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Author: Jan Breborowicz
Publisher: CRC Press
ISBN: 1351078003
Category : Science
Languages : en
Pages : 293
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Book Description
This book discusses the topic of affinity electrophoresis (AFF-EP), which has become a useful tool for studies of biomolecular interactions. The book will discuss AFF-EP as an analytical method which has been used successfully for the diagnosis, differentiation, and monitoring of patients with various diseases. The book will also discuss other uses for the AFF-EP method.
Author: Jan Breborowicz
Publisher: CRC Press
ISBN: 1351078003
Category : Science
Languages : en
Pages : 293
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Book Description
This book discusses the topic of affinity electrophoresis (AFF-EP), which has become a useful tool for studies of biomolecular interactions. The book will discuss AFF-EP as an analytical method which has been used successfully for the diagnosis, differentiation, and monitoring of patients with various diseases. The book will also discuss other uses for the AFF-EP method.
Author: Andras Guttman
Publisher: Newnes
ISBN: 0080931359
Category : Science
Languages : en
Pages : 391
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Book Description
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author: P. Mohr
Publisher: CRC Press
ISBN: 9780824774684
Category : Science
Languages : en
Pages : 320
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Book Description
Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.
Author: That T. Ngo
Publisher: Springer Science & Business Media
ISBN: 1489918728
Category : Science
Languages : en
Pages : 560
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Book Description
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Author: Budin Michov
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 3110761645
Category : Science
Languages : en
Pages : 521
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Book Description
Electrophoresis methods are simple and informative analytical methods, which are used in biochemistry and medicine. They are necessary for precise investigation of proteins and nucleic acids (DNA and RNA). Electrophoresis is practiced in chemical, biochemical, and clinical laboratories, as well as in forensic medicine laboratories. This book will discuss the fundamentals of electrophoresis methods at the graduate level.
Author: David S. Hage
Publisher: CRC Press
ISBN: 0824751981
Category : Science
Languages : en
Pages : 878
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Book Description
This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Author: Z. Deyl
Publisher: Elsevier
ISBN: 0080858198
Category : Science
Languages : en
Pages : 477
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Book Description
Applications
Author: Per Lundahl
Publisher: Routledge
ISBN: 1317836545
Category : Science
Languages : en
Pages : 244
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Book Description
First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.
Author: W. Ens
Publisher: Springer Science & Business Media
ISBN: 940159046X
Category : Science
Languages : en
Pages : 350
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Book Description
A NATO Advanced Research Workshop entitled New Methods for the Study of Molecular Aggregates was held at Tbe Lodge at Kananaskis Village, Alberta, Canada from 16 -20 June 1996. In fact the meeting was entirely concerned with the problem of analyzing biomolecular complexes, so the title of these proceedings has been altered to give a more precise description of the content. Tbe workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 participants from around the world. '!\venty-one invited talks were given and 27 papers were presented as posters. Of the 48 contributions, 22 papers (12 orals, 10 posters) are included in these proceedings. Tbe subject of the conference was the investigation of noncovalent biomolecular complexes, with particular focus on the application of mass spectrometry to their characterization. '!\vo new ionization techniques introduced in the late 1980s, electrospray ionization (ES I) and matrix-assisted laser desorptionlionization (MALDI), resulted in a breakthrough in mass spectrometry, enabling its use in molecular weight and primary structure determination of biopolymers larger than 100 kDa. Recently it has been discovered that ESI mass spectrometry mayaiso be used to characterize complexes containing noncovalent interactions, thus opening new perspectives for supramolecular chemistry. ESI mass spectrometry has the advantage that the sampie is introduced from a homogenous solution which can be maintained at near physiological conditions of pR, concentration, and temperature.
Author: H. Peeters
Publisher: Elsevier
ISBN: 1483279669
Category : Science
Languages : en
Pages : 1239
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Book Description
An International Review Series Devoted to Proteins and Related Studies, Volume 32: Protides of the Biological Fluids documents the proceedings of the 32nd Colloquium held in 1984. This compilation is organized into three parts. Part I begins with an introduction to the defense mechanism of immunoglobulins, followed by a description of enzymes and proteins, such as lactoperoxidase and lactoferrin. The secretions that include CSF, colostrum, milk, female and male genital secretions, saliva, gastrointestinal juice, nasal and bronchoalveolar secretions, tears and synovial fluid are also elaborated. The tumor markers, which include ferritin, CEA, and TPA are dealt with in Part II. The immunotoxins, tumor imaging, and lymphoid malignancies are also covered. Part III is devoted to the advances in the separation of protides, including the staining procedure for protein detection, two-dimensional electrophoresis, and HPLC and affinity chromatography. This book is valuable to biology students and clinicians of disciplines related to advances in the protein field.
