Advanced Micropipette Techniques for Cell Physiology PDF Download
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Author: Kenneth T. Brown
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 320
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Book Description
IBRO Handbook Series: Methods in the Neurosciences, Volume 9 General Editor: A. D. Smith Advanced Micropipette Techniques for Cell Physiology Kenneth T. Brown and Dale G. Flaming With this Handbook Series, IBRO aims to fill a major gap in the world literature. The neuroscientist needs to be able to turn to whichever specialized method is most suited to the problem he is currently studying. Methods in the Neurosciences will help to provide expert advice on exactly how to carry out the experiments, on what difficulties can occur and on the limitations of the method. Fine glass micropipettes are extensively used in intra and extra-cellular physiology as a means of recording electrical activity in cells and as a way of injecting a variety of substances for experimental purposes. For the last 12 years the authors of this book have treated micropipette techniques as a research field in itself, and here present for the first time their theory of how micropipette tips are formed, their methods of reducing tip size, and the implications of their work for research on small cells of all kinds, especially cells within the central nervous system. The book not only incorporates this new work but reviews and analyses existing publications on micropipette methodology, including patch-clamping, in order to present as complete an account as possible of how micropipettes can be used efficiently and effectively in a wide variety of experimental situations.
Author: Kenneth T. Brown
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 320
Get Book Here
Book Description
IBRO Handbook Series: Methods in the Neurosciences, Volume 9 General Editor: A. D. Smith Advanced Micropipette Techniques for Cell Physiology Kenneth T. Brown and Dale G. Flaming With this Handbook Series, IBRO aims to fill a major gap in the world literature. The neuroscientist needs to be able to turn to whichever specialized method is most suited to the problem he is currently studying. Methods in the Neurosciences will help to provide expert advice on exactly how to carry out the experiments, on what difficulties can occur and on the limitations of the method. Fine glass micropipettes are extensively used in intra and extra-cellular physiology as a means of recording electrical activity in cells and as a way of injecting a variety of substances for experimental purposes. For the last 12 years the authors of this book have treated micropipette techniques as a research field in itself, and here present for the first time their theory of how micropipette tips are formed, their methods of reducing tip size, and the implications of their work for research on small cells of all kinds, especially cells within the central nervous system. The book not only incorporates this new work but reviews and analyses existing publications on micropipette methodology, including patch-clamping, in order to present as complete an account as possible of how micropipettes can be used efficiently and effectively in a wide variety of experimental situations.
Author: Rosemary Martin
Publisher: CRC Press
ISBN: 1000725359
Category : Science
Languages : en
Pages : 280
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Book Description
Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.
Author: Ellen Covey
Publisher: Oxford University Press, USA
ISBN: 0199939802
Category : Medical
Languages : en
Pages : 241
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Book Description
A broad, concise, and no-nonsense guide to contemporary electrophysiological techniques, covering intracellular and extracellular recording through recording of population activity, neuropharmacology, dye imaging, voltammetry, and optogenetics.
Author: Andrew I. Spielman
Publisher: CRC Press
ISBN: 9780849376450
Category : Science
Languages : en
Pages : 468
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Book Description
Experimental Cell Biology of Taste and Olfaction examines and adapts methods from a variety of established fields, such as neurophysiology, receptor biochemistry and cellular imaging to provide comprehensive coverage of current techniques and protocols in chemosensory cell biology. Written for both newcomers and established scientists, this volume offers numerous tips for problem solving and suggests ways to avoid the most common, and costly, mistakes made by researchers. This book covers general aspects such as tissue collection and preparation, as well as specific, up-to-date methods used in taste and olfactory morphology, immunology, biochemistry, biophysics, electrophysiology and molecular biology. The explosion of knowledge and the increased interest in these areas make this book an important reference work for all scientists, students, and teachers in this and related fields
Author:
Publisher: Academic Press
ISBN: 0080536468
Category : Science
Languages : en
Pages : 417
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Book Description
This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures. - A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies - Includes overviews of Optical probes and reagents - Fabrication and use of ion-selective microelectrodes - Use of NMR spectroscopy - Ionophores
Author: Ning Xi
Publisher: Artech House
ISBN: 1608071332
Category : Technology & Engineering
Languages : en
Pages : 311
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Book Description
Nanotechnology will allow us to build devices smaller than previously thought possible and will bring fundamental changes to disciplines within engineering, chemistry, medicine, biology, and physics. Understanding the principles of nano manipulation and assembly is tremendously important for those aiming to develop nanoscale systems. This forward-looking resource offers you cutting-edge coverage of the fundamentals and latest applications in this burgeoning field from an engineering perspective. The book shows you how nano-manipulation allows for the detection and manipulation of tiny entities such as single molecules, nanotubes, nanocubes, cells, viruses, proteins, and DNA molecules. You discover the most promising nanorobotic manipulation and assembly methods and find clear examples of key application areas, including nano sensors, electronics, and biomedical engineering. Supported with over 100 illustrations, this groundbreaking volumes offers comprehensive coverage of the subject, from the physics of nano manipulation... to robotic assembly of nano devices. Moreover, you get a glimpse of the future direction of this revolutionary technological area.
Author: Albert van den Berg
Publisher: Springer Science & Business Media
ISBN: 9401722641
Category : Science
Languages : en
Pages : 639
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Book Description
This volume contains the proceedings of the fourth international symposium on Micro Total Analysis Systems (muTAS 2000). Cutting-edge research of all invited and contributed papers presented by the world’s leading muTAS groups provides the state of the art of this electrifying, multidisciplinary field.
Author: Laszlo Zaborszky
Publisher: Springer Science & Business Media
ISBN: 1475720556
Category : Medical
Languages : en
Pages : 429
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Book Description
This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Author: Birendra N. Pramanik
Publisher: CRC Press
ISBN: 0824744195
Category : Science
Languages : en
Pages : 407
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Book Description
Discussing strategies to determine the structure and machanisms of numerous compound classics, this book covers new chemical and elctrophoretic techniques for rapid sample preconcentration and separation. It summarizes breakthroughs in the theory and instrumentation of electrospray mass spectrometry in pharmaceutical and biomedical applications, provides practical examples for the characterization of peptides, proteins, and glycoproteins, includes applications in proteomics, combinatorial chemistry, and drug characterization. Topics include chemical and electrophoretic techniques for rapid sample preconcentration and separation, screening processes for proteins from libraries of compounds, protein folding and dynamics, and more.
Author: Scott L. Hooper
Publisher: John Wiley & Sons
ISBN: 1118873629
Category : Medical
Languages : en
Pages : 690
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Book Description
A multi-disciplinary look at the current state of knowledge regarding motor control and movement—from molecular biology to robotics The last two decades have seen a dramatic increase in the number of sophisticated tools and methodologies for exploring motor control and movement. Multi-unit recordings, molecular neurogenetics, computer simulation, and new scientific approaches for studying how muscles and body anatomy transform motor neuron activity into movement have helped revolutionize the field. Neurobiology of Motor Control brings together contributions from an interdisciplinary group of experts to provide a review of the current state of knowledge about the initiation and execution of movement, as well as the latest methods and tools for investigating them. The book ranges from the findings of basic scientists studying model organisms such as mollusks and Drosophila, to biomedical researchers investigating vertebrate motor production to neuroengineers working to develop robotic and smart prostheses technologies. Following foundational chapters on current molecular biological techniques, neuronal ensemble recording, and computer simulation, it explores a broad range of related topics, including the evolution of motor systems, directed targeted movements, plasticity and learning, and robotics. Explores motor control and movement in a wide variety of organisms, from simple invertebrates to human beings Offers concise summaries of motor control systems across a variety of animals and movement types Explores an array of tools and methodologies, including electrophysiological techniques, neurogenic and molecular techniques, large ensemble recordings, and computational methods Considers unresolved questions and how current scientific advances may be used to solve them going forward Written specifically to encourage interdisciplinary understanding and collaboration, and offering the most wide-ranging, timely, and comprehensive look at the science of motor control and movement currently available, Neurobiology of Motor Control is a must-read for all who study movement production and the neurobiological basis of movement—from molecular biologists to roboticists.
