An in Vitro Comparison of the Osteogenic Potential of Equine Stem Cell Populations and Subpopulations from Multiple Tissue Sources PDF Download
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Author: Catherine L. Radtke
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
An in vitro comparison of the osteogenic potential of equine stem cell populations and subpopulations from multiple tissue sources was made to identify the ideal equine donor tissue as a source of MSCs to promote bone healing. Equine muscle tissue- and periosteal tissue-derived cells where characterized as mesenchymal stem cells (MSCs) and their proliferation capacity and osteogenic potential was assessed in comparison with bone marrow- and adipose tissue-derived MSCs. Cells were isolated from skeletal muscle, periosteal, and adipose tissues, and sternal bone marrow aspirates. Morphology, adherence to plastic, trilineage differentiation, and detection of stem cell surface markers CD44 and CD90 were used to characterize cells as MSCs. Osteogenic potential of MSCs was measured by osteocalcin gene expression. Mesenchymal stromal cell cultures were counted at 24, 48, 72, and 96 hours to determine tissue-specific MSC proliferative capacity. Muscle MSCs (MMSCs), periosteum MSCs (PMSCs), and adipose MSCs (AMSCs) proliferated significantly faster than did bone marrow MSCs (BMSCs) at 72 and 96 hours. Non-equilibrium gravitational field-flow fractionation (GrFFF) was validated as a method for sorting MSCs from four donor sources (muscle, periosteum, bone marrow, and adipose tissue) into subpopulations. Aliquots of MSCs from each tissue source were consistently separated into 6 fractions by continuous flow (GrFFF proprietary system) and these fractions remained viable for use in further assays. Absorbencies (OD) were compared, and trilineage assays performed. Statistical analysis of the fraction absorbencies (OD) revealed a P-value of
Author: Catherine L. Radtke
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
An in vitro comparison of the osteogenic potential of equine stem cell populations and subpopulations from multiple tissue sources was made to identify the ideal equine donor tissue as a source of MSCs to promote bone healing. Equine muscle tissue- and periosteal tissue-derived cells where characterized as mesenchymal stem cells (MSCs) and their proliferation capacity and osteogenic potential was assessed in comparison with bone marrow- and adipose tissue-derived MSCs. Cells were isolated from skeletal muscle, periosteal, and adipose tissues, and sternal bone marrow aspirates. Morphology, adherence to plastic, trilineage differentiation, and detection of stem cell surface markers CD44 and CD90 were used to characterize cells as MSCs. Osteogenic potential of MSCs was measured by osteocalcin gene expression. Mesenchymal stromal cell cultures were counted at 24, 48, 72, and 96 hours to determine tissue-specific MSC proliferative capacity. Muscle MSCs (MMSCs), periosteum MSCs (PMSCs), and adipose MSCs (AMSCs) proliferated significantly faster than did bone marrow MSCs (BMSCs) at 72 and 96 hours. Non-equilibrium gravitational field-flow fractionation (GrFFF) was validated as a method for sorting MSCs from four donor sources (muscle, periosteum, bone marrow, and adipose tissue) into subpopulations. Aliquots of MSCs from each tissue source were consistently separated into 6 fractions by continuous flow (GrFFF proprietary system) and these fractions remained viable for use in further assays. Absorbencies (OD) were compared, and trilineage assays performed. Statistical analysis of the fraction absorbencies (OD) revealed a P-value of
Author: Jeffrey M. Gimble
Publisher: Humana Press
ISBN: 9781617379611
Category : Science
Languages : en
Pages : 474
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Book Description
During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.
Author: Lucas G. Chase
Publisher: Springer Science & Business Media
ISBN: 1627032002
Category : Science
Languages : en
Pages : 458
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Book Description
Over the past decade, significant efforts have been made to develop stem cell-based therapies for difficult to treat diseases. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), appear to hold great promise in regards to a regenerative cell-based therapy for the treatment of these diseases. Currently, more than 200 clinical trials are underway worldwide exploring the use of MSCs for the treatment of a wide range of disorders including bone, cartilage and tendon damage, myocardial infarction, graft-versus-host disease, Crohn’s disease, diabetes, multiple sclerosis, critical limb ischemia and many others. MSCs were first identified by Friendenstein and colleagues as an adherent stromal cell population within the bone marrow with the ability to form clonogenic colonies in vitro. In regards to the basic biology associated with MSCs, there has been tremendous progress towards understanding this cell population’s phenotype and function from a range of tissue sources. Despite enormous progress and an overall increased understanding of MSCs at the molecular and cellular level, several critical questions remain to be answered in regards to the use of these cells in therapeutic applications. Clinically, both autologous and allogenic approaches for the transplantation of MSCs are being explored. Several of the processing steps needed for the clinical application of MSCs, including isolation from various tissues, scalable in vitro expansion, cell banking, dose preparation, quality control parameters, delivery methods and numerous others are being extensively studied. Despite a significant number of ongoing clinical trials, none of the current therapeutic approaches have, at this point, become a standard of care treatment. Although exceptionally promising, the clinical translation of MSC-based therapies is still a work in progress. The extensive number of ongoing clinical trials is expected to provide a clearer path forward for the realization and implementation of MSCs in regenerative medicine. Towards this end, reviews of current clinical trial results and discussions of relevant topics association with the clinical application of MSCs are compiled in this book from some of the leading researchers in this exciting and rapidly advancing field. Although not absolutely all-inclusive, we hope the chapters within this book can promote and enable a better understanding of the translation of MSCs from bench-to-bedside and inspire researchers to further explore this promising and quickly evolving field.
Author: Phuc Van Pham
Publisher: Springer
ISBN: 3319401440
Category : Science
Languages : en
Pages : 316
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Book Description
This invaluable resource discusses clinical applications with effects and side-effects of applications of stem cells in bone and cartilage regeneration. Each chapter is contributed by a pre-eminent scientist in the field and covers such topics as skeletal regeneration by mesenchymal stem cells, clinical improvement of mesenchymal stem cell injection in injured cartilage and osteoarthritis, Good manufacturing practice (GMP), minimal critera of stem cells for clinical applications, future directions of the discussed therapies and much more. Bone & Cartilage Regeneration and the other books in the Stem Cells in Clinical Applications series will be invaluable to scientists, researchers, advanced students and clinicians working in stem cells, regenerative medicine or tissue engineering.
Author: Ariff Bongso
Publisher: World Scientific
ISBN: 9814289388
Category : Science
Languages : en
Pages : 721
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Book Description
Stem cell biology has drawn tremendous interest in recent years as it promises cures for a variety of incurable diseases. This book deals with the basic and clinical aspects of stem cell research and involves work on the full spectrum of stem cells isolated today. It also covers the conversion of stem cell types into a variety of useful tissues which may be used in the future for transplantation therapy. It is thus aimed at undergraduates, postgraduates, scientists, embryologists, doctors, tissue engineers and anyone who wishes to gain some insight into stem cell biology. This book is important as it is comprehensive and covers all aspects of stem cell biology, from basic research to clinical applications. It will have 33 chapters written by renowned stem cell scientists worldwide. It will be up-to-date and all the chapters include self-explanatory figures, color photographs, graphics and tables. It will be easy to read and give the reader a complete understanding and state of the art of the exciting science and its applications.
Author: Melvin A. Shiffman
Publisher: Springer
ISBN: 3642452078
Category : Medical
Languages : en
Pages : 815
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Book Description
Interest in the use of stem cells in aesthetic procedures has been increasing rapidly, reflecting the widespread acknowledgment of the tremendous potential of stem cell fat transfer. This is, however, the first book to be devoted entirely to the subject. The book opens by reviewing the history of the development of pluripotent stem cells and the results of research into the biochemistry and physiology of stem cells. Adipose tissue anatomy and survival are discussed and the wide range of aesthetic procedures involving stem cell fat transfer are then described in detail. These procedures relate to the face, breast, buttocks, legs, hands, penis and Poland syndrome. In addition, potential risks and complications are identified. The book has been written by leading experts and will be an invaluable source of information for students, beginners and experienced surgeons in a range of specialties.
Author: George J. Christ
Publisher: Cambridge University Press
ISBN: 0521899494
Category : Medical
Languages : en
Pages : 361
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Book Description
A state-of-the-art primer on the role of pharmacological sciences in regenerative medicine, for advanced students, postdoctoral fellows, and researchers.
Author: Phuc Van Pham
Publisher: BoD – Books on Demand
ISBN: 9535136151
Category : Science
Languages : en
Pages : 302
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Book Description
Mesenchymal Stem Cells: Isolation, Characterization, and Applications thoroughly presents the isolation, characterization, and some applications of mesenchymal stem cells in the clinic. The book has two parts: "Isolation and Characterization" and "Clinical Perspectives and Applications." In Part I, the subsequent chapters introduce some techniques in isolation, characterization, and purification of mesenchymal stem cells in different tissues. In Part II, some applications of mesenchymal stem cells in the popular diseases, which include cartilage regeneration, spinal cord injury, and osteoarthritis, are discussed. This book provides a succinct yet comprehensive overview of mesenchymal stem cells for advanced students, graduate students, and researchers.
Author: Janette K. Burgess
Publisher: Springer Nature
ISBN: 303029403X
Category : Science
Languages : en
Pages : 260
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Book Description
This book presents state-of-the-art pre-clinical models and clinical applications of stem-cell-based therapies applied to different lung diseases, with a special focus on the translation of bench data into clinical studies. Starting with the assumption that abnormal lung tissue repair and regeneration has emerged as the driving force underlying pathogenesis and progression in many lung diseases, it sheds new light on the potential of stem/stromal cells as drivers of repair and sources of reparative factors in the lung. The first part of the book offers an overview of stem cell types and mechanisms involved in lung development, homeostasis, repair and regeneration, and reveals the crucial role of the extracellular matrix within the lung microenvironment. In the second part, leading experts present the latest pre-clinical evidence and clinical applications of stem-cell-based therapies in a wide variety of lung diseases, ranging from COPD and lung fibrosis to other rare lung diseases. The last section discusses stem cell delivery systems and devices, such as aerosolised spray application. This book appeals to pneumologists, stem cell and matrix biologists, as well as bioengeneers with a special interest in regenerative medicine applied to pulmonary diseases.
Author: Anthony Atala
Publisher: Academic Press
ISBN: 0124104576
Category : Medical
Languages : en
Pages : 607
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Book Description
Translational Regenerative Medicine is a reference book that outlines the life cycle for effective implementation of discoveries in the dynamic field of regenerative medicine. By addressing science, technology, development, regulatory, manufacturing, intellectual property, investment, financial, and clinical aspects of the field, this work takes a holistic look at the translation of science and disseminates knowledge for practical use of regenerative medicine tools, therapeutics, and diagnostics. Incorporating contributions from leaders in the fields of translational science across academia, industry, and government, this book establishes a more fluid transition for rapid translation of research to enhance human health and well-being. Provides formulaic coverage of the landscape, process development, manufacturing, challenges, evaluation, and regulatory aspects of the most promising regenerative medicine clinical applications Covers clinical aspects of regenerative medicine related to skin, cartilage, tendons, ligaments, joints, bone, fat, muscle, vascular system, hematopoietic /immune system, peripheral nerve, central nervous system, endocrine system, ophthalmic system, auditory system, oral system, respiratory system, cardiac system, renal system, hepatic system, gastrointestinal system, genitourinary system Identifies effective, proven tools and metrics to identify and pursue clinical and commercial regenerative medicine
