A Phase Contrast Imaging Technique for Near-field Scanning Optical Microscopy PDF Download
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Author: Andrew David Woodfin
Publisher:
ISBN:
Category :
Languages : en
Pages : 140
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Book Description
ABSTRACT: A technique allowing for phase contrast imaging with a near-field scanning optical microscope is discussed. Background theory relating to near-field scanning optical microscopy (NSOM) is presented, in addition to relevant aspects of interferometry. A brief discussion of currently available methods for phase contrast imaging in both near-field and far-field microscopy is presented. Theory regarding the proposed technique, which exploits the interference of electrooptically modulated light and a conventional NSOM signal, is discussed in detail. Phase data using the proposed technique in a simplified configuration is presented to validate its effectiveness. The experimental arrangement used for data collection is specified. Finally, phase data obtained using the proposed technique in conjunction with a near-field microscope is examined and assessed in comparison with the results expected from prior knowledge of the sample under study.
Author: Andrew David Woodfin
Publisher:
ISBN:
Category :
Languages : en
Pages : 140
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Book Description
ABSTRACT: A technique allowing for phase contrast imaging with a near-field scanning optical microscope is discussed. Background theory relating to near-field scanning optical microscopy (NSOM) is presented, in addition to relevant aspects of interferometry. A brief discussion of currently available methods for phase contrast imaging in both near-field and far-field microscopy is presented. Theory regarding the proposed technique, which exploits the interference of electrooptically modulated light and a conventional NSOM signal, is discussed in detail. Phase data using the proposed technique in a simplified configuration is presented to validate its effectiveness. The experimental arrangement used for data collection is specified. Finally, phase data obtained using the proposed technique in conjunction with a near-field microscope is examined and assessed in comparison with the results expected from prior knowledge of the sample under study.
Author: D.W. Pohl
Publisher: Springer Science & Business Media
ISBN: 9401119783
Category : Science
Languages : en
Pages : 426
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Book Description
Scanning near-field optical microscopy (SNOM, also known as NSOM) is a new local probe technique with a resolving power of 10--50 nm. Not being limited by diffraction, near-field optics (NFO) opens new perspectives for optical characterization and the understanding of optical phenomena, in particular in biology, microelectronics and materials science. SNOM, after first demonstrations in '83/'84, has undergone a rapid development in the past two to four years. The increased interest has been largely stimulated by the wealth of optical properties that can be investigated and the growing importance of characterization on the nanometer scale in general. Examples include the use of fluorescence, birefrigence and plasmon effects for applications in particular in biology, microelectronics and materials science, to name just a few. This volume emerged from the first international meeting devoted exclusively to NFO, and comprises a complete survey of the 1992 activities in the field, in particular the variety of instrumental techniques that are currently being explored, the demonstration of the imaging capabilities as well as theoretical interpretations - a highly nontrivial task. The comprehensive collection of papers devoted to these and related subjects make the book a valuable tool for anybody interested in near-field optics.
Author: Gordon S. Kino
Publisher: Academic Press
ISBN: 008052978X
Category : Science
Languages : en
Pages : 353
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Book Description
This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications Discusses the theory and design of near-field optical microscopes Explains phase imaging in the scanning optical and interference microscopes
Author: Simon Zabler
Publisher: MDPI
ISBN: 3038972843
Category : Image processing
Languages : en
Pages : 147
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Book Description
This book is a printed edition of the Special Issue "Phase-Contrast and Dark-Field Imaging" that was published in J. Imaging
Author: Ricardo Toledo-Crow
Publisher:
ISBN:
Category : Imaging systems
Languages : en
Pages : 366
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Book Description
"This dissertation describes the force regulated near-field scanning optical microscope (NSOM) and two important adaptations: one for imaging polarization contrast with a linear response, and the other for interference imaging. An introduction to near- field optics is first presented, followed by a description of the background to this work that includes the relevant references to the literature and previous results. A simple theoretical description of the NSOM in terms of scalar fields is then presented, followed by an exposition of an early but relevant rigorous vectorial interpretation of the experiment. The basic force regulated NSOM is presented: its parts, operation, and construction is described and discussed, and its imaging capabilities are shown and discussed with results from different samples. In particular, the ability of the system to simultaneously image topographical and optical characteristics of the samples is described, and the importance of separating the optical and topographical information for the correct operation of the microscope is stressed. An adaptation to the basic NSOM that permits imaging sample-dependent polarization variations with sub-wavelength resolution and with a linear sensitivity is then discussed and analyzed. Results are shown for several samples that include metal on quartz, magneto-optic media, and polymers. Lastly, an interferometric arrangement of the NSOM is presented that allows imaging of phase variations, as well as polarization variations, with a significant signal enhancement achieved with the use of a pseudo-heterodyning technique."--Abstract.
Author: Vladimir N Uversky
Publisher: Academic Press
ISBN: 0123978211
Category : Science
Languages : en
Pages : 556
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Book Description
Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers - Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme aggregation, misfolded intermediate of a PDZ domain, and micelle formation by human islet amyloid polypeptide Protein misfolding and aggregation is a fast-growing frontier in molecular medicine and protein chemistry. Related disorders include cataracts, arthritis, cystic fibrosis, late-onset diabetes mellitus, and numerous neurodegenerative diseases like Alzheimer's and Parkinson's. Nanoimaging technology has proved crucial in understanding protein-misfolding pathologies and in potential drug design aimed at the inhibition or reversal of protein aggregation. Using these technologies, researchers can monitor the aggregation process, visualize protein aggregates and analyze their properties. Provides practical examples of nanoimaging research from leading molecular biology, cell biology, protein chemistry, biotechnology, genetics, and pharmaceutical labs Includes over 200 color images to illustrate the power of various nanoimaging technologies Focuses on nanoimaging techniques applied to protein misfolding and aggregation in molecular medicine
Author: Gerd Kaupp
Publisher: Springer Science & Business Media
ISBN: 3540284729
Category : Technology & Engineering
Languages : en
Pages : 302
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Book Description
Making a clear distinction is made between nano- and micro-mechanical testing for physical reasons, this monograph describes the basics and applications of the supermicroscopies AFM and SNOM, and of the nanomechanical testing on rough and technical natural surfaces in the submicron range down to a lateral resolution of a few nm. New or improved instrumentation, new physical laws and unforeseen new applications in all branches of natural sciences (around physics, chemistry, mineralogy, materials science, biology and medicine) and nanotechnology are covered as well as the sources for pitfalls and errors. It outlines the handling of natural and technical samples in relation to those of flat standard samples and emphasizes new special features. Pitfalls and sources of errors are clearly demonstrated as well as their efficient remedy when going from molecularly flat to rough surfaces. The academic or industrial scientist learns how to apply the principles for tackling their scientific or manufacturing tasks that include roughness far away from standard samples.
Author: National Research Council
Publisher: National Academies Press
ISBN: 030916463X
Category : Science
Languages : en
Pages : 222
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Book Description
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Author: Michael Sean Riley
Publisher:
ISBN:
Category :
Languages : en
Pages : 250
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Book Description
The near-field scanning optical microscope (NSOM) is optical microscope that exploits highly localized interactions between a light emitting sub-wavelength aperture and a microscopic object to achieve optical spatial resolution that can approach 30nm. Conventional NSOM methods yield excellent topography and intensity images of opaque materials, where there is significant amplitude alternation to the incident field to provide contrast. Although the light-sample interactions, associated with NSOM, are highly localized, transparent material is either partially resolved or not resolved at all. A conventional NSOM was integrated into an environmentally stabilized Mach-Zehnder interferometer, part optical fiber, and part air, to determine phase changes induced by refractive-index variations in materials. The completed NSOM system allows for simultaneous phase, topography, and intensity imaging of microscopic objects. A 380nm thick transparent pattern photo-resist sample, which consisted of 2um circles on 4um centers, was imaged. The results clearly show an enhanced image quality in the phase image when compared to the intensity image. The resolution was found to be not only function of the aperture size, but a function of the uniformity of the output field emitted by the sub-wavelength aperture and the modulation amplitude of the resonating probe tip-fork system that is employed to maintain the sub-wavelength aperture probe tip in the near-field.
Author: S. Bradbury and P.J. Evennett
Publisher: Garland Science
ISBN: 1000144607
Category : Medical
Languages : en
Pages : 195
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Book Description
Contrast in an image is essential to distinguish features from one another and from the background. This practical handbook describes the ways in which light interacts with the specimen in the microscope.
