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Author: J. Turková
Publisher: Elsevier
ISBN: 9780080858128
Category : Science
Languages : en
Pages : 404
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Book Description
Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Author: J. Turková
Publisher: Elsevier
ISBN: 9780080858128
Category : Science
Languages : en
Pages : 404
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Book Description
Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Author: Ziad El Rassi
Publisher: Elsevier
ISBN: 0128245123
Category : Science
Languages : en
Pages : 956
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Book Description
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. Covers the basic principles of modern liquid phase separation techniques, along with their applications Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Author: P. Mohr
Publisher: CRC Press
ISBN: 9780824774684
Category : Science
Languages : en
Pages : 320
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Book Description
Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.
Author: Pascal Bailon
Publisher: Springer Science & Business Media
ISBN: 1603272615
Category : Science
Languages : en
Pages : 233
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Book Description
Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Author: David S. Hage
Publisher: CRC Press
ISBN: 0824751981
Category : Science
Languages : en
Pages : 878
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Book Description
This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Author: Peter D. G. Dean
Publisher: Oxford University Press, USA
ISBN:
Category : Science
Languages : en
Pages : 238
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Book Description
Matrix preparation and applications; Activation procedures; Cross-linking agents for coupling matrices to spacers; Operational methodologies; Ligands for immobilisation; Quantitative characterisation of interactions by affinity chromatography; Zonal elution quantitative affinity chromatography and analysis of molecular interactions; Cell separation by affinity chromatography ligand immobilisation to solid supports through cleavable mercury-sulphur bonds.
Author: Herbert Schott
Publisher: CRC Press
ISBN: 100014643X
Category : Science
Languages : en
Pages : 0
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Book Description
This book informs the reader about the practical methods, possibilities, and limits of template chromatography. It shows the various techniques for immobilization of nucleic acids fragments, polynucleotides, and nucleic acids by which the desired separation of materials can be achieved.
Author: A Abbott
Publisher: Elsevier
ISBN: 1845695089
Category : Technology & Engineering
Languages : en
Pages : 243
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Book Description
Biomimetic materials are those inspired from nature and implemented into new fibre and fabric technologies. Biologically inspired textiles explores the current state of the art in this research arena and examines how biomimetics are increasingly applied to new textile technologies. Part one discusses the principles, production and properties of biomimetics. Chapters include recombinant DNA technologies and their application for protein production, spinning of fibres from protein solutions and structure/function relationships in spider silk. The second part of the book provides a review of the application of biomimetics to a range of textile applications, including the design of clothing and self cleaning textiles. Written by a distinguished team of international authors, Biologically inspired textiles is a valuable reference for textile technologists, fibre scientists, textile manufacturers and others in academia. Discusses the principles, production and properties of biomimetics Reviews the application of biomimetics to a range of textile disciplines Chapters explore recombinant DNA technologies, spinning of fibres and structure/function relationships in spider silk
Author: That T. Ngo
Publisher: Springer Science & Business Media
ISBN: 1489918728
Category : Science
Languages : en
Pages : 560
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Book Description
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Author: Jan-Christer Janson
Publisher: John Wiley & Sons
ISBN: 9780471186267
Category : Medical
Languages : en
Pages : 712
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Book Description
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
