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Author: John R. Crowther
Publisher: Humana
ISBN: 9781617378843
Category : Medical
Languages : en
Pages : 0
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Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: John R. Crowther
Publisher: Humana
ISBN: 9781617378843
Category : Medical
Languages : en
Pages : 0
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Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: D. M. Kemeny
Publisher: Pergamon
ISBN:
Category : Medical
Languages : en
Pages : 132
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Book Description
This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
Author: David Wild
Publisher: Newnes
ISBN: 0080970389
Category : Science
Languages : en
Pages : 1037
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Book Description
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Author: Robert Hnasko
Publisher: Humana
ISBN: 9781493927418
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Author: Lisa Barron
Publisher: IAP
ISBN: 1641138173
Category : Education
Languages : en
Pages : 471
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Book Description
edTPA is the most widely-used performance assessment for pre-service teachers in the United States, and a requirement in many states for teaching licensure. Through edTPA, teacher candidates demonstrate their effectiveness in different aspects of teaching, including planning, instruction, assessment, analysis of teaching, and use of academic language. This book is a practical guide for anyone involved in edTPA implementation. The chapters are written by experienced teacher educators who are leading successful edTPA programs in their own universities, who are in the field, and involved in the work. They represent diverse teacher preparation programs, each with their own strengths and challenges. This book addresses the challenges of edTPA, while providing practical strategies for educative and thoughtful implementation. Organized into four sections, each section explores a different aspect of edTPA implementation, and provides guidance for leading faculty and teacher candidates through edTPA.
Author: John R. Crowther
Publisher: Springer Science & Business Media
ISBN: 1592595294
Category : Medical
Languages : en
Pages : 231
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Book Description
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Author: Elisa Romeo
Publisher: Hay House, Inc
ISBN: 1401947654
Category : Self-Help
Languages : en
Pages : 265
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Book Description
In this groundbreaking book, therapist and intuitive Elisa Romeo, takes Soul beyond an intellectual concept and into a direct and personal relationship. Pulling from depth psychology, years of practical experience, and touching stories from clients, Elisa brings substance and gravitas—and some levity—to the topic of how to live a Soulful life. This comprehensive, straightforward program contains effective meditations, visualizations, and inquiries to support you on the often messy, winding, and yet amusing path of discovering your Soul in modern times. Meet Your Soul not only tells you how to access your Soul connection but also preps you for some of the common obstacles you’re likely to encounter on your journey. With her kind and informed direction, you’ll learn to: • Understand the crucial distinction between ego, Spirit, and Soul • Create and cultivate a strong spiritual practice • Distinguish your Soul Truth from the beliefs of family, friends, and society • Clearly access the voice of your Soul • Gain awareness of the key Soul contracts in your life and discover your Divine purpose This book helps you meet the oldest, wisest, and most loving part of yourself—the part that gives meaning and purpose to your life. Elisa shows that each of us has the capacity to hear this personal, unique guidance directly. You needn’t look outside yourself to a guru, program, or system; rather you can simply look within.
Author: Elisa J Sobo
Publisher: Routledge
ISBN: 1315430916
Category : Social Science
Languages : en
Pages : 329
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Book Description
Culture and Meaning in Health Services Research is a practical guide to applying interpretive qualitative methods to pressing healthcare delivery problems. A leading medical anthropologist who has spent many years working in applied healthcare settings, Sobo combines sophisticated theoretical insights and methodological rigor with authentic, real-world examples and applications. In addition to clearly explaining the nuanced practice of ethnography and guiding the reader through specific methods that can be used in focus groups or interviewing to yield useful findings, Sobo considers the social relationships and power dynamics that influence field entry, data ownership, research deliverables, and authorship decisions. Crafted to communicate the importance of culture and meaning across the many disciplines engaged in health services research, this book is ideal for courses in such fields as public health and health administration, nursing, anthropology, health psychology, and sociology.
Author:
Publisher: Academic Press
ISBN: 012375173X
Category : Science
Languages : en
Pages : 961
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Book Description
This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. - Provides up-to-date methods necessary to study genes in yeast - Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations - Serves as an essential reference for any beginning or experienced researcher in the field
Author: Richard B. Ford
Publisher: Elsevier Health Sciences
ISBN: 1437707998
Category : Medical
Languages : en
Pages : 787
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Book Description
Provide expert care for cats and dogs! Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment, 9th Edition covers not only the management of emergency conditions, but also strategies for dealing with hundreds of routine diagnostic and treatment challenges in small animals. Its user-friendly format provides instant access to vital information -- making it an ideal resource in emergency situations -- and it is conveniently organized by both body systems and presenting signs to help you easily reach a diagnosis and determine a treatment plan for all clinical situations. Written by veterinary experts Richard Ford and Elisa Mazzaferro, Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment provides current guidelines for small animal emergency care and the diagnostic procedures most commonly performed in a busy, team-oriented practice. - Step-by-step instructions and illustrations are provided for all major emergency and non-emergency clinical procedures. - A logical, easy-to-use format lists all emergency conditions in alphabetical order, and includes quick reference boxes calling out key information such as clinical tips and cautions. - Clear, concise guidelines help you evaluate clinical signs and laboratory test data. - Clinical algorithms make it easier to identify and treat abnormalities. - Guidelines for assessment and treatment include practical advice and solutions, how to examine the small animal patient using a body systems and problem list approach, and a review of basic diagnostic procedures used in daily practice. - Coverage of toxicological emergencies describes how to manage exposures and poisonings. - A quick reference guide to the management of the emergency patient is conveniently located on the inside cover. - A comprehensive drug formulary makes lookup easy, and includes proprietary names, actions/use of each drug, formulations, recommended dosages, and special precautions, with emergency medications highlighted for fast reference - This all-in-one reference includes practical coverage of emergency procedures, physical assessment in sickness and health, routine and advanced testing procedures, diagnostic tests sampling, preparation, procedures, and interpretation. - Quick Reference boxes include potential causes of each clinical abnormality and associated signs, step-by-step diagnostic plans, and clinical algorithms. - The latest vaccination guidelines include protocols for dogs and cats at low, medium, and high risk of exposure to infectious diseases. - Updated coverage keeps you current with the latest on pain assessment, prevention, and treatment.
