Development of a Rapid, Immobilized Probe Assay for the Detection of Mitochondrial DNA Variation in the Hvi and Hvii Regions - Scholar's Choice Edition PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Development of a Rapid, Immobilized Probe Assay for the Detection of Mitochondrial DNA Variation in the Hvi and Hvii Regions - Scholar's Choice Edition PDF full book. Access full book title Development of a Rapid, Immobilized Probe Assay for the Detection of Mitochondrial DNA Variation in the Hvi and Hvii Regions - Scholar's Choice Edition by Cassandra D Calloway. Download full books in PDF and EPUB format.
Author: Cassandra D Calloway
Publisher: Scholar's Choice
ISBN: 9781297047909
Category :
Languages : en
Pages : 54
Get Book Here
Book Description
This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work.As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
Author: Cassandra D Calloway
Publisher: Scholar's Choice
ISBN: 9781297047909
Category :
Languages : en
Pages : 54
Get Book Here
Book Description
This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work.As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
Author: Cassandra D. Calloway
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 0
Get Book Here
Book Description
Author: R. Reynolds
Publisher:
ISBN:
Category : DNA Typing--Human
Languages : en
Pages : 22
Get Book Here
Book Description
We have developed a rapid, immobilized probe-based assay for the detection of sequence variation in the hyper-variable segment II (HVII) of the mitochondrial DNA (mtDNA) control region. Using a panel of 17 sequence-specific oligonucleotide (SSO) probes immobilized on nylon membrane strips, we typed 689 individuals from four population groups. The genetic diversity value for each population was calculated from the frequency data, and the frequencies of distinct "mitotypes" in each group were determined. We performed DNA sequence analysis of 129 samples to characterize the sequences associated with "blanks" (absence of probe signals) and weak probe signals. Out of 689 samples, we observed five heteroplasmic samples (excluding the variable C-stretch beginning at position 303) using the immobilized SSO probe panel. The SSO probe strips were used for the analysis of shed hairs and bloodstains from several criminal cases in Sweden, one of which is described here. We conclude that this mtDNA typing system is useful for human identification and significantly decreases casework turnaround time.
Author: MC. Kline
Publisher:
ISBN:
Category : DNA typing
Languages : en
Pages : 9
Get Book Here
Book Description
Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. The primary advantage of mtDNA is that it is present in high copy number within cells and therefore more likely to be recovered from highly degraded specimens. A major disadvantage to traditional forensic mtDNA analysis is that it is time-consuming and labor-intensive to generate and review the 610 nucleotides of sequence information commonly targeted in hypervariable regions I and II (HVI and HVII) of the control region. In addition, common haplotypes exist in HVI/HVII mtDNA sequences that can reduce the ability to differentiate two unrelated samples. In this report we describe the utility of two newly available screening assays for rapid exclusion of non-matching samples. The LINEAR ARRAY mtDNA HVI/HVII Region-Sequencing Typing Kit (Roche Applied Science, Indianapolis, IN) was used to type 666 individuals from U.S. Caucasian, African American, and Hispanic groups. Processing of the LINEAR ARRAY probe panels "mito strips" was automated on a ProfiBlot workstation. Observable variation in 666 individuals is reported and frequencies of the mitotypes within and between populations are presented. Samples exhibiting the most common Caucasian mitotype were subdivided with a multiplexed amplification and detection assay using eleven single nucleotide polymorphisms in the mitochondrial genome. These types of screening assays should enable more rapid evaluation of forensic casework samples such that only samples not excluded would be subjected to further characterization through full HVI/HVII mtDNA sequence analysis.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages :
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1568
Get Book Here
Book Description
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Author: Bolanle Ariyo
Publisher:
ISBN:
Category :
Languages : en
Pages : 92
Get Book Here
Book Description
Author: Cassandra Taylor
Publisher:
ISBN: 9781369616446
Category :
Languages : en
Pages :
Get Book Here
Book Description
Forensic DNA samples are often highly degraded, making them unsuitable for traditional methods of DNA analysis, such as STR analysis, because target sequences and primer binding sites are not intact. Mitochondrial DNA (mtDNA) is useful for analyzing degraded DNA because of its high copy number; the high copy number makes successful typing more likely. Traditional methods of mtDNA analysis, including Sanger sequencing, are commonly used to only target only the HVI/HVII regions of the mitochondrial genome. These analysis methods miss discriminating information outside of the HV1/HVII, limiting the discriminatory power of mitochondrial DNA analysis. Probe capture is a novel technique that uses mtDNA sequence-specific probes to enrich and capture the entire mitochondrial genome. Analyzing the entire mitochondrial genome allows detection of discriminating information outside of the HVI/HVII regions and increases the discriminatory power of mitochondrial DNA analysis. Next Generation Sequencing, a massively parallel, clonal, and high-throughput technique, is an excellent tool for analyzing the entire mitochondrial genome. The purpose of this project was to test the efficiency of Dr. Calloway’s optimized probe capture assay on degraded DNA by analyzing the entire mitochondrial genome of highly degraded bone samples. Throughout the course of this project, three sets of bones samples, dating to 100, 2000, and 4000 years old respectively, were tested. Seven highly degraded bone samples recovered from a comingled tomb in Rijeka, Croatia and dating to approximately 100 years old were successfully sequenced with coverage of the mitochondrial genome ranging from approximately 52 to 98%. Haplogroups were determined for six of these samples based on the sequenced variants present in the entire mitochondrial genome. Additionally, six prehistoric bone samples dating to approximately 2000 years ago were sequenced with coverage of the mitochondrial genome ranging from 26 to 100%. The last set of samples, dating to 4000 years old, was recovered from a necropolis on the island of Kor?ula, Croatia. These six samples were successfully sequenced with coverage of the mitochondrial genome ranging from 26 to 100%.
Author: National Institute of Justice (U.S.)
Publisher:
ISBN:
Category : DNA fingerprinting
Languages : en
Pages : 164
Get Book Here
Book Description
Author: Lawrence Kobilinsky
Publisher: John Wiley & Sons
ISBN: 111806223X
Category : Science
Languages : en
Pages : 544
Get Book Here
Book Description
A concise, robust introduction to the various topics covered by the discipline of forensic chemistry The Forensic Chemistry Handbook focuses on topics in each of the major chemistry-related areas of forensic science. With chapter authors that span the forensic chemistry field, this book exposes readers to the state of the art on subjects such as serology (including blood, semen, and saliva), DNA/molecular biology, explosives and ballistics, toxicology, pharmacology, instrumental analysis, arson investigation, and various other types of chemical residue analysis. In addition, the Forensic Chemistry Handbook: Covers forensic chemistry in a clear, concise, and authoritative way Brings together in one volume the key topics in forensics where chemistry plays an important role, such as blood analysis, drug analysis, urine analysis, and DNA analysis Explains how to use analytical instruments to analyze crime scene evidence Contains numerous charts, illustrations, graphs, and tables to give quick access to pertinent information Media focus on high-profile trials like those of Scott Peterson or Kobe Bryant have peaked a growing interest in the fascinating subject of forensic chemistry. For those readers who want to understand the mechanisms of reactions used in laboratories to piece together crime scenes—and to fully grasp the chemistry behind it—this book is a must-have.
