Countercurrent Gradient Chromatography (CGC) PDF Download
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Author: Lisa Evans Curry
Publisher:
ISBN:
Category :
Languages : en
Pages : 362
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Book Description
Author: Lisa Evans Curry
Publisher:
ISBN:
Category :
Languages : en
Pages : 362
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Book Description
Author: A. Berthod
Publisher: Elsevier
ISBN: 0080929664
Category : Science
Languages : en
Pages : 310
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Book Description
Countercurrent chromatography (CCC) is a separation technique in which the stationary phase is a liquid. The mobile phase is also a liquid, so biphasic liquid systems with at least two solvents are used. Centrifugal fields are used to hold the liquid stationary phase while pushing the liquid mobile phase through it.This comprehensive reference covers recent advancements in the two types of CCC machines: the high speed CCCs without rotary seals and with coiled spools and centrifugal partition chromatographs (CPC) with rotary seals and interconnected channels. Written by leading international experts in the CCC field, the book focuses on the liquid nature of the stationary phase: giving newcomers the basis to do CCC efficiently and rapidly; explaining the art of obtaining a biphasic liquid system; describing the flow patterns in both CPC and high speed CCC machines; showing possible other uses of a liquid stationary phase; presenting a wealth of applications in the separation of organic, pharmaceutical and inorganic mixtures; and demonstrating that even supercritical fluids can be used in CCC.
Author: Jean-Michel Menet
Publisher: CRC Press
ISBN: 9780824799922
Category : Science
Languages : en
Pages : 302
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Book Description
Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, a versatile technique for purification in biotechnology applications. The text investigates mechanisms for mixing liquid phases, particularly hydrostatic techniques for CPC and hydrodynamic for coil planet centrifuges. It also explores the use of countercurrent chromatography in inorganic analysis, chiral separation, and the separation of natural products.
Author: Walter D. Conway
Publisher: Wiley-VCH
ISBN:
Category : Science
Languages : en
Pages : 504
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 238
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Book Description
Author: Yoichiro Ito
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 488
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Book Description
High-Speed Countercurrent Chromatography is the first book to provide a comprehensive and up-to-date treatment of this technique. It covers all the latest developments in equipment, theory, and applications, as well as many topics not previously published anywhere, such as the purification of recombinant proteins directly from a crude E. coli lysate, the development of instruments that produce highly concentrated pure fractions, and successful CCC/MS interfacing.
Author: Lloyd R. Snyder
Publisher: John Wiley & Sons
ISBN: 0470055510
Category : Science
Languages : en
Pages : 496
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Book Description
Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.
Author: Jean-Michel Menet
Publisher: CRC Press
ISBN: 9780824799922
Category : Science
Languages : en
Pages : 298
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Book Description
Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, a versatile technique for purification in biotechnology applications. The text investigates mechanisms for mixing liquid phases, particularly hydrostatic techniques for CPC and hydrodynamic for coil planet centrifuges. It also explores the use of countercurrent chromatography in inorganic analysis, chiral separation, and the separation of natural products.
Author: Alain Berthod
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Walter D. Conway
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 256
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Book Description
Presents theoretical and practical aspects of the most recent advances in countercurrent chromatography, including pH-zone-refining countercurrent chromatography. Examines the use of countercurrent chromatography for the measurement of octanol/water partition coefficients. Describes separation of alkaloids and other pharmacologically active substances from plants. Discusses optimization of solvent systems and reviews the purification of peptides, lipoproteins, and commercial dyes. Includes information on multilayer coil and cross axis countercurrent chromatography and presents theoretical models for comparison and optimization.
