DNA Cloning: Mammalian systems. High efficiency gene transfer into mammalian cells PDF Download
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Author: David M. Glover
Publisher:
ISBN:
Category : Molecular cloning
Languages : en
Pages :
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Book Description
Author: David M. Glover
Publisher:
ISBN:
Category : Molecular cloning
Languages : en
Pages :
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Book Description
Author: William C. Heiser
Publisher: Springer Science & Business Media
ISBN: 1592596495
Category : Science
Languages : en
Pages : 303
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Book Description
Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Author: S.C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Category : Science
Languages : en
Pages : 730
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Book Description
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.
Author: William C. Heiser
Publisher: Humana Press
ISBN: 9781588290953
Category : Science
Languages : en
Pages : 566
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Book Description
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.
Author: R. Kucherlapati
Publisher: Springer Science & Business Media
ISBN: 1468451677
Category : Medical
Languages : en
Pages : 447
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Book Description
Genetic analysis of microbial systems provided us with the foundation for un derstanding gene structure, expression, and regulation. It was long felt that the ability to generate mutants and conduct genetic studies in mammalian systems would prove to be equally useful. However, genetic analysis based on sexual systems is difficult in mammals because of the long generation times and the inability to perform controlled matings. As a result, genetic analysis of mam malian systems had to await the development of parasexual systems. This book is an attempt to bring together descriptions of a number of these parasexual systems. A common theme of all the parasexual systems is the transfer of genetic information from a defined source into a specific cell type. This volume deals with a number of methods of gene transfer into mammalian cells. The early methods of gene transfer involved transfer of relatively large amounts of genetic information. These include somatic cell hybridization, microcell fusion, and chromosome transfer, which constitute the first part of this book. Each of these methods has already proven to be of enormous value in arriving at a genetic understanding of the mammalian genome. Development of recombinant DNA methods, and the ability to introduce purified DNA into mammalian cells, has had a significant impact on our ability to dissect important aspects of mammalian gene expression and regulation. The second part of this book deals with gene transfer systems involving defined nucleic acid sequences.
Author: David M. Glover
Publisher: Oxford University Press, USA
ISBN: 9780199634811
Category : Biology
Languages : en
Pages : 352
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Book Description
This book is one of four which together are considerably updated and totally revised texts that arose out of the very successful "DNA Cloning, Volume I-III" books in the Practical Approach series. This particular book describes ... techniques for genetic manipulation of mammalian cells, including gene amplification, retroviral and adenoviral vectors, and production of transgenic animals.
Author: David M. Glover
Publisher:
ISBN: 9780947946180
Category : Cloning
Languages : en
Pages : 208
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Book Description
V.1 - The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries; Constructing and screening cDNA libraries in g10 and gt11; An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors; Immunological detection of chimeric B-galactosidases expressed by plasmid vectors; The pEMBL family of single-stranded vectors; Techniques for transformation of E. coli; The use of genetic markers for the selection and allelic exchange of in vitro induced mutation that do not have a phenotype in E. coli; Tle oligonucleotide-directed construction of mutations in recombinant filamentous phage; broad host range cloning vectors for gram negative bacteria; v.2 - Bacillus cloning methods; gene cloning in streptomyces; Cloning in yeast; Genetic engineering of plants; P element mediated germ line transformation of drosophila; High efficiency gene transfer into mammalian cells; The construction and characterization of vaccina virus recombinants expressing foreign genes; Bovine papilloma virus DNA: a eukaryotic cloning vector.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309076374
Category : Science
Languages : en
Pages : 295
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Book Description
Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
Author: Jamie Davies
Publisher:
ISBN: 019884154X
Category :
Languages : en
Pages : 137
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Book Description
Written primarily for mid-to-upper level undergraduates, this primer will introduce students to topics at the forefront of the subject that are being applied to probe biological problems, or to address the most pressing issues facing society. These topics will include those that form thecornerstone of contemporary research, helping students to make the transition to active researcher.This primer introduces the challenges and opportunities of applying synthetic biological techniques to mammalian cells, tissues, and organisms. It covers the special features that make engineering mammalian systems different from engineering bacteria, fungi, and plants, and provides an overview ofcurrent techniques. A variety of cutting-edge examples illustrate the different purposes of mammalian synthetic biology, including pure biomedical research, drug production, tissue engineering, and regenerative medicine.
Author: Richard Twyman
Publisher: Garland Science
ISBN: 0203489233
Category : Science
Languages : en
Pages : 299
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Book Description
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
