Chemical Biology Tools for Peptide and Protein Research PDF Download
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Author: Yu-Hsuan Tsai
Publisher: Frontiers Media SA
ISBN: 2889746682
Category : Science
Languages : en
Pages : 118
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Book Description
Author: Yu-Hsuan Tsai
Publisher: Frontiers Media SA
ISBN: 2889746682
Category : Science
Languages : en
Pages : 118
Get Book Here
Book Description
Author: Norbert Sewald
Publisher: John Wiley & Sons
ISBN: 3527802657
Category : Science
Languages : en
Pages : 771
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Book Description
Der "Sewald/Jakubke" ist und bleibt das einzige moderne, auf dem neuesten wissenschaftlichen Stand gehaltene fortgeschrittene Lehrbuch der Peptid-Biochemie! Auch für diese zweite, um ein Viertel erweitere Auflage haben die Autoren Hunderte von Publikationen gelesen und zu einem informativen und vollständigen Überblick über das Fachgebiet zusammengefasst. Nach einer Einführung in die Grundlagen der Peptidstruktur und -synthese werden die wichtigsten Familien biologisch aktiver Peptide systematisch abgehandelt. Mit interdisziplinärem Anspruch werden dann wichtige Anwendungen aus der Biotechnologie, Pharmazie und Biomedizin besprochen. Selbstverständlich werden auch Themen der Spitzenforschung berücksichtigt, zum Beispiel Pseudopeptide, Peptidmimetika und Aspekte der kombinatorischen Synthese. -- Neu in dieser Auflage sind Fragen und Übungsaufgaben, die das Nacharbeiten von Vorlesungen oder das Selbststudium erleichtern.
Author: Adam P. Arkin
Publisher: Wiley-Blackwell
ISBN: 9780470559260
Category :
Languages : en
Pages : 0
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Book Description
Author: Christopher Ahern
Publisher: Springer
ISBN: 1493928457
Category : Medical
Languages : en
Pages : 159
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Book Description
This volume describes chemical approaches to assess ion channel structure, function and pharmacology. Topics discussed include the use of engineered ionizable side chains to obtain information on permeation pathways and the local environment; the modification of engineered cysteine side chains, including cysteine scanning mutagenesis and the attachment of fluorescent probes and bio-reactive tethers; and the nascent use of genetic code expansion, evaluating its applications to ion channel and membrane proteins. This comprehensive text provides multifaceted perspectives on the great diversity of state-of-the-art methods which take advantage of the ever-expanding chemical toolbox to study ion channel biology. Capturing the contributions and innovations of renowned laboratory researchers in transmembrane protein study for the first time, this book is comprehensive in scope. It covers a wide array of experimental approaches: photochemistry, novel biological tools, and innovative spectroscopy, all combined with traditional techniques of electrophysiology and molecular biology. Novel Chemical Tools to Study Ion Channel Biology, part of the bestselling Advances in Experimental Medicine and Biology series is ideal for researchers and advanced students interested in biochemistry, biophysics, fluorometry, electrophysiology, and chemical biology. .
Author: John Howl
Publisher: Springer Science & Business Media
ISBN: 1592598773
Category : Science
Languages : en
Pages : 263
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Book Description
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Author: Craig Crews
Publisher: Royal Society of Chemistry
ISBN: 1788016866
Category : Medical
Languages : en
Pages : 382
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Book Description
Targeting protein degradation using small molecules is one of the most exciting small-molecule therapeutic strategies in decades and a rapidly growing area of research. In particular, the development of proteolysis targeting chimera (PROTACs) as potential drugs capable of recruiting target proteins to the cellular quality control machinery for elimination has opened new avenues to address traditionally 'difficult to target' proteins. This book provides a comprehensive overview from the leading academic and industrial experts on recent developments, scope and limitations in this dynamically growing research area; an ideal reference work for researchers in drug discovery and chemical biology as well as advanced students.
Author: Luca D. D'Andrea
Publisher: John Wiley & Sons
ISBN: 1119044138
Category : Science
Languages : en
Pages : 558
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Book Description
Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging. • Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab • Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source • Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics • Helps researchers choose the right chemical ligation technique for their needs
Author: Woojoo Eunice Kim
Publisher:
ISBN:
Category :
Languages : en
Pages : 173
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Book Description
Natural products, such as fatty acids, polyketides, and non-ribosomal peptides, exhibit diverse biological functions toward human health and are constructed via many different pathways; however, these pathways often share the same synthetic logic. Central to these pathways is a carrier protein (CP). The role of a CP is to transfer elongating intermediates between catalytic domains and is mediated by protein-protein interactions between the CP and its partner enzymes. These interactions are transient, making it difficult to understand how they communicate with each other in recognizing a cargo or how the CP meets the right partner enzymes. The Burkart laboratory has been developing fluorescent and mechanistic probes to understand these CP-partner protein interactions. The CP requires post-translational modification to become an active form through the action of 4'-phosphopantetheinyl transferase (PPTase) loading 4'phosphopantetheine prosthetic (PPant) arm at the serine residue. The first part of this dissertation focuses on utilizing the PPTase function to search for (1) the minimum peptide substrate required for CP recognition via machine learning and (2) the biosynthetic pathway from unculturable microorganisms. Using the ability of some PPTases to recognize and transfer an unnatural PPant arm to a CP, we identified orthogonal peptide substrates that can be labeled by two different classes of PPTases. These peptide substrates can be appended to a protein and used as a peptide tag. Furthermore, we employed a PPTase to fluorescently label CPs in previously uncultured microorganisms. This enabled us to sequence single cells for the identification of a biosynthetic cluster with active PPTase-CP pairs. In the second part of this dissertation, we developed two mechanistic probes to study protein-protein interactions between epimerization (E) domain and peptidyl carrier protein in non-ribosomal peptide synthetases. D-amino acids are incorporated into non-ribosomal peptides, which contribute to their unique conformation and bioactivity. The E domains convert L- to D-amino acids by deprotonating/reprotonating C[alpha]-H. Despite the past research on the E domain, the mechanistic details remain unclear. Herein, with the help of molecular dynamics simulations and mutational studies, our research reveals more detailed evidence on which catalytic residues work as an acid/base in this mechanism.
Author: Gregory Grant
Publisher: Oxford University Press
ISBN: 9780198030430
Category : Science
Languages : en
Pages : 404
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Book Description
The first synthetic peptides were produced a century ago. In the ensuing period, they have developed as valuable research tools that are readily available to all researchers. However, since most researchers do not make their own peptides, they are often unfamiliar with not only the synthetic chemistry but also with important and useful aspects of design, analysis, handling, and applications. This volume is the second edition of a volume that was first published 10 years ago. It is written by experts in the field who provide detailed descriptions as well as practical advice for producing and using synthetic peptides. The various chapters cover peptide design considerations, the synthetic chemistry, the evaluation of the synthetic product, and the modern applications of synthetic peptides. This includes the basic principles of peptide structure, analysis and chain assembly as well as the latest in selective disulfide bond formation, new strategies for the production of large peptides, and sequencing peptides by mass spectrometry. This book was designed with the intent of providing useful information both for the novices to the field as well as more seasoned practitioners. Its contents will help prevent problems commonly encountered and allow scientists to optimize their use of synthetic peptides.
Author: Hiroyuki Osada
Publisher: John Wiley & Sons
ISBN: 0470495006
Category : Science
Languages : en
Pages : 310
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Book Description
Discover the link between the latest chemical biology approaches and novel drug therapies! Protein Targeting with Small Molecules: Chemical Biology Techniques and Applications takes readers beyond the use of chemical biology in basic research, providing a highly relevant look at techniques that can address the challenges of biology and drug design and development. This indispensable bench companion features up-to-date coverage of advances in chemistry and assesses their impact on developing new therapeutics, making it ideal for chemical biologists and medicinal chemists who are developing small molecule drugs to target proteins and treat diseases. In addition, the book examines the full range of complex biological systems and their interrelationship with chemistry, from the interaction of biological response modifiers with proteins to the chemical biology of cell surface oligosaccharides. Distinguished by an overview of chemical biology that is reinforced and clarified by detailed examples and descriptions of techniques, Protein Targeting with Small Molecules: Chemical Biology Techniques and Applications: Introduces key technologies and methods of chemical biology designed to detect the interactions of small molecules and proteins Facilitates the discovery of small molecules that bind to proteins and describes the molecules' application in the investigation of biological processes Presents timely coverage of the development of fluorescent probes for small molecules, as well as the generation of small molecule ligands and inhibitors Reviews important techniques such as chemical genomics, target profiling, immobilization technology, detection methods, chemical inhibition, and structure-based targeting Offers a compelling synopsis of data that underscores the recent progress made in the area of targeting proteins by small molecules
