Author: Daniela Cecconi
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Comparative Proteomics Analysis
Author: Daniela Cecconi
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Bioinformatics for Comparative Proteomics
Author: Cathy H. Wu
Publisher: Humana Press
ISBN: 9781493957903
Category : Science
Languages : en
Pages : 387
Book Description
With the rapid development of proteomic technologies in the life sciences and in clinical applications, many bioinformatics methodologies, databases, and software tools have been developed to support comparative proteomics study. In Bioinformatics for Comparative Proteomics, experts in the field highlight the current status, challenges, open problems, and future trends for developing bioinformatics tools and resources for comparative proteomics research in order to deliver a definitive reference providing both the breadth and depth needed on the subject. Structured in three major sections, this detailed volume covers basic bioinformatics frameworks relating to comparative proteomics, bioinformatics databases and tools for proteomics data analysis, and integrated bioinformatics systems and approaches for studying comparative proteomics in the systems biology context. Written for the highly successful Methods in Molecular BiologyTM series, the contributions in this book provide the meticulous, step-by-step description and implementation advice that is crucial for getting optimal results in the lab. Comprehensive and easy-to-use, Bioinformatics for Comparative Proteomics serves all readers who wish to learn about state-of-the-art bioinformatics databases and tools, novel computational methods and future trends in proteomics data analysis, and comparative proteomics in systems biology.
Publisher: Humana Press
ISBN: 9781493957903
Category : Science
Languages : en
Pages : 387
Book Description
With the rapid development of proteomic technologies in the life sciences and in clinical applications, many bioinformatics methodologies, databases, and software tools have been developed to support comparative proteomics study. In Bioinformatics for Comparative Proteomics, experts in the field highlight the current status, challenges, open problems, and future trends for developing bioinformatics tools and resources for comparative proteomics research in order to deliver a definitive reference providing both the breadth and depth needed on the subject. Structured in three major sections, this detailed volume covers basic bioinformatics frameworks relating to comparative proteomics, bioinformatics databases and tools for proteomics data analysis, and integrated bioinformatics systems and approaches for studying comparative proteomics in the systems biology context. Written for the highly successful Methods in Molecular BiologyTM series, the contributions in this book provide the meticulous, step-by-step description and implementation advice that is crucial for getting optimal results in the lab. Comprehensive and easy-to-use, Bioinformatics for Comparative Proteomics serves all readers who wish to learn about state-of-the-art bioinformatics databases and tools, novel computational methods and future trends in proteomics data analysis, and comparative proteomics in systems biology.
Algorithms for Comparative Sequence Analysis and Comparative Proteomics
Author: Amol Prakash
Publisher:
ISBN:
Category : Genomics
Languages : en
Pages : 127
Book Description
Publisher:
ISBN:
Category : Genomics
Languages : en
Pages : 127
Book Description
Mass Spectrometry Applications for Comparative Proteomics and Peptidomic Discovery
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
In this thesis, multiple biological samples from various diseases models or treatments are investigated using shotgun proteomics and improved methods are developed to enable extended characterization and detection of neuropeptides. In general, this thesis aims to expand upon the rapidly evolving field of mass spectrometry (MS)-based proteomics and peptidomics by primarily enhancing small scale sample analysis. A review of the current status and progress in the field of biomarker discovery in peptidomics and proteomics is presented. To this rapidly expanding body of literature, our critical review offers new insights into MS-based biomarker studies investigating numerous biological samples, methods for post-translational modifications, quantitative proteomics, and biomarker validation. Methods are developed and presented, including immunodepletion for small volume cerebrospinal fluid (CSF) samples, for comparison of the CSF proteomes between an Alexander disease transgenic mouse model with overexpression of the glial fibrillary acidic protein and a control animal. This thesis also covers the application of the small scale immunodepletion of CSF for comparative proteomic analysis of a novel rat adapted scrapie (RAS) model for prion disease and compares the RAS CSF proteome to control rat CSF using MS. Large scale phosphoproteomics of starved vs. glucose fed yeast is presented to better understand the phosphoproteome changes that occur during glucose feeding. Method development for neuropeptide analysis is expanded upon using electron transfer dissociation (ETD) fragmentation to successfully sequence, for the first time, the crustacean hyperglycemic hormone precursor-related peptide (CPRP) from the blue crab Callinectes sapidus. In addition, a method for ETD sequencing of sulfonated neuropeptides using a magnesium salt adduct in an ion trap mass spectrometer is reported. This thesis also reports on a method for sub-ìg peptide isolation when using a molecular weight cut-off filtration device to improve sample recovery by over 2 orders of magnitude. All the protocols used throughout the work are provided in an easy to use step-by-step format in the Appendix. Collectively, this body of work extends the capabilities of mass spectrometry as a bioanalytical tool for shotgun proteomics and expands upon methods for neuropeptide discovery and analysis.
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
In this thesis, multiple biological samples from various diseases models or treatments are investigated using shotgun proteomics and improved methods are developed to enable extended characterization and detection of neuropeptides. In general, this thesis aims to expand upon the rapidly evolving field of mass spectrometry (MS)-based proteomics and peptidomics by primarily enhancing small scale sample analysis. A review of the current status and progress in the field of biomarker discovery in peptidomics and proteomics is presented. To this rapidly expanding body of literature, our critical review offers new insights into MS-based biomarker studies investigating numerous biological samples, methods for post-translational modifications, quantitative proteomics, and biomarker validation. Methods are developed and presented, including immunodepletion for small volume cerebrospinal fluid (CSF) samples, for comparison of the CSF proteomes between an Alexander disease transgenic mouse model with overexpression of the glial fibrillary acidic protein and a control animal. This thesis also covers the application of the small scale immunodepletion of CSF for comparative proteomic analysis of a novel rat adapted scrapie (RAS) model for prion disease and compares the RAS CSF proteome to control rat CSF using MS. Large scale phosphoproteomics of starved vs. glucose fed yeast is presented to better understand the phosphoproteome changes that occur during glucose feeding. Method development for neuropeptide analysis is expanded upon using electron transfer dissociation (ETD) fragmentation to successfully sequence, for the first time, the crustacean hyperglycemic hormone precursor-related peptide (CPRP) from the blue crab Callinectes sapidus. In addition, a method for ETD sequencing of sulfonated neuropeptides using a magnesium salt adduct in an ion trap mass spectrometer is reported. This thesis also reports on a method for sub-ìg peptide isolation when using a molecular weight cut-off filtration device to improve sample recovery by over 2 orders of magnitude. All the protocols used throughout the work are provided in an easy to use step-by-step format in the Appendix. Collectively, this body of work extends the capabilities of mass spectrometry as a bioanalytical tool for shotgun proteomics and expands upon methods for neuropeptide discovery and analysis.
Comparative Proteomics: An Approach to Elucidating the Function of a Novel Gene Called BRE.
Author: Kenneth Ka Ho Lee
Publisher:
ISBN: 9789533078328
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN: 9789533078328
Category :
Languages : en
Pages :
Book Description
Comparative Proteomic Analysis and Identification of Proteins Expressed by Peltaster Sp.1 in Jojoba Oil
Author: Jana Frank
Publisher:
ISBN:
Category :
Languages : en
Pages : 108
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 108
Book Description
Subcellular Proteomics
Author: Eric Bertrand
Publisher: Springer Science & Business Media
ISBN: 1402059434
Category : Science
Languages : en
Pages : 397
Book Description
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
Publisher: Springer Science & Business Media
ISBN: 1402059434
Category : Science
Languages : en
Pages : 397
Book Description
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
Proteomics
Author: Tsz Kwong Man
Publisher: IntechOpen
ISBN: 9789533078328
Category : Science
Languages : en
Pages : 452
Book Description
Biomedical research has entered a new era of characterizing a disease or a protein on a global scale. In the post-genomic era, Proteomics now plays an increasingly important role in dissecting molecular functions of proteins and discovering biomarkers in human diseases. Mass spectrometry, two-dimensional gel electrophoresis, and high-density antibody and protein arrays are some of the most commonly used methods in the Proteomics field. This book covers four important and diverse areas of current proteomic research: Proteomic Discovery of Disease Biomarkers, Proteomic Analysis of Protein Functions, Proteomic Approaches to Dissecting Disease Processes, and Organelles and Secretome Proteomics. We believe that clinicians, students and laboratory researchers who are interested in Proteomics and its applications in the biomedical field will find this book useful and enlightening. The use of proteomic methods in studying proteins in various human diseases has become an essential part of biomedical research.
Publisher: IntechOpen
ISBN: 9789533078328
Category : Science
Languages : en
Pages : 452
Book Description
Biomedical research has entered a new era of characterizing a disease or a protein on a global scale. In the post-genomic era, Proteomics now plays an increasingly important role in dissecting molecular functions of proteins and discovering biomarkers in human diseases. Mass spectrometry, two-dimensional gel electrophoresis, and high-density antibody and protein arrays are some of the most commonly used methods in the Proteomics field. This book covers four important and diverse areas of current proteomic research: Proteomic Discovery of Disease Biomarkers, Proteomic Analysis of Protein Functions, Proteomic Approaches to Dissecting Disease Processes, and Organelles and Secretome Proteomics. We believe that clinicians, students and laboratory researchers who are interested in Proteomics and its applications in the biomedical field will find this book useful and enlightening. The use of proteomic methods in studying proteins in various human diseases has become an essential part of biomedical research.
Comparative Proteomics of Tandem Mass Spectrometry Analyses for Bacterial Strains Identification and Differentiation
Author: Rabih E. Jabbour
Publisher:
ISBN: 9789535101413
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN: 9789535101413
Category :
Languages : en
Pages :
Book Description
Comparative Proteomics
Author: Thorsten Sven-Olaf Schmidt
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description