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Author: Pierre Taberlet
Publisher: Oxford University Press
ISBN: 0191079995
Category : Science
Languages : en
Pages : 272
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Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Author: Pierre Taberlet
Publisher: Oxford University Press
ISBN: 0191079995
Category : Science
Languages : en
Pages : 272
Get Book Here
Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Author:
Publisher: Elsevier
ISBN: 0128114320
Category : Medical
Languages : en
Pages : 3421
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Book Description
Encyclopedia of Bioinformatics and Computational Biology: ABC of Bioinformatics, Three Volume Set combines elements of computer science, information technology, mathematics, statistics and biotechnology, providing the methodology and in silico solutions to mine biological data and processes. The book covers Theory, Topics and Applications, with a special focus on Integrative –omics and Systems Biology. The theoretical, methodological underpinnings of BCB, including phylogeny are covered, as are more current areas of focus, such as translational bioinformatics, cheminformatics, and environmental informatics. Finally, Applications provide guidance for commonly asked questions. This major reference work spans basic and cutting-edge methodologies authored by leaders in the field, providing an invaluable resource for students, scientists, professionals in research institutes, and a broad swath of researchers in biotechnology and the biomedical and pharmaceutical industries. Brings together information from computer science, information technology, mathematics, statistics and biotechnology Written and reviewed by leading experts in the field, providing a unique and authoritative resource Focuses on the main theoretical and methodological concepts before expanding on specific topics and applications Includes interactive images, multimedia tools and crosslinking to further resources and databases
Author: Claude Amiard-Triquet
Publisher: Academic Press
ISBN: 0128011769
Category : Science
Languages : en
Pages : 519
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Book Description
Aquatic Ecotoxicology: Advancing Tools for Dealing with Emerging Risks presents a thorough look at recent advances in aquatic ecotoxicology and their application in assessing the risk of well-known and emerging environmental contaminants. This essential reference, brought together by leading experts in the field, guides users through existing and novel approaches to environmental risk assessment, then presenting recent advances in the field of ecotoxicology, including omics-based technologies, biomarkers, and reference species. The book then demonstrates how these advances can be used to design and perform assays to discover the toxicological endpoints of emerging risks within the aquatic environment, such as nanomaterials, personal care products, PFOS and chemical mixtures. The text is an invaluable reference for any scientist who studies the effects of contaminants on organisms that live within aquatic environments. - Provides the latest perspectives on emerging toxic risks to aquatic environments, such as nanomaterials, pharmaceuticals, chemical mixtures, and perfluorooctane sulfonate (PFOS) - Offers practical guidance on recent advances to help in choosing the most appropriate toxicological assay - Presents case studies and information on a variety of reference species to help put the ecotoxicological theory into practical risk assess
Author: Urusa Thaenkham
Publisher: Springer Nature
ISBN: 9811917868
Category : Science
Languages : en
Pages : 404
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Book Description
This book aims to provide fundamental knowledge and information for research in molecular systematics on parasitic helminths (nematode, trematode, cestode). The shreds of evidence of molecular systematics studies will be compiled and discussed in terms of the utilities and pitfalls of the genetic marker used for various purposes, which have been implemented for molecular systematics of parasitic nematodes, cestodes, and trematodes. Moreover, this book will also provide the procedure for research on molecular systematics and DNA taxonomy as the guideline to explore parasitic helminths. Finally, the further perspectives of utilizing genetic markers for molecular studies on parasitic helminths will be addressed in the context of applications from the laboratory to fieldwork such as DNA barcoding and environmental DNA metabarcoding of parasitic helminths. The book will benefit postgraduate students and researchers requiring the detailed knowledge of molecular systematics, as well as researchers desiring a guideline to select genetic markers and analyze DNA sequences to make phylogenetic inferences
Author: Pierre Taberlet
Publisher: Oxford University Press
ISBN: 0198767226
Category : Science
Languages : en
Pages : 268
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Book Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functionalgenes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is nowa rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using adiversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmentalDNA.
Author: Frith Jarrad
Publisher: CABI
ISBN: 1780643594
Category : Science
Languages : en
Pages : 388
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Book Description
This book is a source of information on practical and innovative approaches to biosecurity surveillance. It explains the foundation and concepts behind surveillance design, with examples of methods and tools created to deal with surveillance challenges. With supporting case studies and including current directions in research, it covers evidence-based approaches to surveillance, statistics, detectability, single and multi-species detection, risk assessment, diagnostics, data-basing, modelling of invasion and spread, optimisation, and future climate challenges.
Author: Jerney, Jacqueline
Publisher: Nordic Council of Ministers
ISBN: 9289375191
Category : Science
Languages : en
Pages : 68
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Book Description
We present guidelines for using environmental DNA metabarcoding, together with conventional techniques, to monitor the taxonomic diversity of phytoplankton in marine and brackish waters. The focus is on eukaryotic and prokaryotic phytoplankton using 18S and 16S rRNA primers and high-throughput sequencing. Information and recommendations on sampling, sample processing, molecular biological work, quality control, bioinformatics, data storage and management and cost estimates are included so that the method can be used to complement standardized light microscopy. A scientific literature review, discussion on future perspectives, reference databases and standardization are included. Using eDNA metabarcoding to complement standardized light microscopy advances conventional monitoring and research on phytoplankton communities to assess biodiversity and the state of the marine environment.
Author: Graham Rowe
Publisher: Oxford University Press
ISBN: 0198716990
Category : Nature
Languages : en
Pages : 547
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Book Description
Revised edition of: Introduction to molecular ecology / Trevor J. C. Beebee, Graham Rowe. 2008. 2nd ed.
Author: Meissner, Kristian
Publisher: Nordic Council of Ministers
ISBN: 9289368063
Category : Business & Economics
Languages : en
Pages : 101
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Book Description
Available online: https://pub.norden.org/temanord2020-538/ Over 75% of all lakes and 40% of all rivers in the EU are found in the Nordic countries. Biomonitoring according to the EU Water Framework Directive (WFD) is adopted by all Nordic countries and forms an integral part of management efforts to preserve and restore the ecological quality of freshwaters and their ecosystem services. Organisms used in WFD biomonitoring are identified by experts; this is time consuming and error prone. Molecular identification methods could be used but have not been tested for monitoring. We compared molecular to expert identification for samples from 297 Nordic lakes and streams. The DNA-based results were highly similar to expert identifications. We suggest that Nordic or European efforts towards implementation and standardization of DNA-based methodology should be undertaken to swiftly ensure the use of this promising tool in future WFD monitoring.
Author: Pilar Rodriguez
Publisher: Springer Science & Business Media
ISBN: 9400717180
Category : Science
Languages : en
Pages : 271
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Book Description
In aquatic ecosystems, the oligochaetes are often a major component of the community. Their relevance in sediment quality assessment is largely related to their benthic and detritivorous life habit. In this book, we aim to present the state of the art of Pollution Biology using oligochaete worms in laboratory and field studies. Future research will require the combination of a variety of methodological approaches and the integration of the resulting information, avoiding fragmented and often conflicting visions of the relationships of the species with their environment. Current approaches to ecotoxicology and bioaccumulation using ecological risk assessment provide the opportunity to relate community studies with probability of effects. This book addresses three main themes: Ecological and Field Studies using the composition and structure of oligochaete communities, Toxicology and Laboratory Studies, and Bioaccumulation and Trophic Transfer Studies. Two appendices list values of toxicological parameters (LC50, EC50) and several bioaccumulation variables (bioaccumulation factors, biological half-life, toxicokinetic coefficients, and critical body residues) for different oligochaete species. Additional information is provided on Methodological Issues and on the Taxonomy of several oligochaete families, with information on the most recent taxonomic debates. Each chapter includes a critical view, based on the authors’ experience, of a number of current issues which have been raised in the literature.
