Design of a Versatile and Disposable Microfluidic Chip for Automated Sample Preparation and Nucleic Acid Extraction PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Design of a Versatile and Disposable Microfluidic Chip for Automated Sample Preparation and Nucleic Acid Extraction PDF full book. Access full book title Design of a Versatile and Disposable Microfluidic Chip for Automated Sample Preparation and Nucleic Acid Extraction by John Glenn Minson. Download full books in PDF and EPUB format.
Author: John Glenn Minson
Publisher:
ISBN:
Category : Microfluidic devices
Languages : en
Pages : 58
Get Book Here
Book Description
Author: John Glenn Minson
Publisher:
ISBN:
Category : Microfluidic devices
Languages : en
Pages : 58
Get Book Here
Book Description
Author: Bhattacharjee, Sukanta
Publisher: River Publishers
ISBN: 8770220557
Category : Technology & Engineering
Languages : en
Pages : 182
Get Book Here
Book Description
Recent microfluidic technologies have brought a complete paradigm shift in automating biochemical processing on a tiny lab-on-chip (a.k.a. biochip) that replaces expensive and bulky instruments traditionally used in implementing bench-top laboratory protocols. Biochips have already made a profound impact on various application domains such as clinical diagnostics, DNA analysis, genetic engineering, and drug discovery, among others. They are capable of precisely manipulating micro-/pico-liter quantities of fluids, and provide integrated support for mixing, storage, transportation, and sensing, on-chip. In almost all bioprotocols, sample preparation plays an important role, which includes dilution and mixing of several fluids satisfying certain volumetric ratios. However, designing algorithms that minimize reactant-cost and sample-preparation time suited for microfluidic chips poses a great challenge from the perspective of protocol mapping, scheduling, and physical design. Algorithms for Sample Preparation with Microfluidic Lab-on-Chip attempts to bridge the widening gap between biologists and engineers by introducing, from the fundamentals, several state-of-the-art computer-aided-design (CAD) algorithms for sample preparation with digital and flow-based microfluidic biochips. Technical topics discussed in the book include: Basics of digital and flow-based microfluidic lab-on-chipComprehensive review of state-of-the-art sample preparation algorithmsSample-preparation algorithms for digital microfluidic lab-on-chipSample-preparation algorithms for flow-based microfluidic lab-on-chip
Author: Sudip Poddar
Publisher: CRC Press
ISBN: 1000612708
Category : Technology & Engineering
Languages : en
Pages : 223
Get Book Here
Book Description
Microfluidic biochips have gained prominence due to their versatile applications to biochemistry and health-care domains such as point-of-care clinical diagnosis of tropical and cardiovascular diseases, cancer, diabetes, toxicity analysis, and for the mitigation of the global HIV crisis, among others. Microfluidic Lab-on-Chips (LoCs) offer a convenient platform for emulating various fluidic operations in an automated fashion. However, because of the inherent uncertainty of fluidic operations, the outcome of biochemical experiments performed on-chip can be erroneous even if the chip is tested a priori and deemed to be defect-free. This book focuses on the issues encountered in reliable sample preparation with digital microfluidic biochips (DMFBs), particularly in an error-prone environment. It presents state-of-the-art error management techniques and underlying algorithmic challenges along with their comparative discussions. Describes a comprehensive framework for designing a robust and error-tolerant biomedical system which will help in migrating from cumbersome medical laboratory tasks to small-sized LOC-based systems Presents a comparative study on current error-tolerant strategies for robust sample preparation using DMFBs and reports on efficient algorithms for error-tolerant sample dilution using these devices Illustrates how algorithmic engineering, cyber-physical tools, and software techniques are helpful in implementing fault tolerance Covers the challenges associated with design automation for biochemical sample preparation Teaches how to implement biochemical protocols using software-controlled microfluidic biochips Interdisciplinary in its coverage, this reference is written for practitioners and researchers in biochemical, biomedical, electrical, computer, and mechanical engineering, especially those involved in LOC or bio-MEMS design.
Author: Anita Rogacs
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
This dissertation describes three techniques aimed at automated, sensitive sample preparation and detection of RNA from complex samples. Microfluidic systems have advanced the state of the art for a wide number of chemical and biological assays, but robust and efficient sample preparation remains a major challenge. One of the most difficult processes is the lysing, purification, and detection of target RNA from whole blood samples. This dissertation addresses key challenges in each of these RNA workflow phases. In the first part of the dissertation, we demonstrate a novel assay for lysing followed by physicochemical extraction and isotachophoresis-based purification of 16S ribosomal RNA from whole human blood infected with Pseudomonas Putida. This assay is unique in that the extraction can be automated on-chip using isotachophoresis in a simple device with no moving parts, it protects RNA from degradation when isolating from ribonuclease-rich matrices (like blood), and produces a purified total nucleic acid (NA) sample which is compatible with enzymatic amplification assays. We show that the purified RNA are compatible with reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and demonstrate a clinically relevant sensitivity of 0.03 bacteria per nanoliter using RT-qPCR. In the second part of the dissertation, we present a model to aid in design and optimization of a wide range of electrophoresis (including isotachophoresis) assays. Our model captures the important contributors to the effects of temperature on the observable electrophoretic mobilities of small ions, and on solution ionic strength, conductivity and pH; the most relevant parameters in molecular reactions and separation assays. Our temperature model includes relations for temperature-dependent viscosity, ionic strength corrections, degree of ionization (pK), and ion solvation effects on mobility. We incorporate thermophysical data for water viscosity; temperature-dependence of the Onsager-Fuoss model for finite ionic strength effects on mobility; temperature-dependence of the extended Debye-Huckel theory for correction of ionic activity; the Clarke-Glew approach and tabulated thermodynamic quantities of ionization reaction for acid dissociation constants as a function of temperature; and species-specific, empirically evaluated correction terms for temperature-dependence of Stokes' radii. We incorporated our model into a MATLAB-based simulation tool we named Simulation of Temperature Effects on ElectroPhoresis (STEEP). We validated our model using conductance and pH measurements across a temperature variation of 25°C to 70°C for a set of electrolytes routinely used in electrophoresis. The model accurately captures electrolyte solution pH and conductivity, including important effects not captured by simple Walden type relations. In the third and final part of the dissertation, we introduce a cost-effective and simple-to-implement method for direct detection of RNA, by analyzing images of randomly distributed multicolor fluorescent beads bound by the target molecule. We term our method particle imaging, tracking and collocation (PITC). We use a fairly standard epifluorescence microscopy setup fitted with an off-the-shelf dual view color separator attachment which images fluorescence emission at two wavelengths onto two respective halves of a single CCD image array. We perform automated analysis of these two color channels to track thousands of particle images in either or both wavelengths, and we track particle image motion in time and space. We can quantify particle image wavelength (or wavelength ratio), absolute intensities, and particle image diameter. We also perform cross-correlation image analyses on this multi-wavelength data to track particle collocations and the degree of correlation of particle motion. Particles or cells can be suspended in solution and flowed through a wide variety of microchannels (with optical access for image collection). Particles can be transported through the detection region via electrophoresis and/or pressure driven flow, to increase throughput of analysis. We here introduce and evaluate the performance of our method. We use Monte Carlo simulations to demonstrate robustness of the algorithm and optimize algorithm parameters. We present an experimental demonstration of the method on challenging image data, including flow of randomly distributed Brownian particles and particle populations which undergo particle-to-particle binding. We show results of bead collocation measurements on bead-to-bead binding created by the E.coli 16S rRNA gene.
Author: Zipeng Li
Publisher: Springer
ISBN: 3030029646
Category : Technology & Engineering
Languages : en
Pages : 152
Get Book Here
Book Description
This book provides an insightful guide to the design, testing and optimization of micro-electrode-dot-array (MEDA) digital microfluidic biochips. The authors focus on the characteristics specific for MEDA biochips, e.g., real-time sensing and advanced microfluidic operations like lamination mixing and droplet shape morphing. Readers will be enabled to enhance the automated design and use of MEDA and to develop a set of solutions to facilitate the full exploitation of design complexities that are possible with standard CMOS fabrication techniques. The book provides the first set of design automation and test techniques for MEDA biochips. The methods described in this book have been validated using fabricated MEDA biochips in the laboratory. Readers will benefit from an in-depth look at the MEDA platform and how to combine microfluidics with software, e.g., applying biomolecular protocols to software-controlled and cyberphysical microfluidic biochips.
Author: Jun Zeng
Publisher: Springer Science & Business Media
ISBN: 1402051239
Category : Technology & Engineering
Languages : en
Pages : 407
Get Book Here
Book Description
Design Automation Methods and Tools for Microfluidics-Based Biochips deals with all aspects of design automation for microfluidics-based biochips. Experts have contributed chapters on many aspects of biochip design automation. Topics covered include: device modeling; adaptation of bioassays for on-chip implementations; numerical methods and simulation tools; architectural synthesis, scheduling and binding of assay operations; physical design and module placement; fault modeling and testing; and reconfiguration methods.
Author: Fatima H Labeed
Publisher: Royal Society of Chemistry
ISBN: 1849737606
Category : Science
Languages : en
Pages : 295
Get Book Here
Book Description
The concept of a miniaturised laboratory on a disposable chip is now a reality, and in everyday use in industry, medicine and defence. New devices are launched all the time, prompting the need for a straightforward guide to the design and manufacture of lab-on-a-chip (LOC) devices. This book presents a modular approach to the construction and integration of LOC components in detection science. The editors have brought together some of the leading experts from academia and industry to present an accessible guide to the technology available and its potential. Several chapters are devoted to applications, presenting both the sampling regime and detection methods needed. Further chapters describe the integration of LOC devices, not only with each other but also into existing technologies. With insights into LOC applications, from biosensing to molecular and chemical analysis, and presenting scaled-down versions of existing technology alongside unique approaches that exploit the physics of the micro and nano-scale, this book will appeal to newcomers to the field and practitioners requiring a convenient reference.
Author: Shelley D. Minteer
Publisher: Springer Science & Business Media
ISBN: 1592599974
Category : Science
Languages : en
Pages : 245
Get Book Here
Book Description
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author: Jaime Castillo-León
Publisher: Springer
ISBN: 3319086871
Category : Technology & Engineering
Languages : en
Pages : 246
Get Book Here
Book Description
This book covers all the steps in order to fabricate a lab-on-a-chip device starting from the idea, the design, simulation, fabrication and final evaluation. Additionally, it includes basic theory on microfluidics essential to understand how fluids behave at such reduced scale. Examples of successful histories of lab-on-a-chip systems that made an impact in fields like biomedicine and life sciences are also provided. This book also: · Provides readers with a unique approach and toolset for lab-on-a-chip development in terms of materials, fabrication techniques, and components · Discusses novel materials and techniques, such as paper-based devices and synthesis of chemical compounds on-chip · Covers the four key aspects of development: basic theory, design, fabrication, and testing · Provides readers with a comprehensive list of the most important journals, blogs, forums, and conferences where microfluidics and lab-on-a-chip news, methods, techniques and challenges are presented and discussed, as well as a list of companies providing design and simulation support, components, and/or developing lab-on-a-chip and microfluidic devices.
Author: Krishnendu Chakrabarty
Publisher: CRC Press
ISBN: 1439819165
Category : Medical
Languages : en
Pages : 216
Get Book Here
Book Description
Microfluidics-based biochips combine electronics with biochemistry, providing access to new application areas in a wide variety of fields. Continued technological innovations are essential to assuring the future role of these chips in functional diversification in biotech, pharmaceuticals, and other industries.Revolutionary guidance on design, opti
