Molecular Biology of The Cell PDF Download
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Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: Xiaowen Bai
Publisher: Nova Science Publishers
ISBN: 9781626187993
Category : Epidermis
Languages : en
Pages : 0
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Book Description
Dermal fibroblasts are the most numerous cells within the dermal layer of skin. The main function of fibroblasts is to preserve the structural integrity of skin through constantly secreting extracellular matrix. Various factors diversely affect the secretion activities of fibroblasts and may lead to varying disease processes (eg: solar damage and wrinkles). This book gathers important cutting-edge research in the field of fibroblasts covering characterisation of fibroblasts and their roles in multiple disease processes such as hypertrophic scarring, ageing/photoaging, diabetic wound healing, cancer, and pachydermoperiostosis. In addition, the chapters also deal with the stem cell-like properties of fibroblasts, which sheds new light on the commonly used definition of fibroblasts and provides novel insights into many past studies performed with fibroblast preparations, since the reported effects might be greatly attributable to stem cell content within these fibroblasts. Additionally, this book features an introduction of the reprogramming of dermal fibroblasts into patient-specific induced pluripotent stem cells (iPSCs) that can be converted into varying terminally differentiated cells. Application of iPSCs is a rapidly developing research field because reprogrammed iPSCs from dermal fibroblasts provides a great tool for studying molecular mechanisms of disease, development, and tissue regeneration. This book is certain to have broad appeal and should provide a useful resource for researchers, students, teachers, and physicians interested in the field.
Author: Sophie Böttcher-Haberzeth
Publisher: Springer Nature
ISBN: 1493994735
Category : Science
Languages : en
Pages : 260
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Book Description
This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.
Author: John Varga
Publisher: Springer Science & Business Media
ISBN: 1592599400
Category : Medical
Languages : en
Pages : 393
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Book Description
Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
This technical report outlines procedures that have been developed to provide a cost effective way to produce large quantities of normal human skin fibroblast cells (NHSFs) for studying the mechanism(s) of action of chemical warfare agents (CWAs) and medical counter-measurements against CWAs. Various growth factors were added to media recommended by the supplier, American Type Culture Collection (ATCC), Manassas, Virginia, USA. Cell viability, cell differentiation and cell proliferation were evaluated using different tissue culture assays in particular the CyQUANT(Registered) Cell Proliferation Assay kit obtained from Molecular Probes, Inc., Eugene, Oregon, USA. This particular assay measures cell proliferation and was used as indicated in the experimental protocol provided by the product brochure. We found cultured human skin cells to be a useful model for skin irritation testing.
Author: Marion Claudia Salzer
Publisher:
ISBN:
Category :
Languages : en
Pages : 207
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Book Description
"Aging is accompanied by cellular, extracellular and systemic changes that impair tissue function. Stromal alterations, such as degeneration of the extracellular matrix, are thought to contribute to organ deterioration in the elderly, yet little is known whether this is caused by changes in fibroblasts, the main cellular component of stroma. Using population- and single-cell whole transcriptomics, as well as long-term lineage tracing, we studied if and how murine dermal fibroblasts are altered during physiological aging. Old dermal fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix but, intriguingly, also gain adipogenic traits. Many of these age-related alterations are influenced by systemic changes in metabolism: long-term caloric restriction prevents them in old fibroblasts in a reversible manner, whereas a high-fat diet potentiates them in young fibroblasts. Single-cell RNA sequencing detects two fibroblast subtypes in adult dermis: one characterized by expression of collagens, and the other characterized by expression of genes involved in processes of oxidation-reduction and immune regulation. We show that the identity of these two populations becomes blurred with age, and that gene expression of old fibroblasts is noisy. Our results therefore highlight that identity-noise and the acquisition of adipogenic traits are mechanisms that accompany fibroblast aging, which is sensitive to changes in systemic metabolism." -- TDX.
Author: Robert Fitridge
Publisher: University of Adelaide Press
ISBN: 1922064009
Category : Medical
Languages : en
Pages : 589
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Book Description
New updated edition first published with Cambridge University Press. This new edition includes 29 chapters on topics as diverse as pathophysiology of atherosclerosis, vascular haemodynamics, haemostasis, thrombophilia and post-amputation pain syndromes.
Author: Adilson Da Costa
Publisher: Springer Nature
ISBN: 3319782657
Category : Medical
Languages : en
Pages : 817
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Book Description
Minimally invasive aesthetic procedures are an important part of dermatologists’ day-to-day clinical routine. However, plastic surgeons are also becoming more willing to explore them, and minimally invasive cosmetic and aesthetic procedures are now an established interdisciplinary topic. Minimally Invasive Aesthetic Procedures - A Guide for Dermatologists and Plastic Surgeons addresses the needs of both these specialties. It provides a comprehensive overview of the most relevant and widely used minimally invasive procedures, presented in a practical and straightforward style. Rather than a broad overview of the literature, it offers a step-by-step guide to clinical procedures. Each chapter explores a single clinical procedure, discussing the theoretical basis; the materials needed; the methods and techniques; clinical follow-up; before-and-after illustrations; as well as the side effects and complications and their management. It also includes a summary of tips and relevant references. With more than a hundred procedures presented and discussed in a clinically applicable format, Minimally Invasive Aesthetic Procedures - A Guide for Dermatologists and Plastic Surgeons is a practical manual for all dermatology and plastic surgery practitioners who are interested in aesthetic medicine.
Author: Yoshiharu Takayama
Publisher: Springer Science & Business Media
ISBN: 9400724675
Category : Medical
Languages : en
Pages : 118
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Book Description
Lactoferrin is an iron-binding glycoprotein belonging to the transferrin family. It acts as a defense in host animals against microbes and viruses, since it has a broad spectrum of antimicrobial and antiviral activities. Lactoferrin has been shown to regulate the growth and differentiation of many types of cells. The results of recent studies indicate that lactoferrin is a potent regulator of dermal fibroblasts, and promotes cutaneous wound healing. The collagen gel contraction, a model of wound contraction during wound healing process, and migration of human fibroblasts were enhanced by lactoferrin. LRP-1 (LDL Receptor related Protein-1) acts as a signaling receptor for lactoferrin that mediate fibroblast response to lactoferrin by activating ERK/MAPK signaling pathway. In addition, lactoferrin promotes biosynthesis of extracellular matrix (ECM) component such as type-I collagen and hyaluronan. Hyaluronan is a major component of ECM in connective tissue and promotes wound healing. The promoting effect of lactoferrin on hyaluronan production was accompanied by promotion of HAS2 (hyaluronan synthase 2) expression. These observations suggest that lactoferrin promotes the wound healing by providing an ECM that promotes fibroblast migration. Lactoferrin is also known for its anti-inflammatory and immune modulating properties. According to recent in vivo study, lactoferrin promotes wound repair by promoting the early inflammatory phase of wound healing. Based on this, recombinant human lactoferrin was subsequently tested clinically in a Phase II trial in patients with diabetic ulcers and was found to be effective. Lactoferrin should be further evaluated in patients with diabetic and other types of ulcers.
Author: Robert Lanza
Publisher: Elsevier
ISBN: 008053967X
Category : Science
Languages : en
Pages : 1039
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Book Description
The opportunity that tissue engineering provides for medicine is extraordinary. In the United States alone, over half-a-trillion dollars are spent each year to care for patients who suffer from tissue loss or dysfunction. Although numerous books and reviews have been written on tissue engineering, none has been as comprehensive in its defining of the field. Principles of Tissue Engineering combines in one volume the prerequisites for a general understanding of tissue growth and development, the tools and theoretical information needed to design tissues and organs, as well as a presentation of applications of tissue engineering to diseases affecting specific organ systems. The first edition of the book, published in 1997, is the definite reference in the field. Since that time, however, the discipline has grown tremendously, and few experts would have been able to predict the explosion in our knowledge of gene expression, cell growth and differentiation, the variety of stem cells, new polymers and materials that are now available, or even the successful introduction of the first tissue-engineered products into the marketplace. There was a need for a new edition, and this need has been met with a product that defines and captures the sense of excitement, understanding and anticipation that has followed from the evolution of this fascinating and important field. Key Features* Provides vast, detailed analysis of research on all of the major systems of the human body, e.g., skin, muscle, cardiovascular, hematopoietic, and nerves* Essential to anyone working in the field* Educates and directs both the novice and advanced researcher* Provides vast, detailed analysis of research with all of the major systems of the human body, e.g. skin, muscle, cardiovascular, hematopoietic, and nerves* Has new chapters written by leaders in the latest areas of research, such as fetal tissue engineering and the universal cell* Considered the definitive reference in the field* List of contributors reads like a "who's who" of tissue engineering, and includes Robert Langer, Joseph Vacanti, Charles Vacanti, Robert Nerem, A. Hari Reddi, Gail Naughton, George Whitesides, Doug Lauffenburger, and Eugene Bell, among others
