twelve countries of Europe, North America and elsewhere. It was not our intention at this meeting to present a complete up-to-the-minute review of current research in enzyme catalysis but t·ather, in accord wi th the intended spiri t of NATO ASis, to gi ve an opportunity for advanced students and researchers in a wide variety of disciplines to meet tagether and study the problern from different points of view. Hence the lectures cover topics rauging from the purely theoretical aspects of chemical reaction kinetics in condensed matter through practical experimental approaches to enzyme structure, dynamics and mechanism, including the new experimental opportunities arising from genetic engineering techniques. Our approachwas unashamedly physical, both because the more biochemical aspects of enzymology are amply covered elsewhere and because progress in our understanding and application of the molecular basis of enzymic processes must ultimately come from advances in physical knowledge. We tried to cover as wide a spectrum as possible, and succeeded in gathering an expert and enthusiastic team of speakers, but there . are some inevitable omissions. In particular, and with hindsight, our discussions might have been enriched by more detailed coverage of general aspects of chemical catalysis - but readers requiring this background should find adequate references herein.
Author: Gorden Hammes
Publisher: Elsevier
ISBN: 032315817X
Category : Science
Languages : en
Pages : 276
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Book Description
Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry.
Author: Gertz I. Likhtenshtein
Publisher: Springer Science & Business Media
ISBN: 0306481103
Category : Science
Languages : en
Pages : 239
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Book Description
"This book is a view of enzyme catalysis by a physico-chemist with long-term experience in the investigation of structure and action mechanism of biological catalysts. This book is not intended to provide an exhaustive survey of each topic but rather a discussion of their theoretical and experimental background, and recent developments. The literature of enzyme catalysis is so vast and many scientists have made important contribution in the area, that it is impossible in the space allowed for this book to give a representative set of references. The author has tried to use reviews, and general principles of articles. He apologizes to those he has not been able to include. . ... The monograph is intended for scientists working on enzyme catalysis and adjacent areas such as chemical modeling of biological processes, homogeneous catalysis, biomedical research and biotechnology. The book can be use as a subsidiary manual for instructors, graduate and undergraduate students of university biochemistry and chemistry departments."--Pages ix-x.
Author: Sudhir P. Singh
Publisher: Elsevier
ISBN: 0128198214
Category : Technology & Engineering
Languages : en
Pages : 481
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Book Description
Advances in Enzyme Catalysis and Technologies intends to provide the basic structural and functional descriptions, and classification of enzymes. The scientific information related to the recombinant enzyme modifications, discovery of novel enzymes and development of synthetic enzymes are also presented. The translational aspects of enzyme catalysis and bioprocess technologies are illustrated, by emphasizing the current requirements and future perspectives of industrial biotechnology. Several case studies are included on enzymes for biofuels application, micro algal biorefineries, high-value bioactive molecules production and enzymes for environmental processes, such as enzymatic bioprocessing for functional food development, biocatalytic technologies for the production of functional sweetener, etc. Provides a conceptual understanding of enzyme catalysis, enzyme engineering, discovery of novel enzymes, and technology perspectives Includes comprehensive information about the inventions and advancement in enzyme system development for biomass processing and functional food developmental aspects Gives an updated reference for education and understanding of enzyme technology
Author: J.R. Exequiel T. Pineda
Publisher:
ISBN:
Category :
Languages : en
Pages : 172
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Book Description
Author: Yao Fan
Publisher:
ISBN:
Category : Catalysis
Languages : en
Pages : 358
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Book Description
Author: Gira Bhabha
Publisher:
ISBN:
Category : Molecules
Languages : en
Pages : 532
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Book Description
Conformational dynamics are important for a variety of biological functions, including enzyme catalysis. Many questions remain as to the role of protein dynamics in enzyme catalysis, and these questions are best addressed in model systems for which detailed kinetic, biological and biophysical data can be obtained. Dihydrofolate reductase (DHFR) is an enzyme present in almost all cell types, and catalyzes the stereospecific reduction of dihydrofolate (DHF) to tetrahydrofolate (THF) using NADPH as a cofactor. THF is a precursor for thymidylate synthesis and is, therefore, required for cell proliferation. Much biochemical and structural work has shed light on various aspects of E. coli DHFR (ecDHFR), making it a paradigm for understanding enzyme mechanism. We have studied the protein conformational motions present in human DHFR (hDHFR), and find that despite a high level of structural conservation between hDHFR and ecDHFR, the dynamic mechanisms of the two enzymes are quite different. While E. coli DHFR employs an extensive loop motion to facilitate ligand flux, its human counterpart has evolved a more subtle and sophisticated twisting-hinge motion to the same end. Based on the differences in flexibility and motion in these two enzymes, we designed a mutant of ecDHFR, which abolishes the active site dynamics without perturbing the protein structure or preorganization of the active site configuration. This mutant allowed us to assess the role of dynamics in ecDHFR catalysis, and we found that the millisecond timescale dynamics play a critical role in the chemical step of catalysis as well as in ligand flux for ecDHFR. Combining these detailed structural studies on ecDHFR and hDHFR with bioinformatic analyses, mutagenesis, x-ray and NMR data, we are able to gain insights into the evolution of DHFR dynamic mechanisms at an atomic level.
Author: Paul F. Cook
Publisher: Garland Science
ISBN: 1136844287
Category : Medical
Languages : en
Pages : 416
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Book Description
Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.
Author: H. Eggerer
Publisher: Springer Science & Business Media
ISBN: 3642817386
Category : Science
Languages : en
Pages : 221
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Book Description
Enzymes perform the executive role in growth, energy conversion, and repair of a living organism. Their activity is adjusted to their en vironment within the cell, being turned off, switched on, or finely tuned by specific metabolites according to demands at the physiologi cal level. Each enzyme discovered in the long history of enzymology has revealed its own individuality. Even closely related members of a family differ in specificity, stability or regulatory properties. Despite these, at first sight overwhelming aspects of individuality, common factors of enzymic reactions have been recognized. Enzymes are stereospecific catalysts even when a nonspecific process would yield the same product. Knowledge of the detailed stereochemistry of an enzymic reaction helps to deduce reaction mechanisms and to ob tain insight into the specific binding of substrates at the active site. This binding close to catalytically competent groups is related to the enormous speed of enzyme-catalyzed reactions. The physical ba sis of rate-enhancement is understood in principle and further exploit ed in the design of small organic receptor molecules as model enzymes. These aspects of enzyme catalysis are discussed in Session 1. Session 2 emphasizes the dynamic aspects of enzyme substrate inter action. Substrate must diffuse from solution space to the enzyme's surface. This process is influenced and can be greatly facilitated by certain electrostatic propterties of enzymes. The dynamic events during catalysis are studied by relaxation kinetics or NMR techniques.
