Design and Construction of Designer Bioester Libraries for Validation of the Modular Cell Theory PDF Download
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Author: Donovan Layton
Publisher:
ISBN:
Category : Biomass energy
Languages : en
Pages : 185
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Book Description
Renewable and sustainable fuels and chemicals are required for mankind to reduce their dependence on petroleum. Metabolic engineering and synthetic biology have provided avenues for production of renewable fuels and chemicals by using waste feedstocks derived from biomass, municipal, and off gases, such as carbon dioxide and methane, using microbial cell factories. However, development of optimal microbial cell factories has been a challenge due to the vast combinations of pathways, genetic parts, and hosts to produce a targeted product. The purpose of this work is for validation of the modular cell theory via rational pathway design and testing for development of optimal microbial cell factories. This dissertation is divided up into four different parts. Part I focuses on engineering and production of butyrate ester libraries for use as fuels, flavors, fragrances and solvents, specifically ethyl butyrate, isopropyl butyrate, and isobutyl butyrate using a modular chassis cell derived from the modular cell theory. Part II focuses on the synthesis of designer esters from waste organic acids, the carboxylates, as well as characterizing the enzyme responsible for condensing ester precursor molecules for novel activity using the modular chassis cell. Part III focuses on the expansion of part II by modulating an ester precursor molecule for the production of novel esters that can be used as next generation biofuels. Part IV focuses on further validating the modular cell theory by using growth-based selection for ethanol production by varying open reading frames and genetic parts. The work presented will validate and further provide insights to modular cell theory and ester biosynthesis from fermentable sugars and organic acids.
Author: Donovan Layton
Publisher:
ISBN:
Category : Biomass energy
Languages : en
Pages : 185
Get Book Here
Book Description
Renewable and sustainable fuels and chemicals are required for mankind to reduce their dependence on petroleum. Metabolic engineering and synthetic biology have provided avenues for production of renewable fuels and chemicals by using waste feedstocks derived from biomass, municipal, and off gases, such as carbon dioxide and methane, using microbial cell factories. However, development of optimal microbial cell factories has been a challenge due to the vast combinations of pathways, genetic parts, and hosts to produce a targeted product. The purpose of this work is for validation of the modular cell theory via rational pathway design and testing for development of optimal microbial cell factories. This dissertation is divided up into four different parts. Part I focuses on engineering and production of butyrate ester libraries for use as fuels, flavors, fragrances and solvents, specifically ethyl butyrate, isopropyl butyrate, and isobutyl butyrate using a modular chassis cell derived from the modular cell theory. Part II focuses on the synthesis of designer esters from waste organic acids, the carboxylates, as well as characterizing the enzyme responsible for condensing ester precursor molecules for novel activity using the modular chassis cell. Part III focuses on the expansion of part II by modulating an ester precursor molecule for the production of novel esters that can be used as next generation biofuels. Part IV focuses on further validating the modular cell theory by using growth-based selection for ethanol production by varying open reading frames and genetic parts. The work presented will validate and further provide insights to modular cell theory and ester biosynthesis from fermentable sugars and organic acids.
Author: Ari Koskinen
Publisher: Springer Science & Business Media
ISBN: 9780751402599
Category : Science
Languages : en
Pages : 338
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Book Description
The outlook of organic synthesis has changed many times during its tractable history. The initial focus on the synthesis of substances typical of living matter, exemplified by the first examples of organic chemistry through the synthesis of urea from inorganic substances by Liebig, was accepted as the birth of organic chemistry, and thus also of organic synthesis. Although the early developments in organic synthesis closely followed the pursuit of molecules typical in nature, towards the end of the 19th century, societal pressures placed higher demands on chemical methods appropriate for the emerging age of industrialization. This led to vast amounts of information being generated through the discovery of synthetic reactions, spectroscopic techniques and reaction mechanisms. The basic organic functional group transformations were discovered and improved during the early part of this century. Reaction mechanisms were elucidated at a growing pace, and extremely powerful spectroscopic tools, such as infrared, nuclear magnetic resonance and mass spectrometry were introduced as everyday tools for a practising organic chemist. By the 1950s, many practitioners were ready to agree that almost every molecule could be syn thesized. Some difficult stereochemical problems were exceptions; for example Woodward concluded that erythromycin was a "hopelessly complex target". This frustration led to a hectic phase of development of new and increasingly more ingenious protecting group strategies and functional group transformations, and also saw the emergence of asymmetric synthesis.
Author: Eric Gustafson
Publisher:
ISBN: 9781495129636
Category : Femap (Computer software)
Languages : en
Pages : 330
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Book Description
Author: Ioan Sarbu
Publisher: Academic Press
ISBN: 0128116633
Category : Technology & Engineering
Languages : en
Pages : 442
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Book Description
Solar Heating and Cooling Systems: Fundamentals, Experiments and Applications provides comprehensive coverage of this modern energy issue from both a scientific and technical level that is based on original research and the synthesis of consistent bibliographic material that meets the increasing need for modernization and greater energy efficiency to significantly reduce CO2 emissions. Ioan Sarbu and Calin Sebarchievici present a comprehensive overview of all major solar energy technologies, along with the fundamentals, experiments, and applications of solar heating and cooling systems. Technical, economic, and energy saving aspects related to design, modeling, and operation of these systems are also explored. This reference includes physical and mathematical concepts developed to make this publication a self-contained and up-to-date source of information for engineers, researchers, and professionals who are interested in the use of solar energy as an alternative energy source. - Includes learning aims, chapter summaries, problems and solutions to support the theories presented - Puts a specific emphasis on the practical application of the technologies in heating and cooling systems - Contains calculating equations for the energy and economic index of solar systems
Author: Erwin-Christian Lovasz
Publisher: Springer Nature
ISBN: 3030600769
Category : Technology & Engineering
Languages : en
Pages : 569
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Book Description
This volume gathers the proceedings of the Joint International Conference of the XIII International Conference on Mechanisms and Mechanical Transmissions (MTM) and the XXIV International Conference on Robotics (Robotics), held in Timişoara, Romania. It addresses the applications of mechanisms and transmissions in several modern technical fields such as mechatronics, biomechanics, machines, micromachines, robotics and apparatus. In doing so, it combines theoretical findings and experimental testing. The book presents peer-reviewed papers written by researchers specialized in mechanism analysis and synthesis, dynamics of mechanisms and machines, mechanical transmissions, biomechanics, precision mechanics, mechatronics, micromechanisms and microactuators, computational and experimental methods, CAD in mechanism and machine design, mechanical design of robot architecture, parallel robots, mobile robots, micro and nano robots, sensors and actuators in robotics, intelligent control systems, biomedical engineering, teleoperation, haptics, and virtual reality.
Author: Vipin Chandra Kalia
Publisher: Springer
ISBN: 3319526669
Category : Science
Languages : en
Pages : 335
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Book Description
This contributed volume sheds new light on waste management and the production of biofuels. The authors share insights into microbial applications to meet the challenges of environmental pollution and the ever- growing need for renewable energy. They also explain how healthy and balanced ecosystems can be created and maintained using strategies ranging from oil biodegration and detoxification of azo dyes to biofouling. In addition, the book illustrates how the metabolic abilities of microorganisms can be used in microbial fuel-cell technologies or for the production of biohydrogen. It inspires young researchers and experienced scientists in the field of microbiology to explore the application of green biotechnology for bioremediation and the production of energy, which will be one of the central topics for future generations.
Author: IEEE Staff
Publisher:
ISBN: 9781728129501
Category :
Languages : en
Pages :
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Book Description
The Internet of Things (IoT) technology offers unprecedented opportunities to interconnect human beings as well as Machine to Machine (M2M), whereby sensors and networks allow all things to communicate directly with each other to share vital information allowing us to have an instrumented universe where accurate data is readily available to inform optimal decision making The IoT is about to enable a range of new capabilities and services far beyond today s offerings The scale of the IoT is set to have a major economic, social and environmental impacts the intersection of which forms the future sustainable growth The IEEE Internet of Things Systems, Management and Security (IoTSMS 2019) aims at soliciting original ideas on the broad area of IoT including challenges and opportunities, concepts and applications and future trends
Author: Ioan Sarbu
Publisher: Academic Press
ISBN: 0128042419
Category : Technology & Engineering
Languages : en
Pages : 217
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Book Description
Ground-Source Heat Pumps presents the theory and some of the most recent advances of GSHPs and their implementation in the heating/cooling system of buildings. The authors explore the thermodynamic cycle with calculation, operation regimes and economic indicators and GHG emissions of a vapor compression heat pump. They go on to examine substitution strategies of non-ecological refrigerants and types of compressors and heat pumps, before delving into the different GSHP systems, as well as their compared economic, energy and environmental performances using classical and optimized adjustment for various operating modes. Surface water heat pumps and ground water heat pumps are covered, and special focus is given to both vertical and horizontal ground-coupled heat pump systems, for which modelling and simulation is discussed, and experimental systems are described. Due to its advanced approach to the subject, this book will be especially valuable for researchers, graduate students and academics, and as reference for engineers and specialists in the varied domains of building services. Explores fundamentals and state-of-the-art research, including ground-coupled heat pump (GCHP) systems. Includes performance assessment and comparison for different types of GSHP, numerical simulation models, practical applications of GSHPs with details on the renewable energy integration, information on refrigerants, and economic analysis.
Author: Anya Schiffrin
Publisher: The New Press
ISBN: 1595589732
Category : History
Languages : en
Pages : 322
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Book Description
Crusading journalists from Sinclair Lewis to Bob Woodward and Carl Bernstein have played a central role in American politics: checking abuses of power, revealing corporate misdeeds, and exposing government corruption. Muckraking journalism is part and parcel of American democracy. But how many people know about the role that muckraking has played around the world? This groundbreaking new book presents the most important examples of world-changing journalism, spanning one hundred years and every continent. Carefully curated by prominent international journalists working in Asia, Africa, Latin America, Europe, and the Middle East, Global Muckraking includes Ken Saro-Wiwa’s defense of the Ogoni people in the Niger Δ Horacio Verbitsky's uncovering of the gruesome disappearance of political detainees in Argentina; Gareth Jones’s coverage of the Ukraine famine of 1932–33; missionary newspapers’ coverage of Chinese foot binding in the nineteenth century; Dwarkanath Ganguli’s exposé of the British "coolie" trade in nineteenth-century Assam, India; and many others. Edited by the noted author and journalist Anya Schiffrin, Global Muckraking is a sweeping introduction to international journalism that has galvanized the world’s attention. In an era when human rights are in the spotlight and the fate of newspapers hangs in the balance, here is both a riveting read and a sweeping argument for why the world needs long-form investigative reporting.
Author: Roberto Mazzoli
Publisher: Frontiers Media SA
ISBN: 2889451852
Category :
Languages : en
Pages : 144
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Book Description
Microorganisms have had a long and surprising history. They were “invisible” until invention of microscope in the 17th century. Until that date, although they were extensively (but inconsciously) employed in food preservation, beer and wine fermentation, cheese, vinegar, yogurt and bread making, as well as being the causative agents of infectious diseases, they were considered as “not-existing”. The work of Pasteur in the middle of the 19th century revealed several biological activities performed by microorganisms including fermentations and pathogenicity. Due to the urgent issue to treat infectious diseases (the main cause of death at those times) the “positive potential” of the microbial world has been neglected for about one century. Once the fight against the “evil” strains was fulfilled also thanks to the antibiotics, industry began to appreciate bacteria’s beneficial characteristics and exploit selected strains as starters for both food fermentations and aroma, enzyme and texturing agent production. However, it was only at the end of the 20th century that the probiotic potential of some bacteria such as lactic acid bacteria and bifidobacteria was fully recognized. Very recently, apart from the probiotic activity of
in toto bacteria, attention has begun to be directed to the chemical mediators of the probiotic effect. Thanks also to the improvement of techniques such as transcriptomics, proteomics and metabolomics, several bioactive compounds are continuously being discovered. Bioactive molecules produced by bacteria, yeasts and virus-infected cells proved to be important for improving or impairing human health. The most important result of last years’ research concerns the discovery that a very complex network of signals allows communication between organisms (from intra-species interactions to inter-kingdom signaling). Based on these findings a completely new approach has arisen: the system biology standpoind. Actually, the different organisms colonizing a certain environmental niche are not merely interacting with each other as individuals but should be considered as a whole complex ecosystem continuously exchanging information at the molecular level. In this context, this topic issue explores both antagonistic compounds (i.e. antibiotics) and “multiple function” cooperative molecules improving the physiological status of both stimulators and targets of this network. From the applicative viewpoint, these molecules could be hopefully exploited to develop new pharmaceuticals and/or nutraceuticals for improving human health.
