Biolistic DNA Delivery in Plants PDF Download
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Author: Sachin Rustgi
Publisher: Humana
ISBN: 9781071603581
Category : Science
Languages : en
Pages : 334
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Book Description
This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.
Author: Sachin Rustgi
Publisher: Humana
ISBN: 9781071603581
Category : Science
Languages : en
Pages : 334
Get Book Here
Book Description
This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.
Author: Leandro Peña
Publisher: Springer Science & Business Media
ISBN: 1592598277
Category : Science
Languages : en
Pages : 427
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Book Description
The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.
Author: John Flex Jackson
Publisher: Springer Science & Business Media
ISBN: 9783540002925
Category : Medical
Languages : en
Pages : 238
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Book Description
Whilst genetic transformation of plants is commonly viewed as a means of bringing about plant improvement, it has not so readily been recognised as a tool for analysing the function of plant genes. This book is unusual in that it focuses on the genetic transformation of a range of plants using a number of different methods. Many plants have been found to be quite difficult to transform, and so various techniques were developed. These techniques include: Agrobacterium suspension drops, electroporation, PEG, "whiskers", and various biolistic methods. A chapter on intellectual and property rights is included.
Author: Donald P. Weeks
Publisher: Academic Press
ISBN: 9780128117439
Category : Science
Languages : en
Pages : 300
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Book Description
Gene Editing in Plants, Volume 149, aims to provide the reader with an up-to-date survey of cutting edge research with gene editing tools and an overview of the implications of this research for progress in improving plant productivity and the nutritional quality of fruits, vegetables and grains. Leading scientists and researchers in the field of gene editing in plants describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing
Author: Saurabh Bhatia
Publisher: Academic Press
ISBN: 0128024984
Category : Medical
Languages : en
Pages : 452
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Book Description
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions
Author: Ingo Potrykus
Publisher: Springer Science & Business Media
ISBN: 3642792472
Category : Science
Languages : en
Pages : 370
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Book Description
Author: Ambikanandan Misra
Publisher: Elsevier
ISBN: 0123849659
Category : Science
Languages : en
Pages : 686
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Book Description
Delivery of therapeutic proteomics and genomics represent an important area of drug delivery research. Genomics and proteomics approaches could be used to direct drug development processes by unearthing pathways involved in disease pathogenesis where intervention may be most successful. This book describes the basics of genomics and proteomics and highlights the various chemical, physical and biological approaches to protein and gene delivery. - Covers a diverse array of topics from basic sciences to therapeutic applications of proteomics and genomics delivery - Of interest to researchers in both academia and industry - Highlights what's currently known and where further research is needed
Author: Hau-Hsuan Hwang
Publisher: Frontiers Media SA
ISBN: 2889195740
Category : Botany
Languages : en
Pages : 167
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Book Description
The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.
Author: Rosa León
Publisher: Springer Science & Business Media
ISBN: 0387755322
Category : Science
Languages : en
Pages : 145
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Book Description
Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.
Author: Matt Carter
Publisher: Academic Press
ISBN: 0323915612
Category : Medical
Languages : en
Pages : 416
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Book Description
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step
