Development of Matrix-assisted Laser Desorption/ionization and Liquid Chromatography - Electrospray Ionization Based Mass Spectrometric Techniques for Characterizing and Quantifying Endogenous and Therapeutic Biomolecules PDF Download
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Author: Bingming Chen
Publisher:
ISBN:
Category :
Languages : en
Pages : 463
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Book Description
Endogenous biomolecules, such as neuropeptide, protein and carbohydrates, play important roles in biological and physiological processes. Over the last two decades, therapeutic biomolecules, including therapeutic peptides, proteins and monoclonal antibodies, have become large and fast-growing segments of the pharmaceutical industry. However, analyzing biomolecules is challenging due to their large size, complex structure and various modifications. In situ analysis of biomolecules is even more challenging due to the large dynamic range and complex microenvironments on tissue surface. In this dissertation, matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography (LC) -- electrospray ionization (ESI) based mass spectrometric techniques were developed to characterize and quantify endogenous and therapeutic biomolecules. Specifically, this work developed novel ionization techniques and mass spectrometry imaging workflows to study the sequence and in situ distribution of biomolecules on high resolution accurate mass instrument platforms. Moreover, absolute or relative quantifications of biomolecules, such as therapeutic peptide or N-glycans, were measured by cutting-edge quantification workflows. In summary, this work not only improves current analytical methodology for characterizing biomolecules, but also demonstrates its potential applications in pharmaceutical industry and clinical settings.
Author: Bingming Chen
Publisher:
ISBN:
Category :
Languages : en
Pages : 463
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Book Description
Endogenous biomolecules, such as neuropeptide, protein and carbohydrates, play important roles in biological and physiological processes. Over the last two decades, therapeutic biomolecules, including therapeutic peptides, proteins and monoclonal antibodies, have become large and fast-growing segments of the pharmaceutical industry. However, analyzing biomolecules is challenging due to their large size, complex structure and various modifications. In situ analysis of biomolecules is even more challenging due to the large dynamic range and complex microenvironments on tissue surface. In this dissertation, matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography (LC) -- electrospray ionization (ESI) based mass spectrometric techniques were developed to characterize and quantify endogenous and therapeutic biomolecules. Specifically, this work developed novel ionization techniques and mass spectrometry imaging workflows to study the sequence and in situ distribution of biomolecules on high resolution accurate mass instrument platforms. Moreover, absolute or relative quantifications of biomolecules, such as therapeutic peptide or N-glycans, were measured by cutting-edge quantification workflows. In summary, this work not only improves current analytical methodology for characterizing biomolecules, but also demonstrates its potential applications in pharmaceutical industry and clinical settings.
Author: Richard B. Cole
Publisher: John Wiley & Sons
ISBN: 1118211553
Category : Science
Languages : en
Pages : 900
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Book Description
Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Author: Rainer Cramer
Publisher: Springer
ISBN: 3319048198
Category : Science
Languages : en
Pages : 287
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Book Description
This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics
Author: Kim Chung Lee
Publisher:
ISBN:
Category : Electrospray ionization mass spectrometry
Languages : en
Pages : 380
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Book Description
Author: Bojidarka Ivanova
Publisher: GRIN Verlag
ISBN: 3668703167
Category : Science
Languages : en
Pages : 89
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Book Description
Research Paper (postgraduate) from the year 2018 in the subject Chemistry - Analytical Chemistry, grade: Mass spectrometry, University of Dortmund (Institut für Umweltforschung, Lehrstuhl für Analytische Chemie), language: English, abstract: The goal of this work is to describe more recent developments in the quantitative mass spectrometry and to illustrate how our new model equations based on the stochastic dynamics relate to the determining the 3D molecular and electronic structures of analytes. It is aimed at researchers in Chemistry who would like to find out about what is going on in mass spectrometric methodological contributions more recently. The work could also be used to MSc and PhD students in the field of Chemistry, in particular, highlighting the ‘Analytical chemistry’, ‘Physico–chemistry’ and/or ‘Computational and theoretical chemistry’, respectively.
Author: Richard B. Cole
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 610
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Book Description
Comprehensive, up-to-date coverage of a revolutionary technique Electrospray ionization mass spectrometry (ESI-MS) has completely changed the way physicists, chemists, and biologists view the study of large molecules. The technique derives detailed information about molecular weights and structures from extremely small sample quantities. ESI-MS can create highly charged forms of very high molecular weight compounds, it is naturally compatible with many types of separation techniques, and it allows noncovalent interactions between molecules in solution to be preserved in the gas phase. But many researchers may not use the technique to its full potential because they are unfamiliar with the different perspectives of its underlying processes, the varied approaches to implementation, and the wide-ranging utility of the technique. In this book, Richard B. Cole and an assemblage of leading researchers present a single-volume compilation of different approaches to the understanding and exploitation of ESI-MS. This comprehensive guide: * Examines the physical and chemical aspects of the electrospray process and describes the events involved in ion formation as well as the electro-chemical phenomena that are central to charged droplet formation during the process * Explores the coupling of electrospray ionization to various mass spectrometers, including quadrupole, magnetic, time-of-flight, quadrupole ion trap, and Fourier transform ion cyclotron resonance instruments * Describes recent progress in interfacing ESI with solution-based separation techniques, including liquid chromatography and capillary electrophoresis * Charts the rapid development of ESI applications and categorizes them by compound type: peptides and proteins, nucleic acids and their constituents, carbohydrates and lipids, small molecules related to pharmacology and drug metabolism, and organometallics and inorganic compounds Electrospray Ionization Mass Spectrometry is the indispensable handbook and reference for anyone who wishes to understand, implement, or apply this technique, including researchers in chemistry, metallochemistry, biochemistry, biology, pharmacology, and physics.
Author: Walter A. Korfmacher
Publisher: John Wiley & Sons
ISBN: 1118516141
Category : Science
Languages : en
Pages : 686
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Book Description
Facilitates the discovery and development of new, effective therapeutics With coverage of the latest mass spectrometry technology, this book explains how mass spectrometry can be used to enhance almost all phases of drug discovery and drug development, including new and emerging applications. The book's fifteen chapters have been written by leading pharmaceutical and analytical scientists. Their contributions are based on a thorough review of the current literature as well as their own experience developing new mass spectrometry techniques to improve the ability to discover and develop new and effective therapeutics. Mass Spectrometry for Drug Discovery and Drug Development begins with an overview of the types of mass spectrometers that facilitate drug discovery and development. Next it covers: HPLC high-resolution mass spectrometry for quantitative assays Mass spectrometry for siRNA Quantitative analysis of peptides Mass spectrometry analysis of biological drugs Applications that support medicinal chemistry investigations Mass spectrometry imaging and profiling Throughout the book, detailed examples underscore the growing role of mass spectrometry throughout the drug discovery and development process. In addition, images of mass spectra are provided to explain how results are interpreted. Extensive references at the end of each chapter guide readers to the primary literature in the field. Mass Spectrometry for Drug Discovery and Drug Development is recommended for readers in pharmaceutics, including medicinal chemists, analytical chemists, and drug metabolism scientists. All readers will discover how mass spectrometry can streamline and advance new drug discovery and development efforts.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
The field of mass spectrometry has grown tremendously over the past few decades due in large part to the continued application to new and interesting areas of exploration. The advent of soft ionization sources such as electrospray ionization and matrix-assisted laser desorption/ionization have dramatically increased the capabilities of mass spectrometry and increased the amount of information derived from biological samples. As a result, there has been tremendous growth in the development of new ionization source technology in recent years. One of the main driving forces behind the development of new ionization technology is for the reduction of sample preparation required prior to analysis, yielding high throughput sample analysis. Demonstrated herein is the development and characterization of an atmospheric pressure ionization source called matrix-assisted laser desorption electrospray ionization (MALDESI). MALDESI is a hybrid combination of MALDI and ESI which utilizes laser desorption with electrospray postionization for the generation of multiply-charged ions. Multiply-charged ions are of particular importance when using Fourier transform mass spectrometry, due to the increase in resolving power and mass accuracy with increasing charge on the molecule. Top-down characterization of intact polypeptides is demonstrated as well as high mass accuracy utilizing internal calibration. A newly designed highly robust and versatile atmospheric pressure ionization platform is developed and described in detail. Solid- and liquid state analysis of three out of the four classes of biological molecules is demonstrated utilizing the newly developed versatile ionization platform. MALDESI utilizing ultraviolet and infrared laser desorption is demonstrated at various wavelengths (UV, 337 nm and 349 nm and IR, 2.94 Ã'Âæm and 10.6 Ã'Âæm) with and without ESI postionization for the generation of multiply-charged ions. The characteristics of liquid-state samples are described as a mac.
Author: Salvatore Fanali
Publisher: Elsevier
ISBN: 0128093447
Category : Science
Languages : en
Pages : 840
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Book Description
Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Author: Kei Zaitsu
Publisher: Elsevier
ISBN: 0128172215
Category : Science
Languages : en
Pages : 294
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Book Description
Ambient Ionization Mass Spectrometry in Life Sciences: Principles and Applications is a systematic introduction to this rapidly expanding area of study. Underlying principles of each technique are explained in detail, along with discussions on their applications across life science disciplines. Ambient ionization has recently emerged as one of the hottest and fastest growing topics in mass spectrometry, hence this book is not just for analysts and researchers who use and study mass spectrometry. This volume would be of interest to anyone who works in or studies analytical chemistry, omics sciences (including metabolomics), pharmacokinetics, forensic science or drug analysis. - Covers the most up-to-date techniques, including DART, DCBI, DESI, PESI, PSI, REIMS and laser-based ambient ionization - Includes easy-to-understand pros and cons of each ionization technique to aid in decision-making - Provides plentiful examples of life science applications
