Development of Efficient Regenration Protocols for Agrobacterium Mediated Gene Transfer in Brassica Oleracea Var. Botrytis PDF Download
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Author: Nidhi Arora
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Nidhi Arora
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Kan Wang
Publisher: Springer Science & Business Media
ISBN: 1597451304
Category : Science
Languages : en
Pages : 474
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Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Author: Seyed Ali Ravanfar
Publisher:
ISBN:
Category : Agrobacterium
Languages : en
Pages : 314
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![Development of Efficient Protocols for Plant Regeneration and Agrobacterium-mediated Transformation in Orka (Abelmoschus Esculentus (L.) Moench) [With CD Copy]. PDF](https://books.google.com/books/content/images/frontcover/lB7VYgEACAAJ?fife=w80-h100)
Author: Apoorva Arora
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Renate Schmidt
Publisher: Springer Science & Business Media
ISBN: 1441971181
Category : Science
Languages : en
Pages : 675
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The Genetics and Genomics of the Brassicaceae provides a review of this important family (commonly termed the mustard family, or Cruciferae). The family contains several cultivated species, including radish, rocket, watercress, wasabi and horseradish, in addition to the vegetable and oil crops of the Brassica genus. There are numerous further species with great potential for exploitation in 21st century agriculture, particularly as sources of bioactive chemicals. These opportunities are reviewed, in the context of the Brassicaceae in agriculture. More detailed descriptions are provided of the genetics of the cultivated Brassica crops, including both the species producing most of the brassica vegetable crops (B. rapa and B. oleracea) and the principal species producing oilseed crops (B. napus and B. juncea). The Brassicaceae also include important “model” plant species. Most prominent is Arabidopsis thaliana, the first plant species to have its genome sequenced. Natural genetic variation is reviewed for A. thaliana, as are the genetics of the closely related A. lyrata and of the genus Capsella. Self incompatibility is widespread in the Brassicaceae, and this subject is reviewed. Interest arising from both the commercial value of crop species of the Brassicaceae and the importance of Arabidopsis thaliana as a model species, has led to the development of numerous resources to support research. These are reviewed, including germplasm and genomic library resources, and resources for reverse genetics, metabolomics, bioinformatics and transformation. Molecular studies of the genomes of species of the Brassicaceae revealed extensive genome duplication, indicative of multiple polyploidy events during evolution. In some species, such as Brassica napus, there is evidence of multiple rounds of polyploidy during its relatively recent evolution, thus the Brassicaceae represent an excellent model system for the study of the impacts of polyploidy and the subsequent process of diploidisation, whereby the genome stabilises. Sequence-level characterization of the genomes of Arabidopsis thaliana and Brassica rapa are presented, along with summaries of comparative studies conducted at both linkage map and sequence level, and analysis of the structural and functional evolution of resynthesised polyploids, along with a description of the phylogeny and karyotype evolution of the Brassicaceae. Finally, some perspectives of the editors are presented. These focus upon the Brassicaceae species as models for studying genome evolution following polyploidy, the impact of advances in genome sequencing technology, prospects for future transcriptome analysis and upcoming model systems.
Author: Alejandra Mora-Avilʹes
Publisher:
ISBN:
Category : Agrobacterium tumefaciens
Languages : en
Pages : 474
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"The control of pathogens in food crops is a general concern around the world. Much effort has been devoted to the control of fungal pathogens, mainly by pesticide application, cultural practices, biocontrol, or traditional plant breeding. Limited sources of resistance among cultivated plants or their wild relatives have made it important to look for alternative resistance genes and transfer techniques. This dissertation addresses the introduction of a Trichoderma. harzianum endochitinase gene into Brassica oleracea and B. napus by Agrobacterium tumefaciens-mediated transformation, and the molecular analysis and fungal resistance of the transgenic plants. The T. harzianum endochitinase has been reported to have fungal inhibiting properties in assays performed in vitro. Recently, transgenic crops such as potato, tomato, tobacco, apple, and grape expressing the endochitinase gene have been analyzed to assess their protection against fungal pathogens, with variable results. Chapter I reviews previous reports of chitinase genes from plants and microorganisms used in plant transformation. It describes the levels of expression of chitinase in various transgenic plants and the levels of control of fungal pathogens. Chapter I also discusses previous work on Agrobacterium-mediated transformation of Brassica oleracea. Chapter II presents experiments testing some of the factors that affect the efficiency of Agrobacterium-mediated transformation of broccoli. Chapter III focuses on molecular aspects of gene expression of transgenic broccoli and rapeseed plants containing the T. harzianum endochitinase gene and describes pathogenic assays to assess the resistance of the transgenic plants to the fungal pathogens Alternaria brassicicola and Sclerotinia sclerotiorum. Chapter IV discusses the interaction of endochitinase and the fungicide Bayleton for the control of A. brassicicola. Finally, Chapter V addresses the induction of pathogenesis-related proteins by constitutive expression of the endochitinase gene"--Leaves xv-xvi
Author: Ian S. Curtis
Publisher: Springer Science & Business Media
ISBN: 1402023332
Category : Technology & Engineering
Languages : en
Pages : 448
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Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.
Author: Shabir Hussain Wani
Publisher: Springer Nature
ISBN: 3030346943
Category : Technology & Engineering
Languages : en
Pages : 261
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Global population is mounting at an alarming stride to surpass 9.3 billion by 2050, whereas simultaneously the agricultural productivity is gravely affected by climate changes resulting in increased biotic and abiotic stresses. The genus Brassica belongs to the mustard family whose members are known as cruciferous vegetables, cabbages or mustard plants. Rapeseed-mustard is world’s third most important source of edible oil after soybean and oil palm. It has worldwide acceptance owing to its rare combination of health promoting factors. It has very low levels of saturated fatty acids which make it the healthiest edible oil that is commonly available. Apart from this, it is rich in antioxidants by virtue of tocopherols and phytosterols presence in the oil. The high omega 3 content reduces the risk of atherosclerosis/heart attack. Conventional breeding methods have met with limited success in Brassica because yield and stress resilience are polygenic traits and are greatly influenced by environment. Therefore, it is imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying yield, quality and tolerance towards biotic and abiotic stresses in Brassica. To exploit its fullest potential, systematic efforts are needed to unlock the genetic information for new germplasms that tolerate initial and terminal state heat coupled with moisture stress. For instance, wild relatives may be exploited in developing introgressed and resynthesized lines with desirable attributes. Exploitation of heterosis is another important area which can be achieved by introducing transgenics to raise stable CMS lines. Doubled haploid breeding and marker assisted selection should be employed along with conventional breeding. Breeding programmes aim at enhancing resource use efficiency, especially nutrient and water as well as adoption to aberrant environmental changes should also be considered. Biotechnological interventions are essential for altering the biosynthetic pathways for developing high oleic and low linolenic lines. Accordingly, tools such as microspore and ovule culture, embryo rescue, isolation of trait specific genes especially for aphid, Sclerotinia and alternaria blight resistance, etc. along with identification of potential lines based on genetic diversity can assist ongoing breeding programmes. In this book, we highlight the recent molecular, genetic and genomic interventions made to achieve crop improvement in terms of yield increase, quality and stress tolerance in Brassica, with a special emphasis in Rapeseed-mustard.
Author: Naseem Ahmad
Publisher: Springer Nature
ISBN: 981159046X
Category : Science
Languages : en
Pages : 339
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Plant tissue culture (PTC) technology has gained unassailable success for its various commercial and research applications in plant sciences. Plant growth regulators (PGRs) are an essential part of any plant tissue culture intervention for propagation or modification of plants. A wide range of PGRs are available, including aromatic compounds that show cytokinin activities, promote cell division and micro-propagation, viz. kinetin, N6-benzyladenine and topolins. Topolins are naturally occurring aromatic compounds that have gained popularity as an effective alternative for other frequently used cytokinins in in vitro culture of plants. Among them, meta-topolin [6-(3-hydroxybenzlyamino) purine] is the most popular and its use in plant tissue culture has amplified swiftly. During the last few decades, there have been numerous reports highlighting the effectiveness of meta-topolin in micropropagation and alleviation of various physiological disorders, rooting and acclimatization of tissue culture raised plants.
Author: Edwin F. George
Publisher:
ISBN: 9780950932545
Category : Plant micropropagation
Languages : en
Pages :
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