A Study of Transcriptional Regulation of the SUC2 Gene of S. Cerevisiae PDF Download
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Author: Heng Zhou
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages :
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Author: Heng Zhou
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages :
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Author: Laura E. Braly
Publisher:
ISBN:
Category : Ergosterol
Languages : en
Pages : 90
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Author: Christopher T. Harbison
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
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Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring considerable time and effort. Microarray technology now enables investigation of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a population of cells, as well as to locate the regions within the genome that are bound by transcriptional regulators. Such studies not only allow for the functional annotation of both genes and regulators, but can also provide clues about the identity of the regulatory regions within DNA, the structure of global regulatory networks and the regulation of DNA-binding proteins. These and other insights are presented here based on our genome-wide studies of transcriptional regulation in the yeast Saccharomyces cerevisiae.
Author: Eileen Mary Clarke
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 414
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Author: Ching-Ti Liu
Publisher:
ISBN: 9781109897388
Category :
Languages : en
Pages : 202
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Characterization of protein-protein interaction has become a key step in understanding the cellular mechanism because most biological processes cannot be carried out without protein-protein interaction. This dissertation focuses on a set of well-validated protein complex data from MIPS. It is commonly believed that functionally related genes tend to have similar expression patterns. However, we found notable contradictory evidence. Indeed, except for a few large protein complexes such as cytoplasmic or mitochondrial ribosomal proteins, the co-expression level of genes from the same complex is on the average not much higher than that from a pair of randomly selected genes. We then demonstrate the impact of these six protein complexes on other genes' regulation. The ways of incorporating the ChIP-on-Chip data is also studied by defining a pairwise co-regulation rate is defined for protein complexes. With this pairwise rate of co-regulation, we successfully link co-regulation and co-expression. To extend the concept of networks, we also study the relationship between protein complexes rather than proteins or genes alone. Specifically, we treat one complex or one group of genes as one node rather single gene. Liquid association is then applied to investigate the dynamic relationship between protein complexes.
Author: Ulrich Kück
Publisher: Springer Science & Business Media
ISBN: 3662103648
Category : Science
Languages : en
Pages : 372
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Mycology, the study of fungi, originated as a subdiscipline of botany and was a descriptive discipline, largely neglected as an experimental science until the early years of this century. A seminal paper by Blakeslee in 1904 provided evidence for self incompatibility, termed "heterothallism", and stimulated interest in studies related to the control of sexual reproduction in fungi by mating-type specificities. Soon to follow was the demonstration that sexually reproducing fungi exhibit Mendelian inheritance and that it was possible to conduct formal genetic analysis with fungi. The names Burgeff, Kniep and Lindegren are all associated with this early period of fungal genetics research. These studies and the discovery of penicillin by Fleming, who shared a Nobel Prize in 1945, provided further impetus for experimental research with fungi. Thus began a period of interest in mutation induction and analysis of mutants for bio chemical traits. Such fundamental research, conducted largely with Neurospora crassa, led to the one gene: one enzyme hypothesis and to a second Nobel Prize for fungal research awarded to Beadle and Tatum in 1958. Fundamental research in biochemical genetics was extended to other fungi, especially to Saccharomyces cere visiae, and by the mid-1960s fungal systems were much favored for studies in eukaryotic molecular biology and were soon able to compete with bacterial systems in the molecular arena.
Author: Tianwei Yu
Publisher:
ISBN:
Category :
Languages : en
Pages : 210
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Author: Ana Serra Barros
Publisher:
ISBN:
Category : Genomes
Languages : en
Pages : 442
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Author: Priyasri Shotton
Publisher:
ISBN:
Category :
Languages : en
Pages : 268
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DNA is the storehouse of genetic information for the normal functioning of all cellular processes. This DNA is "read" by RNA Polymerases (RNAP) to carry out the transcription process, which is one of the most vital regulatory mechanisms within the cell for gene expression. There are three important RNA Polymerases available in the cell. RNAPI synthesizes ribosomal RNAs, while RNAPII makes mRNA precursors, snRNAs and most microRNAs, additionally RNAPIII takes care of tRNAs, small RNAs and 5S rRNAs that occur in the nucleus and the cytosol. The work in the author's lab focuses on the function of RNAPII transcription. It is not straightforward for RNAPII to transcribe the DNA, it needs to struggle with two major roadblocks that are in its path. First, is the nucleosomal barrier that is scattered across the coding regions of active genes. The mechanism to overcome this major obstacle is a very complex process that involves an extensive range of proteins which work collectively to help in the alteration of chromatin structure that ultimately facilitates transcriptional regulation. In the thesis, the author has attempted to take a look at this intricate network and has studied the function of Rad26p, in yeast (associated with CSB-Cockayne syndrome B, in humans), a transcription coupled DNA repair factor, having an additional role in transcription. The results reveal that Rad26p plays a novel role in the regulation of elongation by modulating histone H2A-H2B dimer occupancy.
Author: Vince Harjono
Publisher:
ISBN:
Category :
Languages : en
Pages : 111
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Post-transcriptional regulation represents a powerful and versatile mechanism to fine-tune gene expression to meet cellular and environmental demands. One important aspect of post-transcriptional regulation involves regulation of protein translation, the process of building proteins from a messenger RNA. In this dissertation, I use biochemical and molecular biology techniques to study how translation is mechanistically regulated by both mRNA and protein factors. In chapter 2, I discuss the development of a quantitative method in eukaryotes to measure ribosomal stalls of cis-mRNA factors on protein elongation. We find that different distributions of nonoptimal codons trigger different surveillance and rescue pathways despite similar levels of elongation delay. In chapter 3, I explore the relationship between translatability and mRNA localization during glucose starvation and investigate potential factors that influence this relationship. We find that a complex made from Rvb1 and Rvb2 is involved in promoter-directed cytoplasmic fate in a subset of stress response genes in glucose starvation. Furthermore, we use carefully designed reporters to interrogate how translatability determines cytoplasmic localization and find that active translation is linked to exclusion from stress-induced cytoplasmic granules. Finally in chapter 4, I discuss improvements on the method we have developed, possible future directions for the work described in this dissertation, and my concluding remarks.
