Characterization of the Escherichia Coli RecF and RecR Proteins and Their Role in Recombinational DNA Repair PDF Download
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Author: Brian Lynn Webb
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Author: Brian Lynn Webb
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Author: Andrei Kuzminov
Publisher: Landes Bioscience
ISBN:
Category : Medical
Languages : en
Pages : 234
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Author: Zachary Joseph Romero
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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In every round of replication, the replisome encounters various forms of damage such as DNA breaks, protein-DNA crosslinks, and oxidized bases. Escherichia coli has evolved multiple pathways to repair the various types of DNA damage that accumulates in the cell. Recombinational repair is a non-mutagenic repair pathway that utilizes a neighboring undamaged duplex to restore lost genetic information. Our lab has recently implicated the E. coli Uup and RadD in the recombinational repair of a wide variety of DNA lesions. We show that the SF2 helicase RadD is essential when another helicase, RecG, is removed from the cell. This synthetically lethal phenotype allowed us to leverage existing knowledge of RecG to better understand the functions that become essential in its absence. Functions that are specifically carried out by RadD. This synthetically lethal phenotype is rapidly suppressed by mutations that affect replication restart (PriA) and homologous recombination (RecA). We also identify that a full gene deletion of uup is capable of suppressing the [Delta]rad[Delta]recG phenotype. By using a variety of biochemical and genetic approaches we show that Uup and RadD are involved in the processing of the intermediates generated by RecA-dependent and independent recombination in every replication cycle. When this activity is lost, these intermediates are targeted for alternative processing, by PriA and Uup. This alternative processing has deleterious consequences and results in cell death. Altogether, the results presented here shed further light on Uup and RadD and their roles in recombinational repair.
Author: Linda Jean Roman
Publisher:
ISBN:
Category :
Languages : en
Pages : 694
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Author: Jac A. Nickoloff
Publisher: Springer Science & Business Media
ISBN: 1592590950
Category : Science
Languages : en
Pages : 417
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Book Description
Jac A. Nickoloff and Merl F. Hoekstra update and expand their two earlier acclaimed volumes (Vol. I: DNA Repair in Prokaryotes and Lower Eukaryotes and Vol. II: DNA Repair in Higher Eurkaryotes) with cutting-edge reviews by leading authorities of primary experimental findings about DNA repair processes in cancer biology. The reviews cover a wide range of topics from viruses and prokaryotes to higher eukaryotes, and include several new topics, among them the role of recombination in replication of damaged DNA, X-ray crystallographic analysis of DNA repair protein structures, DNA repair proteins and teleomere function, and the roles of BRCA1 and BRCA2 in DNA repair. Authoritative and up-to-date, DNA Damage and Repair, Vol. III: Advances from Phage to Humans surveys the rapidly moving research in DNA damage and repair, and explains the important functional relationships among different DNA repair pathways and the relationship between DNA repair pathways, cancer etiology, and cancer therapies.
Author: Andrés Aguilera
Publisher: Springer Science & Business Media
ISBN: 3540710213
Category : Science
Languages : en
Pages : 536
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Book Description
This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Author: Peter J. McInerney
Publisher:
ISBN:
Category :
Languages : en
Pages : 312
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Author: Nina Bonde
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Faithful replication of the genomic material is a requirement for successful cell propagation and division in all organisms. In Escherichia coli, the RadD and RecG proteins play important roles in DNA repair and recombination. This thesis reveals an important molecular function for RadD and examines RecG protein and gene interactions that help modulate and define its function in cells. We describe a new molecular function for RadD. RadD accelerates RecA-mediated strand exchange in vitro. The ability of RadD to accelerate strand exchange is dependent on both its ATP hydrolysis activity and the presence of RecA. RadD is unable to remodel recombination intermediate molecules in the absence of RecA. The dependence on RecA to carry out this function places RadD in a new class of enzymes. RecG physically interacts with the single-stranded DNA-binding protein (SSB). We report that this interaction is mediated by the highly conserved SSB C-terminal tip (SSB-Ct). The SSB-Ct docks onto a conserved region of the RecG Helicase Domain 2, and the SSB-Ct binding pocket on RecG is framed by three Arg residues. Charge-reversal mutations to any of the three Arg residues results in loss of the RecG-SSB interaction and cellular defects, including induced SOS response and sensitivity to ultraviolet radiation. As a complement to this study, we also report that the intrinsically disorder linker of SSB is not necessary under normal growth conditions and likely acts as a simple spacer between the SSB OB domain and SSB-Ct interaction motif. RecG function has been difficult to elucidate in vivo. We identify recG genetic interactions by transposon sequencing to gain insight into the activity of recG in cells. The strongest genetic interactions occur with the dam, uvrD, rnhA, radA, and rep genes. Viability of strains lacking recG in addition to one of these five genes is generally improved by additional loss of the recF or recO genes. recG mutants that are deficient in SSB binding produce similar phenotypes as cells lacking recG. Collectively, these results support a role for RecG in alleviating toxic recombination intermediates.
Author: Shiro Iuchi
Publisher: Springer Science & Business Media
ISBN: 0387274219
Category : Science
Languages : en
Pages : 291
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Book Description
In the early 1980s, a few scientists started working on a Xenopus transcription factor, TFIIIA. They soon discovered a novel domain associated with zinc, and named this domain "zinc finger. " Th e number of proteins with similar zinc fingers grew quickly and these proteins are now called C2H2, Cys2His2 or classical zinc finger proteins. To date, about 24,000 C2H2 zinc finger proteins have been recognized. Approximately 700 human genes, or more than 2% of the genome, have been estimated to encode C2H2 finger proteins. From the beginning these proteins were thought to be numerous, but no one could have predicted such a huge number. Perhaps thousands of scientists are now working on C2H2 zinc finger proteins fi-om variou s viewpoints. This field is a good example of how a new science begins with the insight of a few scientists and how it develops by efforts of numerous independent scientists, in contrast to a policy-driven scientific project, such as the Human Genome Project, with goals clearly set at its inception and with work performed by a huge collaboration throughout the world. As more zinc finger proteins were discovered, several subfamilies, such as C2C2, CCHC, CCCH, LIM, RING, TAZ, and FYVE emerged, increasing our understanding of zinc fingers. The knowledge was overwhelming. Moreover, scientists began defining the term "zinc finger" differently and using various names for identical zinc fingers. These complications may explain why no single comprehensive resource of zinc finger proteins was available before this publication.
Author: Inna Kruman
Publisher: BoD – Books on Demand
ISBN: 9533076976
Category : Medical
Languages : en
Pages : 652
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Book Description
The book consists of 31 chapters, divided into six parts. Each chapter is written by one or several experts in the corresponding area. The scope of the book varies from the DNA damage response and DNA repair mechanisms to evolutionary aspects of DNA repair, providing a snapshot of current understanding of the DNA repair processes. A collection of articles presented by active and laboratory-based investigators provides a clear understanding of the recent advances in the field of DNA repair.
