A Nonradioactive Nucleic Acid Labeling Method and a Detection Method Using it PDF Download
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Author: T. Takahashi
Publisher:
ISBN:
Category :
Languages : en
Pages : 7
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Book Description
Author: T. Takahashi
Publisher:
ISBN:
Category :
Languages : en
Pages : 7
Get Book Here
Book Description
Author: Christoph Kessler
Publisher: Springer
ISBN: 3662001446
Category : Science
Languages : en
Pages : 449
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Book Description
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author: Elena Hilario
Publisher: Springer Science & Business Media
ISBN: 1597452297
Category : Science
Languages : en
Pages : 318
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Book Description
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author: Larry J. Kricka
Publisher: Academic Press
ISBN: 0323137717
Category : Science
Languages : en
Pages : 373
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Book Description
Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided. This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection. Gives extensive practical information Covers major types of nonisotopic labels and procedures Presents background information for each method Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by Fluorescence Chemiluminescence Bioluminescence Colorimetry
Author: A. J. Garman
Publisher: Elsevier
ISBN: 0080551092
Category : Science
Languages : en
Pages : 155
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Book Description
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. Aimed at researchers without specific expertise in chemistry, the book includes: A review of the main signal systems and labels available, indicating their strengths and weaknesses Discussion of the most useful strategies for labelling the various biomolecules 32 protocols covering common labelling needs Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications Sources of information including references, data and suppliers
Author: Christoph Kessler
Publisher: Springer Science & Business Media
ISBN: 3642572065
Category : Science
Languages : en
Pages : 759
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Book Description
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.
Author: Gary C. Howard
Publisher: McGraw-Hill/Appleton & Lange
ISBN: 9780444016614
Category : Science
Languages : en
Pages : 342
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Book Description
Protein methods. Biotin labeling methods for proteins and carbohydrates. Direct conjugation of enzymes to proteins. Labeling proteins with fluorochromes. Comparing fluorescent organic dyes for biomolecular labeling. Chemiluminescent detection using alkaline phosphatase-the chemistry. Detection of proteins on filters by enzymatic methods. Enzyme-linked immunosorbent assays. Automation of protein and immunochemistry methods. DNA methods. Biotin labeling methods for DNA. Direct attachment of enzymes to DNA probes. Detection of nucleic acids on filters by enzymatic methods. Polymerase chain reaction and its impact on DNA detection limits. Automated procedures for DNA methods. Tissue and cell methods. Enzyme-based methods in tissue sections. In situ hybridization. Chromosome mapping by fluorescent in situ hybridization. Colloidal gold localization of in situ hybrids. Immunofluorescence methods for microscopic analysis. Flow cytometry methods.
Author: Christoph Kessler
Publisher: Springer Science & Business Media
ISBN: 9783540646013
Category : Science
Languages : en
Pages : 1170
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Book Description
Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author: Robert H. Symons
Publisher: CRC Press
ISBN: 9780849349423
Category : Medical
Languages : en
Pages : 246
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Book Description
With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes with radioisotopes and with non-radioactive ligands and haptens are discussed. Additionally, the associated methods for their detection, the use of these probes in the diagnosis of human and microbial pathogens, of plant viruses and viroids, and of human genetic disorders, as well as in the detection of nucleic acids in tissues and cells by in situ hybridization are presented.
Author: P. Lebacq
Publisher:
ISBN:
Category :
Languages : en
Pages : 18
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Book Description
