Analytical Techniques in Immunochemistry PDF Download
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Author: Terry M. Phillips
Publisher: CRC Press
ISBN: 9780824784775
Category : Science
Languages : en
Pages : 370
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Book Description
Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of
Author: Terry M. Phillips
Publisher: CRC Press
ISBN: 9780824784775
Category : Science
Languages : en
Pages : 370
Get Book Here
Book Description
Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of
Author: Shirley J. Gee
Publisher: Cambridge University Press
ISBN: 0080946348
Category : Science
Languages : en
Pages : 121
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Book Description
This book is a tutorial designed to instruct the reader in use and application of immunochemical methods of analysis for environmental contaminants. A brief introduction describes basic principles and the advantages and disadvantages of the technology, and gives a listing of references which supply more detail. Preparation of the laboratory for use of this technology and the general scientific considerations prior to using the technology are discussed. Detailed step-wise procedures are given for analysis of selected analytes, triazine herbicides, carbaryl, paraquat, and p-nitrophenols, etc. In addition to the specific immunoassay methods, a series of support techniques necessary to perform immunochemical methods are described. This book provides specific instruction for certain analytes, but also serves to familiarize the novice reader with many generic concepts needed to successfully utilize immunochemistry technology including: applications, sampling, sample preparation, extraction, cleanup, quality assurance, methods development and optimization, data handling and troubleshooting. It is not necessary for the reader to actually perform the immunoassays given in this user's guide to obtain familiarity with these concepts. The guide is written so that the information presented can be applied to other immunoassays not given here. Thus, the strength of the guide is its universal applicability to immunoassay methods.
Author: Chukwuebuka Egbuna
Publisher: Academic Press
ISBN: 0128227990
Category : Science
Languages : en
Pages : 302
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Book Description
Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry.The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. - Presents basic analytical protocols and sample-preparation guidelines - Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry - Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research - Presents biostatistical tools and methods and basic computational models in biosciences
Author: Igor B. Buchwalow
Publisher: Springer Science & Business Media
ISBN: 3642046096
Category : Medical
Languages : en
Pages : 153
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Book Description
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Author: Miroslav Ferencik
Publisher: Springer Science & Business Media
ISBN: 9401115524
Category : Science
Languages : en
Pages : 529
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Book Description
The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.
Author: John E. Butler
Publisher: CRC Press
ISBN: 9780849353949
Category : Science
Languages : en
Pages : 334
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Book Description
Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.
Author: Shan-Rong Shi
Publisher: Eaton Publishing Company/Biotechniques Books
ISBN:
Category : Medical
Languages : en
Pages : 388
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Book Description
Author: A. Nowotny
Publisher: Springer Science & Business Media
ISBN: 3642673562
Category : Medical
Languages : en
Pages : 328
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Book Description
Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 0521516358
Category : Medical
Languages : en
Pages : 769
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Book Description
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author: Fan Lin
Publisher: Springer
ISBN: 1493915789
Category : Medical
Languages : en
Pages : 756
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Book Description
In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
