Author: D. Perrin
Publisher: Springer Science & Business Media
ISBN: 9400958749
Category : Science
Languages : en
Pages : 138
Get Book Here
Book Description
This book is intended as a practical manual for chemists, biologists and others whose work requires the use of pH or metal-ion buffers. Much information on buffers is scattered throughout the literature and it has been our endeavour to select data and instructions likely to be helpful in the choice of suitable buffer substances and for the preparation of appropriate solutions. For details of pH measurement and the preparation of standard acid and alkali solutions the reader is referred to a companion volume, A. Albert and E. P. Serjeant's The Determination of Ionization Constants (1971). Although the aims of the book are essentially practical, it also deals in some detail with those theoretical aspects considered most helpful to an understanding of buffer applications. We have cast our net widely to include pH buffers for particular purposes and for measurements in non-aqueous and mixed solvent systems. In recent years there has been a significant expansion in the range of available buffers, particularly for biological studies, largely in conse quence of the development of many zwiUerionic buffers by Good et al. (1966). These are described in Chapter 3.
Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0080521932
Category : Science
Languages : en
Pages : 811
Get Book Here
Book Description
Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes.Key Features* Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes* Over 1,500 definitions of kinetic and mechanistic terminology, with key references* Practical advice on experimental design of kinetic experiments* Extended step-by-step methods for deriving rate equations* Over 1,000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods* Over 5,000 selected references to kinetic methods appearing in the Methods in Enzymology series* 72-page Wordfinder that allows the reader to search by keywords* Summaries of mechanistic studies on key enzymes and protein systems* Over 250 diagrams, figures, tables, and structures
Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0123809258
Category : Science
Languages : en
Pages : 915
Get Book Here
Book Description
Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. - Historical analysis of kinetic principles including advanced enzyme science - Provides both theoretical and practical measurements tools - Coverage of single molecular kinetics - Examination of force generation mechanisms - Discussion of organic and inorganic enzyme reactions
Author: Murray P. Deutscher
Publisher: Gulf Professional Publishing
ISBN: 9780121820831
Category : Proteins
Languages : en
Pages : 936
Get Book Here
Book Description
Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Author: Hafiz Ahmed
Publisher: CRC Press
ISBN: 0203507436
Category : Medical
Languages : en
Pages : 410
Get Book Here
Book Description
Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different
Author:
Publisher:
ISBN:
Category : Mine safety
Languages : en
Pages : 850
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category : Abandoned mined lands reclamation
Languages : en
Pages : 436
Get Book Here
Book Description
Author: Professor Rob Beynon
Publisher: Taylor & Francis
ISBN: 1134994532
Category : Science
Languages : en
Pages : 97
Get Book Here
Book Description
An indispensable guide to buffers and to understanding the principles behind their use. Helps the user to avoid common errors in preparing buffers and their solutions. A must for researchers in the biological sciences, this valuable book takes the time to explain something often taken for granted - buffers used in experiments. It answers the common questions such as: which buffer should I choose? What about the temperature effects? What about ionic strength? Why is the buffer with the biggest temperature variation used in PCR? It provides even the most experienced researchers with the means to understand the fundamental principles behind their preparation and use - an indispensable guide essential for everyone using buffers.
Author: Arvind Kumar
Publisher: APH Publishing
ISBN: 9788176486910
Category : Environmental health
Languages : en
Pages : 372
Get Book Here
Book Description
57 Research Articles By Specialists In The Area That Highlight Hazards Like Biodegradation, Toxicological Affects, Heavy Metal Pollution, Effects Of Nitrogen And Farmyard Manners, Noise Pollution, Influence Of Soil Moisture, Weeds, Saline Soil Etc. Useful For Students, Teachers And Everyone Involved In The Subject.
Author: Daniel Purich
Publisher: CRC Press
ISBN: 1351730681
Category : Medical
Languages : en
Pages : 1368
Get Book Here
Book Description
Metabolic inhibitors and receptor antagonists are indispensable tools for the molecular life scientist. By blocking specific enzymes or receptor-mediated signal transduction cascades, they simplify the analysis of complex cellular processes especially when it is essential to demonstrate that a process of interest is functionally linked to a particular enzyme or receptor. From antibiotics to statins, modern medicine relies on the reliability and ease-of-use of enzyme- and receptor-directed inhibitors and antagonists.The Inhibitor Index is a comprehensive, curated compendium of over 7,800 enzyme inhibitors and receptor antagonists, including many toxins, poisons, and metabolic uncouplers.
