Affinity Labeling PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Affinity Labeling PDF full book. Access full book title Affinity Labeling by William B. Jakoby. Download full books in PDF and EPUB format.
Author: William B. Jakoby
Publisher: Academic Press
ISBN:
Category : Science
Languages : en
Pages : 808
Get Book Here
Book Description
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Author: William B. Jakoby
Publisher: Academic Press
ISBN:
Category : Science
Languages : en
Pages : 808
Get Book Here
Book Description
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Author: Dmitri G Knorre
Publisher: CRC Press
ISBN: 1351078011
Category : Medical
Languages : en
Pages : 277
Get Book Here
Book Description
The goal of this book is to give a systematic description of the main principles of affinity modification and applications, consideration of possibilities, and restrictions of the method. Modification within specific complexes is a special case of chemical modification which is widely used in the nonaddressed version in biochemistry and related areas. Therefore, we have included in the first introductory paper chapter of the book general considerations of chemical modifications of biopolymers and the application of biopolymers.
Author: Murat Sunbul
Publisher: Springer Nature
ISBN: 1493995375
Category : Science
Languages : en
Pages : 231
Get Book Here
Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Alfred Nisonoff
Publisher: Academic Press
ISBN: 1483273857
Category : Nature
Languages : en
Pages : 560
Get Book Here
Book Description
The Antibody Molecule reviews the literature leading to current knowledge of the structure of immunoglobulins. The book begins by outlining some of the basic structural characteristics of immunoglobulins without citing the references on which the information is based. Separate chapters follow covering the chemical nature of the active site of an antibody molecule and mechanisms of interaction with hapten; the general structural features and properties of the various classes of human immunoglobulin; and amino acid sequences of human and mouse L chains and of human and rabbit H chains. Subsequent chapters deal with the evolution of the immunoglobulin classes; special properties of mouse, guinea pig, rabbit, and horse immunoglobulins; idiotypic specificities of immunglobulins; and the genetic control of antibodies. This book is meant for immunologists who have not personally observed the development of this exciting period in the history of immunology. It will also provide useful supplemental reading for the serious student or investigator who wishes to become familiar with the nature of the antibody molecule, its genetic control, and mode of action.
![Affinity Labeling of the Active Sites of [delta]5-ketosteroid Isomerase Using Photoexcited Natural Ligands PDF](https://books.google.com/books/content/images/frontcover/syKr_xTBvW0C?fife=w80-h100)
Author: Richard James Martyr
Publisher:
ISBN:
Category :
Languages : en
Pages : 172
Get Book Here
Book Description
Author: Murat Sunbul
Publisher: Humana
ISBN: 9781493995394
Category : Science
Languages : en
Pages : 231
Get Book Here
Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233
Get Book Here
Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author: Library of Congress
Publisher:
ISBN:
Category : Subject headings, Library of Congress
Languages : en
Pages : 1656
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 840
Get Book Here
Book Description
Author: Andrew D. Badley
Publisher: CRC Press
ISBN: 1040168299
Category : Medical
Languages : en
Pages : 556
Get Book Here
Book Description
In an effort to go beyond immune-based therapies, researchers are now considering the implications of apoptosis dysregulation during HIV-induced immunodeficiency. This work provides the first comprehensive compendium of the progress made in understanding the process of cell death related to HIV and the potential breakthroughs in treatment that offer much promise. Combining the work of more than two-dozen top researchers, this seminal volume provides clinicians and researchers with an excellent reference, while also serving as an incubator to stimulate future research. It explains the fundamental biology involved with apoptosis, explains its clinical impact in HIV, and examines the newest therapeutic approaches.
